5-methyl-s-triazolo[1,5-a]pyrimidin-7-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 February 1985 to 01 May1985

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-methyl-s-triazolo[1,5-a]pyrimidin-7-ol

EC Number:

219-706-7

EC Name:

5-methyl-s-triazolo[1,5-a]pyrimidin-7-ol

Cas Number:

2503-56-2

Molecular formula:

C6H6N4O

IUPAC Name:

5-methyl-s-triazolo[1,5-a]pyrimidin-7-ol

Test material form:

solid: particulate/powder

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Cpb/TNO Zeist, The Netherlands
- Weight at study initiation: 2478-2742 g
- Housing: individually in metal cages with perforated floors
- Diet: standard diet ( LK01 Hope Farms Woerden) 100-120 g/d
- Water: tap water ad libitum
- Acclimation period: 3 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20±1 °C
- Humidity (%): 45-55%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light):12/12

Test system

Vehicle:

unchanged (no vehicle)

Remarks:

grounded before instillation

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 58 mg (slightly below 0.1 mL) ground to a fine powder

VEHICLE: none

Duration of treatment / exposure:

NA

Observation period (in vivo):

at 1, 24, 48 and 72 hours after instillation (for one rabbit a 9 day observation was included)

Number of animals or in vitro replicates:

3 females

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): none

SCORING SYSTEM: Draize

TOOL USED TO ASSESS SCORE: at 24 hfluorescein staining was used

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Remarks on result:

no indication of irritation

Remarks:

fluorescein examination did not show any damage

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Results 3

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

mean

Time point:

24/48/72 h

Score:

1.4

Max. score:

2

Reversibility:

fully reversible within: 9 days

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0.1

Max. score:

2

Reversibility:

fully reversible

Any other information on results incl. tables

Animal no	1				2				3
	1	24	48	72	1	24	48	72	1	24	48	72
cornea	0	0	0	0	0	0	0	0	0	0	0	0
iris	0	0	0	0	0	0	0	0	0	0	0	0
conjunctivae
redness	1	0	0	0	1	1	1	1	1	1	1	0
chemosis	0	0	0	0	2	1	0	1	2	0	0	0

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The substance is considered non-irritant to the eyes

Executive summary:

Three female rabbits received 58 mg (slightly below 0.1 mL) of the substance in the eye. The eyes were observed for signs of irritation after 1, 24, 48 and 72 hours. No effects were found on the cornea and the iris. The effects on the conjunctivae were limited and fully reversible at an additional observation after 9 days. The substance is considered not irritating to the eyes.