Methylcyclopentadiene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well conducted, GLP compliant, study undertaken according to internationally recognised test methods

Data source

Reference

Reference Type:

other: USEPA High Production Volume Information System (HPVIS)

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methylcyclopentadiene

EC Number:

247-724-5

EC Name:

Methylcyclopentadiene

Cas Number:

26472-00-4

Molecular formula:

not applicable, UVCB substance

IUPAC Name:

3a,4,7,7a-tetrahydro-1H-4,7-methanoindene

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Water accomodated fraction (WAF)

Test solutions

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc):

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Age of inoculum (at test initiation): 5 days, in log-phase growth

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

Mean test temperature: 23.4±0.2°C

pH:

7.6 in all of the test solutions at test initiation and within the range 8.8 to 10.1 on termination of the test after 96 hours.

Nominal and measured concentrations:

Nominal /Measured (mg/L): 0 / 0; 0.019 / 0.029; 0.068 / 0.048; 0.17 / 0.096; 0.51 / 0.28; 1.1 / 0.76

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, 125 mL, no headspace, 140 mL
- Aeration: No
- Initial cells density: 1.0E4 cells/mL
- No. of vessels per concentration (replicates): 12
- No. of vessels per control (replicates): 12

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Photoperiod: Continous light
- Light intensity and quality: 8400 - 8636 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Daily in triplate

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.82 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 0.63 - 1.3 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.42 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 0.23 - 0.91 mg/L

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.096 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.096 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 5

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.83 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 0.71 - 1.1 mg/L

Effect concentrations 6

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.42 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 0.25 - 0.86 mg/L

Effect concentrations 7

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.066 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 8

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.096 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance is very toxic to aquatic organisms

Executive summary:

The test substance is very toxic to aquatic organisms