Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 946-342-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 June 2000 - 29 June 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- A limit test was conducted at saturated test item concentration. Test concentrations were analysed by TOC analysis.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: Control and 3.5 mg/L (saturated)
- Sampling method: Samples collected at the test start and after 48 hours of exposure - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution of 30 mg/L was prepared by weighing the test substance into water and treating for 60 seconds at 8000 rpm with an ultra turrax and then stirring for 24 hours on a magnetic stirrer. The emulsion was then filtered using a folded filter (pore size 7 to12 µm).
- Controls: M4-medium according to Elendt and BGA (1992) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: STRAUS, parthenogenetic females
- Source: Laboratory-bred
- Age at study initiation: 0-24 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 15.4 °dH
- Test temperature:
- 20.3 and 20.8 °C at 48 hours
- pH:
- 7.8 and 7.9 at 48 hours
- Dissolved oxygen:
- 8.7 and 8.9 mg/L at 48 hours
- Nominal and measured concentrations:
- Nominal concentration: 3.5 mg/L
Arithmetic mean measured concentration: 3.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Cylindrical test vessels, diameter 4.0 cm, height 6.5 cm, not ventilated
- Fill volume: 20 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
EFFECT PARAMETERS MEASURED: Number of immobile daphnids recorded after 24 and 48 hours. Oxygen, pH and water temperature were determined from the total volume of the parallel solutions at 48 hours. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- 15% immobilsation rate at 3.9 mg/L
- Duration:
- 48 h
- Dose descriptor:
- other: EC15
- Effect conc.:
- 3.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity criteria of the test guideline permit a maximum immobilisation rate of 10% for control daphnia (actual: 0%).
- Conclusions:
- The 48 h EC50 for the test item, based on immobilisation of Daphnia magna, was >3.9 mg/L. 15% immobilisation was determined at a measured concentration of 3.9 mg/L.
- Executive summary:
A limit test was performed to assess the acute toxicity of the test item to Daphnia magna over 48 hours in a static system. A stock solution of 30 mg/L test item was prepared by weighing the test substance into water and treating for 60 seconds with an ultra turrax and then stirring for 24 hours on a magnetic stirrer. The emulsion was then filtered. The limit test was conducted at a nominal concentration of 3.5 mg/L (arithmetic mean measured concentration 3.9 mg/L, based on TOC analysis) alongside a control. The 48 h EC50 based on immobilisation was determined to be >3.9 mg/L. 15% immobilisation was determined at a measured concentration of 3.9 mg/L. This study is GLP-compliant and was performed according to EU Method C.2. This study is considered reliable with restrictions (Klimisch 2) as there were limitations in reporting and test concentrations were analysed using a non-(substance)-specific analytical technique.
Reference
Table 1. Immobilisation results
Arithmetic mean measured concentrations (mg/L) | Cumulative immobilised daphnids (n=20) | % immobilised daphnids (n=20) | ||
24 hours | 48 hours | 24 hours | 48 hours | |
Control | 0 | 0 | 0 | 0 |
3.9 | 0 | 3 | 0 | 15 |
Description of key information
The 48 h EC50 for the test item, based on immobilisation of Daphnia magna, was > 3.9 mg/L. 15% immobilisation was determined at a measured concentration of 3.9 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.9 ng/L
Additional information
A limit test was performed to assess the acute toxicity of the test item to Daphnia magna over 48 hours in a static system. A stock solution of 30 mg/L test item was prepared by weighing the test substance into water and treating for 60 seconds with an ultra turrax and then stirring for 24 hours on a magnetic stirrer. The emulsion was then filtered. The limit test was conducted at a nominal concentration of 3.5 mg/L (arithmetic mean measured concentration 3.9 mg/L, based on TOC analysis) alongside a control. The 48 h EC50 based on immobilisation was determined to be >3.9 mg/L. 15% immobilisation was determined at a measured concentration of 3.9 mg/L. This study is GLP-compliant and was performed according to EU Method C.2. This study is considered reliable with restrictions (Klimisch 2) as there were limitations in reporting and test concentrations were analysed using a non-(substance)-specific analytical technique.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.