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EC number: 260-925-2 | CAS number: 57741-47-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, repeated dose oral toxicity was predicted for the test compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl} -4-hydroxynaphthalene -2-sulfonate (57741-47-6). The study assumed the use of male and female Crj: CD(SD) in chronic study of 90days. No significant alterations were noted at the dose level of 667.080mg/Kg bw/day. The predicted No Observed Adverse Effect Level (NOAEL) for compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl) phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate is considered to be 450.2mg/Kg bw/day. Based on this value it can be concluded that the substance is considered to not toxic as per the criteria mentioned in CLP regulation.
Key value for chemical safety assessment
Repeated dose toxicity: via oral route - systemic effects
Link to relevant study records
- Endpoint:
- repeated dose toxicity: oral
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- Prediction is done using OECD QSAR Toolbox version 3.3 and the supporting QMRF report has been attached.
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- Prediction is done using OECD QSAR Toolbox version 3.3, 2018.
- GLP compliance:
- not specified
- Limit test:
- no
- Specific details on test material used for the study:
- - Name of test material : Sodium 6-amino-5-[[4-chloro-2-(trifluoromethyl)phenyl]azo]-4-hydroxynaphthalene-2-sulphonate
- Molecular formula : C17H10ClF3N3NaO4S
- Molecular weight : 467.786 g/mol
- Smiles notation : c12c(cc(S(=O)(=O)[O-])cc2O)ccc(c1\N=N/c1c(cc(Cl)cc1)C(F)(F)F)N.[Na+]
- InChl : 1S/C17H11ClF3N3O4S.Na/c18-9-2-4-13(11(6-9)17(19,20)21)23-24-16-12(22)3-1-8-5-10(29(26,27)28)7-14(25)15(8)16;/h1-7,25H,22H2,(H,26,27,28);/q;+1/p-1/b24-23-;
- Substance type : Organic
- Physical state : Solid - Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- not specified
- Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 90 days
- Frequency of treatment:
- not specified
- Remarks:
- not specified
- No. of animals per sex per dose:
- not specified
- Control animals:
- not specified
- Details on study design:
- not specified
- Positive control:
- not specified
- Observations and examinations performed and frequency:
- not specified
- Sacrifice and pathology:
- not specified
- Other examinations:
- not specified
- Statistics:
- not specified
- Clinical signs:
- not specified
- Mortality:
- not specified
- Body weight and weight changes:
- not specified
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- not specified
- Clinical biochemistry findings:
- not specified
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not specified
- Immunological findings:
- not specified
- Organ weight findings including organ / body weight ratios:
- not specified
- Gross pathological findings:
- not specified
- Neuropathological findings:
- not specified
- Histopathological findings: non-neoplastic:
- not specified
- Histopathological findings: neoplastic:
- not specified
- Other effects:
- not specified
- Dose descriptor:
- NOAEL
- Effect level:
- 450.238 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: NO significant effect were observed at this dose .
- Remarks on result:
- other: No toxic effect were observed a this dose .
- Critical effects observed:
- not specified
- Treatment related:
- not specified
- Dose response relationship:
- not specified
- Relevant for humans:
- not specified
- Conclusions:
- The predicted No Observed Adverse Effect Level (NOAEL) for Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6) is considered to be 450.2mg/Kg bw/day.
- Executive summary:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, repeated dose oral toxicity was predicted for the test compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6). The study assumed the use of male and female Crj: CD(SD) in chronic study of 90days. No significant alterations were noted at the dose level of 667.080mg/Kg bw/day. The predicted No Observed Adverse Effect Level (NOAEL) for compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl) phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate is considered to be 450.2mg/Kg bw/day. Based on this value it can be concluded that the substance is considered to not toxic as per the criteria mentioned in CLP regulation.
