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EC number: 228-705-0 | CAS number: 6330-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well reported non guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
- Principles of method if other than guideline:
- Single ip injection with 15 day observation period in mice
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 2-cyano-N-methylacetamide
- EC Number:
- 228-705-0
- EC Name:
- 2-cyano-N-methylacetamide
- Cas Number:
- 6330-25-2
- Molecular formula:
- C4H6N2O
- IUPAC Name:
- 2-cyano-N-methylacetamide
- Details on test material:
- - Name of test material: N-Methylcyanacetamid
- Physical state: powder
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Wilhelm Gassner, Sulzfeld
- Age at study initiation: about 4 weeks
- Weight at study initiation: males, 700 mg/kg: 26.0 g; males, 200 mg/kg: 28.0 g; females, 700 mg/kg: 24.0 g; females, 200 mg/kg: 28.0 g
- Fasting period before study: 16 h (water ad libitum)
- Housing: Makrolon cages, Typ I
- Diet (ad libitum): Herilan, MRH-Haltung; H. Eggersmann KG, Rinteln
- Water (ad libitum): VE water on working days, tap water on holidays
- Acclimation period: At least 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): 45 - 75
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
- Doses:
- 200 and 700 mg/kg
- No. of animals per sex per dose:
- 5 animals per sex per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations and weighing: less than 15 min, 15 min, 30 min, 1 h, 2 h, 4 h and 5 h after application. Afterwards once per day.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and body weight
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 200 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 279 mg/kg bw
- Based on:
- test mat.
- Mortality:
- After a single application of 700 mg/kg all mice (male and female) were dead after 1 day. Three males and one female died 1 day after the application of 200 mg/kg test substance.
- Clinical signs:
- After 15 min the first clinical signs were observed at both dose levels, including dyspnea, apathy, staggering, convulsions, ruffled haircoat, hypohydration and (a general) bad condition. Additionally, an application of 200 mg/kg resulted in twitching, clonic cramps and spastic gaits of the animals. Petrusions of the eyeballs were observed at the dose level of 700 mg/kg only
- Body weight:
- 200 mg/kg: The males gained body weight during the test, whereas the females has an almost stable weight.
700 mg/kg: Weighting was only performed at the beginning of the experiment, since all animals were dead after 1 day. - Gross pathology:
- Neither precipitation of the substance nor adhesions were found in the abdomen of dead and sacrified animals.
Any other information on results incl. tables
Table 1: acute toxicity after intraperitonial application
Table 2: Mortality and body weight
Table 3: Acute toxicity - males
Table 4: Acute toxicity - females
Table 5: Acute toxicity - male and female together
|
Applicant's summary and conclusion
- Executive summary:
The acute toxicity of 2 -cyano-N-methylacetamide via the intraperitonial route was investigated in male and female mice. Doses of 200 mg/kg bw and 700 mg/kw bw were applied to 5 animals per sex per dose. After a single application of the test substance main symptoms of intoxication were dyspnea, apathy, abnormal body positions, staggering, tonic cramps, ruffled haircoat, hypohydration and a general bad condition of the animals, starting at the lowest dose level partially already 15 min after application. After 15 min the first clinical signs were observed at both dose levels, including dyspnea, apathy, staggering, convulsions, ruffled haircoat, hypohydration and (a general) bad condition. Additionally, an application of 200 mg/kg resulted in twitching, clonic cramps and spastic gaits of the animals. Petrusions of the eyeballs were observed at the dose level of 700 mg/kg only. After the application of 700 mg/kg all mice (male and female) were dead after 1 day. Three males and one female died 1 day after the application of 200 mg/kg test substance. Neither precipitation of the substance nor adhesions were found in the abdomen of dead and sacrified animals. The LD50 value for both, male and female together was found at 200 mg/kg bw.
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