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EC number: 221-717-7 | CAS number: 3209-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404. No signs for skin irritation were observed throughout the test and observation period.
The eye irritation potential of 1,2-dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405. 1,2-dichloro-3 -nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- adopted in 1981
- Deviations:
- yes
- Remarks:
- 8 d observation period
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- semiocclusive
- Preparation of test site:
- other: clipped skin
- Vehicle:
- other: oil (not further specified)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 mg - Duration of treatment / exposure:
- 4 h
- Observation period:
- 1, 24, 48 and 72 h, and 8 days
- Number of animals:
- 3
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 0
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 48 h
- Score:
- 0
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 72 h
- Score:
- 0
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 0
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 48 h
- Score:
- 0
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 72 h
- Score:
- 0
- Interpretation of results:
- GHS criteria not met
- Executive summary:
Schreiber G (1982)
The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404 (adopted: 12 May 1981). 0.5 g 1,2 -dichloro-3 -nitrobenzene ground in oil was applied to the clipped left flank of each of 3 New Zealand White rabbits for 4 h under semi-occlusive conditions. Thereafter patches and test material were removed. The test sites were examined and scored for erythema and edema using the Draize method 1, 24, 48 and 72 h as well as 8 days after exposure. The mean erythema and edema scores over 24, 48 and 72 h were 0.0 for both end points in all animals. No signs for skin irritation were observed throughout the test and observation period.
Reference
Erythema/edema scores
|
Time point after patch removal |
Animal No. |
Sum |
Average |
||
1 |
2 |
3 |
|
|
||
Erythema score |
1 h |
0 |
0 |
0 |
|
|
24 h |
0 |
0 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
0 |
|
8 days |
0 |
0 |
0 |
|
|
|
Edema score |
1 h |
0 |
0 |
0 |
|
|
24 h |
0 |
0 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
0 |
|
8 days |
0 |
0 |
0 |
|
|
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- adopted in 1981
- Deviations:
- yes
- Remarks:
- observation period 8 d
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12 - Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µl bulk volume - Duration of treatment / exposure:
- continuous
- Observation period (in vivo):
- 1, 24, 48, and 72 h, and 8 days
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- TOOL USED TO ASSESS SCORE: fluorescein
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at 24, 48 and 72 hrs
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: score = 0 at 24, 48 and 72 hrs
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: score = 0 at 24, 48 and 72 hrs
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at 24, 48 and 72 hrs
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- mean
- Time point:
- other: 1 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Remarks on result:
- other: fully reversible
- Irritation parameter:
- conjunctivae score
- Remarks:
- swelling
- Basis:
- mean
- Time point:
- other: 1 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Remarks on result:
- other: fully reversible
- Irritation parameter:
- conjunctivae score
- Remarks:
- efflux
- Basis:
- mean
- Time point:
- other: 1 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Remarks on result:
- other: fully reversible
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 1, 24, 48 and 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no effects
- Remarks on result:
- other: no effects
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 1, 24, 48 and 72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: no effects
- Remarks on result:
- other: no effects
- Executive summary:
The eye irritation potential of 1,2-dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405 (adopted: 12 May 1981). A bulk volume of 0.1 mL of the crystalline test substance was applied into the conjunctival sac of the right eye of each animal, the left eye serving as control. The eyes were not rinsed after administration of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 24, 48 and 72 h as well as 8 days after application. 1,2-dichloro-3-nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals (Schreiber, 1982).
Reference
No signs of eye irritation according to cornea and iris scores were observed throughout the test and observation period. One hour after application of the test substance, all animals showed slight conjunctival redness and swelling, as well as moderate efflux. These effects were fully reversible within 24 h.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Additional information
The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404 (adopted: 12 May 1981). 0.5 g 1,2 -dichloro-3-nitrobenzene ground in oil was applied to the clipped left flank of each of 3 New Zealand White rabbits for 4 h under semi-occlusive conditions. Thereafter patches and test material were removed. The test sites were examined and scored for erythema and edema using the Draize method 1, 24, 48 and 72 h as well as 8 days after exposure. The mean erythema and edema scores over 24, 48 and 72 h were 0.0 for both end points in all animals. No signs for skin irritation were observed throughout the test and observation period (Schreiber 1982).
The eye irritation potential of 1,2 -dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405 (adopted: 12 May 1981). A bulk volume of 0.1 mL of the crystalline test substance was applied into the conjunctival sac of the right eye of each animal, the left eye serving as control. The eyes were not rinsed after administration of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 24, 48 and 72 h as well as 8 days after application. 1,2-dichloro-3-nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals (Schreiber 1982).
Justification for classification or non-classification
1,2-dichloro-3-nitrobenzene is not irritatin to skin and eyes. According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification is not justified.
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