4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21-11-2017 - 21-11-2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

no

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

Test Item Name : 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride
CAS No. : 6358-36-7
Batch No./ Lot no. : KCP/FS/44/17
Physical Appearance : Yellow powder
Light sensitive / Hygroscopic / Volatile / None applicable: -
Date of manufacturing : 2017-05-01
Date of expiry : 2017-04-12
Purity : not avaiable in COA
Stability : Stable in recommended storage conditions
Storage Conditions : 1. Ambient Temp (23 to 27 °C) (Keep container tightly closed in a dry & wellventilated place. Store in cool place. Do not expose to air, light & moisture.)
Precautions : Wear respiratory protection. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation.
Evacuate personnel to safe areas. Avoid breathing dust.

Radiolabelling:

not specified

Test temperature:

25°C

Details on study design: HPLC method:

HPLC Procedure:
The solutions of the test substance and reference substances were prepared in solvent mentioned in 4.3. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor.

Operating Conditions:
HPLC Model No. : Agilent Technologies,1260 Infinity
Flow Rate : 1.0ml/min
Column : ZORBAX Eclipse Plus C18, 4.6×100 mm,3.5 μm
Mobile phase : Methanol: water (55:45)
Temperature : 25°C
Detector : Diode Array Detector @210 nm
Injector : Autosampler
Injector volume : 10μl
Data Acquisition software : Chem Station Open lab Control Panel

Key result

Type:

log Koc

Value:

1.809 dimensionless

pH:

7

Temp.:

25 °C

Remarks on result:

other: other details not specified

Details on results (HPLC method):

The Adsorption coefficient (KOC) on soil and on sewage sludge of the 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride (CAS No. 6358-36-7) was found to be 1.809 ± 0.001 at 25°C

Test substance4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride		K	Log k	Log Koc by Generalized Calibration Graph	Mean of Log Koc ± S.D.
tR1	1.913	0.976	-0.010	1.808	1.809± 0.001
tR2	1.915	0.978	-0.010	1.809

Validity criteria fulfilled:

yes

Conclusions:

The Adsorption Coefficient of test substance 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride (CAS No. 6358-36-7) was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was found to be 1.809 ± 0.001 at 25°C.

Executive summary:

The adsorption coefficient Koc in soil and in sewage sludge 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride(CAS No.6358-36-7) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference

substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were N-methylaniline, 2 - nitrophenol, Nitrobenzene, 4-Nitrobenzamide, N,N-dimethylbenzamide, N-methylbenzamide, Benzamide having Koc value ranging from 1.239 to 2.47 The Log Koc value was found to be 1.809 ± 0.001at 25°C.

This value indicates that the test substance has low sorption to soil and sediment, moderate migration to ground water.

Description of key information

The Adsorption Coefficient of test substance 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride (CAS No. 6358-36-7) was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was found to be 1.809 ± 0.001 at 25°C.

Key value for chemical safety assessment

Koc at 20 °C:

64.41

Additional information

The adsorption coefficient Koc in soil and in sewage sludge 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride(CAS No.6358-36-7) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference

substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were N-methylaniline, 2 - nitrophenol, Nitrobenzene, 4-Nitrobenzamide, N,N-dimethylbenzamide, N-methylbenzamide, Benzamide having Koc value ranging from 1.239 to 2.47 The Log Koc value was found to be 1.809 ± 0.001at 25°C.

This value indicates that the test substance has low sorption to soil and sediment, moderate migration to ground water.

[LogKoc: 1.809]