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EC number: 204-429-6 | CAS number: 120-83-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Method similar to OECD 471 except certain E. coli strains missing.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- E. col wpA2 strains not included
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,4-dichlorophenol
- EC Number:
- 204-429-6
- EC Name:
- 2,4-dichlorophenol
- Cas Number:
- 120-83-2
- Molecular formula:
- C6H4Cl2O
- IUPAC Name:
- 2,4-dichlorophenol
- Details on test material:
- Received as coded aliquots from Radian Corporation, Texas, USA
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction from Syrian hamster or Sprague Dawley rat Aroclor 1254 induced liver
- Test concentrations with justification for top dose:
- 0, 3.3, 10, 33, 100, 333 µg/plate
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Remarks:
- 2-aminoanthracene, 4-nitro-o-phenylediamine, sodium azide
- Details on test system and experimental conditions:
- 2,4-DCP in addition to the solvent and positive controls were tested in the presence or absence of S-9 mix. 3 replicates were used. The experiment was repeated no less than 1 week after completion of the initial test. Liver S9 fraction were prepared from male Sprague Dawley rats and Syrian hamster that were induced with Aroclor 1254 5 days before sacrifice.
- Evaluation criteria:
- A positive repsonse was defined as a reproducible, dose related increase in histidine-independent (revertant) colonies in any one strain/activation combination. An equivocal response was defined as an increase in revertants which was not dose related, not reproducible, or of insufficient magnitude to support a determination of mutagenicity. A reponse was considered negative when no increase in revertant colonies was observed after chemical treatment.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Slight toxicity seen at highest dose tested (333 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at highest dose tested 333 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA 1537, TA98 and TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at highest dose tested (333 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The equivocal or ambiguous result see with metabolic activation in TA 1535 was seen in the presence of Hamster derived S9 only and NOT with rat derived S9.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
ambiguous with metabolic activation Strain 1535 gave equivocal results in the presence of hamster derived S9 only (and not rat derived S9)).
2,4 dichlorophenol gave an ambiguous result in strain TA 1535 in the presence of Hamster derived S9 only. Results obtained in all other scenarios examined were negative. - Executive summary:
In a reverse gene mutation assay in bacteria, strains TA 98, TA 100, TA 1535, TA1537 of S. typhimurium were exposed to 2,4-dichlorophenol, at concentrations of 0, 3.3, 10, 33, 100, 333 µg/plate (co-incubation) in the presence and absence of mammalian metabolic activation derived from both Aroclor 1254 induced Sprague Dawley rat and Syrian hamster liver.
2,4-dichlorophenol was tested up to cytotoxic concentrations (300 µg/plate). The positive controls induced the appropriate responses in the corresponding strains. There was some evidence of induced mutant colonies over background (2 fold) for strain TA 1535 at the top dose (i.e. in the presence of cytotoxity) in the presence of Syrian hamster S9 but not rat S9.
This study is classified as acceptable and satisfies the requirement of Test Guideline OECD 471 for in vitro mutagenicity (bacterial reverse gene mutation) data with the exception that certain E. coli strains were missing.
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