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EC number: 225-876-3 | CAS number: 5131-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- calculation (if not (Q)SAR)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- accepted calculation method
- Justification for type of information:
- Data is from computational model developed by USEPA
- Qualifier:
- according to guideline
- Guideline:
- other: Modelling database
- Principles of method if other than guideline:
- Prediction done using ECOSAR v.1.11 model
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-nitrobenzene-1,3-diamine
- Molecular formula (if other than submission substance): C6H7N3O2
- Molecular weight (if other than submission substance): 153.14 g/mol
- Smiles notation (if other than submission substance): c1(c(cc(N)cc1)N)[N+](=O)[O-]
- InChI: 1S/C6H7N3O2/c7-4-1-2-6(9(10)11)5(8)3-4/h1-3H,7-8H2
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Fish
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Test temperature:
- 25 Deg.C
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 515.31 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: ECOSAR Class:Neutral Organics
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the EPI Suite ECOSAR version 1.10 prediction model, the 96 hours LC50 was estimated to be 2515.31 mg/l on Fish for substance 4-nitrobenzene-1,3-diamine (CAS no.5131-58-8) on the basis of mortality effects.
- Executive summary:
Using the EPI Suite ECOSAR version 1.10, the short term toxicity on fish was predicted for test substance 4 -nitrobenzene-1,3 -diamine (CAS: 5131 -58 -8). On the basis of effects observed in a static freshwater system, the lethal concentration LC50 value for the substance is estimated to be 2515.31 mg/l for fish for 96 hr duration. Based on this value, it can be concluded that the test chemical 4 -nitrobenzene-1,3 -diamine can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.
Reference
Description of key information
Using the EPI Suite ECOSAR version 1.10, the short term toxicity on fish was predicted for test substance 4 -nitrobenzene-1,3 -diamine (CAS: 5131 -58 -8). On the basis of effects observed in a static freshwater system, the lethal concentration LC50 value for the substance is estimated to be 2515.31 mg/l for fish for 96 hr duration. Based on this value, it can be concluded that the test chemical 4 -nitrobenzene-1,3 -diamine can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2 515.31 mg/L
Additional information
Three studies including predicted data from validated model and experimental studies from authorative database for short term fish toxicity endpoint of target chemical 4-nitrobenzene-1, 3-diamine (Cas no. 5131-58-8) with relevant read across chemical which is structurally similar to target were summarized as follows:
First prediction using ECOSAR v.1.11 model which indicate the short term toxicity on fish for test substance 4 -nitrobenzene-1,3 -diamine (CAS: 5131 -58 -8). On the basis of effects observed in a static freshwater system, the lethal concentration LC50 value for the substance is estimated to be 2515.31 mg/l for fish for 96 hr duration. Based on this value, it can be concluded that the test chemical 4 -nitrobenzene-1,3 -diamine can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.
Above aquatic classification supported by experiment data of read across chemical 4-nitroaniline (Cas no. 100-01-6) suggest in Acute fish toxicity data the lethal concentration (LC50) determine of test substance 4-nitroaniline for exposure period of 96 hrs. on the basis of mortality effects. Test organism was used asPimephales promelas (Fathead Minnow)in static freshwater with test temp.22 deg.C and dissolved oxygen > 40 %,pH 7.2 to 7.6,Hardness 40 to 48 mg/L CaCO3 and ALKALINITY:30 to 35 mg/L CaCO3 with conductivity 120-160 UMHOS/CM.The Lethal concentration to 50% of test organisms(LC50) on the basis of mortality effect was observed to be 101.8 mg/l for exposure period of 96 hrs.(Ecotox database by US EPA)
Last study from same data source i.e Ecotox database for same read across chemical 4-nitroaniline (Cas no. 100-01-6) suggest the lethal concentration (LC50) determine of test substance 4-nitroaniline for exposure period of 96 hrs. on the basis of mortality effects. Test organism was used as Pimephales promelas (Fathead Minnow) of 32 days age with 20.3 MM length and 0.107 G weight in static freshwater with test temp.24.9deg.C and dissolved oxygen6.3 mg/l,pH7.5,Hardness48.3mg/L CaCO3 and ALKALINITY: 43.5mg/L CaCO3.The Lethal concentration to 50% of test organisms (LC50) on the basis of mortality effect was observed to be 125 mg/l for exposure period of 96 hrs with 95% confidence limit 110-142.
Thus all above available studies for short term fish toxicity give common conclusion as test chemical 4-nitrobenzene-1, 3-diamine (Cas no. 5131-58-8) was non hazardous to fish as per the CLP regulation since the lethal concentration (LC50) is in the range 101.8 -2515.31 mg/l.
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