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EC number: 420-150-4 | CAS number: 80498-15-3 SP 807
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 January 1996 -15 April 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate included in study report
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg dry material per litre
- Sampling method: Duplicate samples from the control and the test solutions of SP807 were taken at 72h (newly prepared solution) and at 96h (spent solution after 24h). Samples of ca. 5 mL were taken in polyethylene bottles.
- Sample storage conditions before analysis: frozen - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 50 mL of the test substance was transferred to one aquaria, containing 30 litres of dilution water (DSWL), resulting in a test solution of 100 mg dry material per litre corresponding with a specific activity of 406 LACU per litre.
- Controls: Dilution water (DSWL) - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Keijzersberg in Blitterswijk, The Netherlands
- Length at study initiation (length definition, mean, range and SD): 5.3 +/-0.3 cm.
- Weight at study initiation (mean and range, SD): 3.08 +/- 1.13 g
- Feeding during test: no feeding. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 205 mg CaCO3/L
- Test temperature:
- 14.8°C - 15.1°C
- pH:
- 7.7-8.0
- Dissolved oxygen:
- 8.4 mg/L - 9.9 mg/L
- Nominal and measured concentrations:
- t=72h: the measured concentration was 64-67% of the nominal value
t=96h: the measured concentration was 79-81% of the nominal value. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquarium
- Material, size, headspace, fill volume: glass, 46 litres containing 30 litre of control or test medium
- Aeration: yes
- Renewal rate of test solution (frequency): 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- Biomass loading rate: 1g per litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Addition of several salts to ground water from a locality near Linschoten (The Netherlands)
- Total organic carbon: 1.7 mg/L
- Metals: << 1mg/L
- Chlorine: 2.76 mmol/L
- Alkalinity: 1.39 mmol HCO3-/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light/8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After 3h, 25h, 48h, 72h and 96h dead and living animals were counted. At the same time the condition (swimming behaviour, colour or any other visually observable morphological or behavioural criterion) of the survivors was compared with that of the control animals.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: dry material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 73 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: dry material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: dry material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 73 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: dry material
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: dry material
- Basis for effect:
- other: condition
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 73 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: dry material
- Basis for effect:
- other: condition
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Remarks:
- active enzyme protein
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 10%
- Validity criteria fulfilled:
- not specified
- Conclusions:
- No mortality was observed following the exposure of Oncorhynchus mykiss to the test substance at a concentration of 73 mg dry material/L. Due to the low toxicity of the test material, the 96-hour LC50 value of the substance was unable to be calculated. Therefore the following values were assumed:
The 96-hour LC50 value for the test substance with rainbow trout was determined to be >73 mg dry material/L corresponding to 2.64 > mg active enzyme protein (aep)/L.
The no-observed effect concentration (NOEC) was 73 mg dry material per litre corresponding to 2.64 mg aep/L. - Executive summary:
An acute toxicity test to rainbow trout (Oncorhynchus mykiss) was conducted to determine the effect of laccase on fish. The study was conducted in accordance with OECD and EC guidelines, and in compliance with GLP.
The test substance was not acutely toxic to rainbow trout as no mortality was observed during the 96 hour exposure period.
Test concentrations are based on dry material. Verification of test concentrations was performed based on enzyme activity. The amount of measured enzyme activity was then converted to mg dry material/L in order to calculate the achieved measured concentrations.
No mortalities were observed in the test group and therefore the 96-hour LC50 was considered greater than the maximum concentration tested. The following results are expressed in terms of the mean measured concentration:
96-hour LC50: > 73 mg dry material/L or >2.6 mg active enzyme protein (aep)/L
Highest concentration resulting in 0% mortality: 73 mg dry material/L or > 2.6 mg aep/L
Lowest concentration resulting in 100% mortality: >73 mg dry material/L or > 2.6 mg aep/L
No-observed effect concentration (NOEC): 73 mg dry material/L or > 2.6 mg aep/L
Reference
Description of key information
No mortality was observed following the exposure of Oncorhynchus mykiss to the test substance at a concentration of 73 mg dry material/L. Due to the low toxicity of the test material, the 96-hour LC50 value of the substance was unable to be calculated. Therefore the following values were assumed: The 96-hour LC50 value for the test substance with rainbow trout was determined to be >73 mg dry material/L corresponding to > 2.6 mg aep/L. The no-observed effect concentration (NOEC) was 73 mg dry material per litre corresponding to 2.6 mg aep/l.
Key value for chemical safety assessment
Additional information
An acute toxicity test to rainbow trout (Oncorhynchus mykiss) was conducted to determine the effect of laccase on fish. The study was conducted in accordance with OECD and EC guidelines, and in compliance with GLP.
The test substance was not acutely toxic to rainbow trout as no mortality was observed during the 96 hour exposure period.
Test concentrations are based on dry material. Verification of test concentrations was performed based on enzyme activity. The amount of measured enzyme activity was then converted to mg dry material/L in order to calculate the achieved measured concentrations.
No mortalities were observed in the test group and therefore the 96-hour LC50 was considered greater than the maximum concentration tested. The following results are expressed in terms of the mean measured concentration:
96-hour LC50: > 73 mg dry material/L or >2.6 mg aep/L
Highest concentration resulting in 0% mortality: 73 mg dry material/L or > 2.6 mg aep/L
Lowest concentration resulting in 100% mortality: >73 mg dry material/L or > 2.6 mg aep/L
No-observed effect concentration (NOEC): 73 mg dry material/L or 2.6 mg aep/L
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