Reference
The
prediction was based on dataset comprised from the following
descriptors: NOAEL
Estimation method: Takes average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain
(((((((("a"
or "b" or "c" or "d" or "e" )
and ("f"
and (
not "g")
)
)
and ("h"
and (
not "i")
)
)
and ("j"
and (
not "k")
)
)
and ("l"
and (
not "m")
)
)
and ("n"
and (
not "o")
)
)
and ("p"
and (
not "q")
)
)
and ("r"
and "s" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Anilines (Acute toxicity) by
US-EPA New Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Non-covalent interaction AND
Non-covalent interaction >> DNA intercalation AND Non-covalent
interaction >> DNA intercalation >> Fused-Ring Primary Aromatic Amines
AND Radical AND Radical >> Radical mechanism via ROS formation
(indirect) AND Radical >> Radical mechanism via ROS formation (indirect)
>> Fused-Ring Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic
attack after metabolic nitrenium ion formation AND SN1 >> Nucleophilic
attack after metabolic nitrenium ion formation >> Fused-Ring Primary
Aromatic Amines by DNA binding by OASIS v.1.3
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion
formation AND SN1 >> Nitrenium Ion formation >> Aromatic azo AND SN1 >>
Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Strong binder, NH2 group AND
Strong binder, OH group by Estrogen Receptor Binding
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Acid moiety AND Anilines
(Unhindered) AND Phenol Amines AND Phenols AND Salt by Aquatic toxicity
classification by ECOSAR
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Not categorized by Repeated dose
(HESS)
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as p-Aminophenols (Renal toxicity)
Rank B by Repeated dose (HESS)
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Not categorized by Repeated dose
(HESS)
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Nitrobenzenes (Hepatotoxicity)
Rank C by Repeated dose (HESS)
Domain
logical expression index: "j"
Referential
boundary: The
target chemical should be classified as Not categorized by Repeated dose
(HESS)
Domain
logical expression index: "k"
Referential
boundary: The
target chemical should be classified as Anilines (Hepatotoxicity) Rank C
by Repeated dose (HESS)
Domain
logical expression index: "l"
Referential
boundary: The
target chemical should be classified as Alkyl fluoride AND Alkyl halide
AND Amine AND Anion AND Aromatic compound AND Aryl chloride AND Aryl
halide AND Azo compound AND Cation AND Halogen derivative AND Hydroxy
compound AND Phenol AND Primary amine AND Primary aromatic amine AND
Sulfonic acid derivative by Organic functional groups, Norbert Haider
(checkmol)
Domain
logical expression index: "m"
Referential
boundary: The
target chemical should be classified as Alcohol by Organic functional
groups, Norbert Haider (checkmol)
Domain
logical expression index: "n"
Referential
boundary: The
target chemical should be classified as Alkyl fluoride AND Alkyl halide
AND Amine AND Anion AND Aromatic compound AND Aryl chloride AND Aryl
halide AND Azo compound AND Cation AND Halogen derivative AND Hydroxy
compound AND Phenol AND Primary amine AND Primary aromatic amine AND
Sulfonic acid derivative by Organic functional groups, Norbert Haider
(checkmol)
Domain
logical expression index: "o"
Referential
boundary: The
target chemical should be classified as Carbonic acid derivative by
Organic functional groups, Norbert Haider (checkmol)
Domain
logical expression index: "p"
Referential
boundary: The
target chemical should be classified as Alkyl fluoride AND Alkyl halide
AND Amine AND Anion AND Aromatic compound AND Aryl chloride AND Aryl
halide AND Azo compound AND Cation AND Halogen derivative AND Hydroxy
compound AND Phenol AND Primary amine AND Primary aromatic amine AND
Sulfonic acid derivative by Organic functional groups, Norbert Haider
(checkmol)
Domain
logical expression index: "q"
Referential
boundary: The
target chemical should be classified as No functional group found by
Organic functional groups, Norbert Haider (checkmol)
Domain
logical expression index: "r"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= -2.09
Domain
logical expression index: "s"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 3.86
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- NOAEL
- 450.2 mg/kg bw/day
- Study duration:
- chronic
- Species:
- rat
- Quality of whole database:
- Data is from K2 prediction OECD QSAR toolbox 3.3.
