2-methyl-2-nitropropan-1-ol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPP 81-2 (Acute Dermal Toxicity)

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-2-nitropropan-1-ol

EC Number:

200-957-6

EC Name:

2-methyl-2-nitropropan-1-ol

Cas Number:

76-39-1

Molecular formula:

C4H9NO3

IUPAC Name:

2-methyl-2-nitropropan-1-ol

Details on test material:

Purity is above 99%.

Specific details on test material used for the study:

NMP Crystals, 2-nitro-2-methyl-1-propanol, 99.6% purity

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

Test was conduced using 10 rabbits (5 male; 5 female) weighing 2.9 +/- 0.5 kg. The abdomens of the rabbits were shaved free of hair and the skin in one group of rabbits was further prepared by abrasion.The abrasions were made with a blunt hypodermic needle without obtaining bleeding and were 2-3 cm apart over the area of exposure.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The abdomens of the rabbits were shaved free of hair and the skin in one group of rabbits was further prepared by abrasion.The abrasions were made with a blunt hypodermic needle without obtaining bleeding and were 2-3 cm apart over the area of exposure. Test material was ground to a fine powder. For each rabbit a weight amount of test material to give a dose of 2000 mg/kg was spread over the prepared abdominal are. The skin area was then covered with gauze and a sheet of impervious rubberized cloth to prevent loss of test material. The trunk was further enclosed with a flexible wire screen help in place by tape. Animals were then returned to their cages. After 24 hours the bindings and patches were removed and the exposed skin areas gently cleaned and observed for skin irritancy. The animals wre continued to be oberved for another 14 days.

Duration of exposure:

24 hours

Doses:

One dose: 2000 mg/kg

No. of animals per sex per dose:

10 (5 males' 5 females)

Control animals:

no

Details on study design:

For each rabbit a weight amount of test material to give a dose of 2000 mg/kg was spread over the prepared abdominal are. The skin area was then covered with gauze and a sheet of impervious rubberized cloth to prevent loss of test material. The trunk was further enclosed with a flexible wire screen help in place by tape. Animals were then returned to their cages. After 24 hours the bindings and patches were removed and the exposed skin areas gently cleaned and observed for skin irritancy. The animals wre continued to be oberved for another 14 days for any gross signs of toxicity. At the end of the 14 day observation period the animals were weighed, sacrificed and the organs examined for gross pathology

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

other: Limit test

Remarks:

No signs of toxicity or abnormal behavior. At necropsy organs in all animals were grossly normal

Effect level:

2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality

Clinical signs:

None

Body weight:

No significant change

Gross pathology:

No findings