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EC number: 283-810-9 | CAS number: 84713-17-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-10-08 to 1985-10-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- (1981)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,2,4(or 2,4,4)-trimethylhexanedinitrile
- EC Number:
- 283-810-9
- EC Name:
- 2,2,4(or 2,4,4)-trimethylhexanedinitrile
- Cas Number:
- 84713-17-7
- Molecular formula:
- C9H14N2
- IUPAC Name:
- 2,2,4-trimethylhexanedinitrile; 2,4,4-trimethylhexanedinitrile
- Details on test material:
- 2,2,4(or 2,4,4)-Trimethylhexanedinitrile of Hüls AG, purity 96.5 %, main impurity 1-cyano-2-imino-3,5,5-trimethylcyclopentane = 2.42 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ORGANISMS:
- Strain: Bor: WISW (SPF TNO)
- Source: F. Winkelmann, Borchen (Germany)
- Weight at study initiation: 5 males mean 124 g, 5 females mean 110 g
- Fasting period before study: 16 hours
- Diet: ad libitum, R10 special diet for rats, SSniff R
- Water: ad libitum, tap water
- Acclimation period: 4 - 8 days
ENVIRONMENTAL CONDITIONS
- Temperature: 20 +/- 1 °C
- Humidity: 60 +/- 5 %
- Photoperiod: 12 hours artificial light, 12 hours dark
- Air changes: 15 per hour
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- ADMINISTRATION:
- Doses per time period: single dose (gavage)
- Volume administered or concentration: 0.273-0.432 ml/kg bw, undiluted
- Post dose observation period: 14 days - Doses:
- 251; 283.5; 316; 398 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- EXAMINATIONS:
- Body weights: before, and 1, 7, 14 days post dosing
- Clinical signs and mortality: within 6 hours after dosing, thereafter daily
- Necropsy: all animals (macroscopic) - Statistics:
- LD50 is generally determined according to Litchfield and Wilcoxon, reported with 95 % confidence limits.
Means of body weights were calcultated
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 290 mg/kg bw
- 95% CL:
- >= 254 - <= 331
- Mortality:
- - Number of deaths at each dose:
251 mg/kg bw: 2 males, 0 females dead within 80 minutes
283.5 mg/kg bw: 3 males, 2 females dead within 90 minutes
316 mg/kg bw: 5 males, 2 females dead within 105 minutes
398 mg/kg bw: 4 males, 4 females dead within 135 minutes - Clinical signs:
- - 3 to 7 minutes after dosing: Tonic and clonic convulsions with vocalization and marked opisthotonos and exophthalmos, salivation, bloody
eyes and noses, lateral or prone position, heavy breathing
- 24 hours after dosing: Surviving animals were symptom-free. - Body weight:
- Body weight gain was not affected.
- Gross pathology:
- - animals that died during the study: Hyperemia of the small intestinal mucosa, and hyperemia and swelling of the mucosa of the stomach
- terminal sacrifice: Only one animal showed hyperemia of the ileum. - Other findings:
- no other findings
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: other: EU Directive 67/548 EEC
- Conclusions:
- According to this study the LD50 value (oral) was determined to be 290 mg/kg bw in rats for 2,2,4(or 2,4,4)-trimethylhexanedinitrile. Thus the substance is considered to be harmful.
- Executive summary:
- The test item 2,2,4(or 2,4,4)-trimethylhexanedinitrile was applied once to 4 dose-groups of rats (5 male and 5 female Wistar rats per dose-group) in doses of 251; 283.5; 316; 398 mg/kg bw as suspension in water. The observation period was 14 days. Animals died within 80 - 135 minutes after oral application of the test item. Clinical signs started 3 to 7 minutes after application and were discribed as tonic and clonic convulsions with vocalization and marked opisthotonos and exophthalmos, salivation, bloody eyes and noses, lateral or prone position, heavy breathing. Surviving animals were symptom-free 24 hours after dosing. Animals that died during the study showed hyperemia of the small intestinal mucosa and hyperemia and swelling of the mucosa of the stomach. Dissection at the end of the experiment revealed only one animal with hyperemia of the ileum. According to this study the LD50 value (oral) was determined to be 290 (254 - 331) mg/kg bw in rats for the test item 2,2,4(or 2,4,4)-trimethylhexanedinitrile.
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