Repeated dose toxicity: inhalation - systemic effects
Endpoint conclusion
- Endpoint conclusion:
- no study available
Repeated dose toxicity: inhalation - local effects
Endpoint conclusion
- Endpoint conclusion:
- no study available
Repeated dose toxicity: dermal - systemic effects
Endpoint conclusion
- Endpoint conclusion:
- no study available
Repeated dose toxicity: dermal - local effects
Endpoint conclusion
- Endpoint conclusion:
- no study available
Mode of Action Analysis / Human Relevance Framework
Repeated dose toxicity: via oral route
Various experimental studies were reviewed to determine the toxic nature of target substance Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4- hydroxynaphthalene-2-sulfonate (57741-47-6)upon repeated exposure by oral route. The studies are as mentioned below:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, repeated dose oral toxicity was predicted for the test compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6). The study assumed the use of male and female Crj: CD(SD) in chronic study of 90days. No significant alterations were noted at the dose level of 667.080mg/Kg bw/day. The predicted No Observed Adverse Effect Level (NOAEL) for compound Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl) phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate is considered to be 450.2mg/Kg bw/day. Based on this value it can be concluded that the substance is considered to not toxic as per the criteria mentioned in CLP regulation.
Another chronic toxicity study for Read across was performed by Ministry of Health, Labour and Welfare", Scientific Committee on Cosmetology (Environment, Consumer Protection and Nuclear Safety, 1988) to determine the oral toxic nature of D&C Red 33; IUPAC name disodium 5-amino-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (3567-66-6). The read across substances share high similarity in structure and log kow .Therefore, it is acceptable to derive information on mutation from the analogue substance. In a chronic repeated dose toxicity study, dogs were treated with Disodium salt of 8-amino-2-phenylazo-1-naphtoL-3, 6-disulphonic acid in the concentration of 750 mg/kg bw/day orally in diet. Histopathological changes in the Liver, kidneys and thyroid were observed in 750 mg/kg/day treated dogs No other effect in clinical sign, mortality ,morbidity were observed at this dose. Therefore, NOAEL was considered to be 750 mg/kg bw/day when dogs were treated with Disodium salt of 8-amino-2-phenyLazo-1-naphtoL-3, 6-disuLphonic acid orally for 90 days.
Repeated inhalation study:
According to Annex IX of the REACH regulation, testing by the inhalation route is appropriate only if exposure of humans via inhalation is likely. Taking into account the low vapour pressure of the substance Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6) ,which is reported as 1.5598E-19Pa at 25 C. Also considering the particle size distribution of the substance the majority of the particles was found to be in the size of 150 micron to 25 micron which is much larger size range compared to the inhalable particulate matter. Thus, exposure to inhalable dust, mist and vapour of the chemical Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate is highly unlikely. Therefore this study is considered for waiver.
Repeated dermal study
The acute toxicity value for Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6) (as provided in section 7.2.3) is 3204mg/kg body weight. Also, given the use of the chemical; repeated exposure by the dermal route is unlikely since the use of gloves is common practice in industries. Thus, it is expected that Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate shall not exhibit 28 day repeated dose toxicity by the dermal route. In addition, there is no data available that suggests that Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate shall exhibit repeated dose toxicity by the dermal route. Hence this end point was considered for waiver.
Based on the data available for the target chemical and its prediction, Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6) does not exhibit toxic nature upon repeated exposure by oral, inhalation and dermal route of exposure and hence is not likely to classify as per the criteria mentioned in CLP regulation.
Additional information
Based on the data available for the target chemical and its prediction, Sodium 6-amino-5-{[4-chloro-2-(trifluoromethyl)phenyl]diazenyl}-4-hydroxynaphthalene-2-sulfonate (57741-47-6) does not exhibit toxic nature upon repeated exposure by oral, inhalation and dermal route of exposure and hence is not likely to classify as per the criteria mentioned in CLP regulation.
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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