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Diss Factsheets
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EC number: 239-555-0 | CAS number: 15520-05-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,2'-(octylimino)bisethanol
- EC Number:
- 239-555-0
- EC Name:
- 2,2'-(octylimino)bisethanol
- Cas Number:
- 15520-05-5
- Molecular formula:
- C12H27NO2
- IUPAC Name:
- 2-[(2-hydroxyethyl)(octyl)amino]ethan-1-ol
- Test material form:
- other: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- origin of test animals: HOECHST AG, Kastengrund, SPF-breeding
details on species: Wistar-rat / Hoe: WISKf (SPF71)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Doses:
- test animals female: 800/1250/1600/2000 mg/kg bw
test animals male: 2000 mg/kg bw - No. of animals per sex per dose:
- test animals female: 5 animal per dose (800/1250/1600/2000 mg/kg bw)
test animals male: 5 animal per dose (2000 mg/kg bw) - Control animals:
- no
- Details on study design:
- The test item in sesam oil was admistered by gavage in the above mentioned doses. The observation periode after the application was 14 days. The apperance of symthoms and lethality was registered twice a day (forenoon and afternoon), once a day on week-end and on public holidays. During this periode of time the weight of the animals was recorded. The number of animals died during the test peride were anatomized and examined for macroscopic findings..
The number of surviving animals were sacrificed at the end of the observation periode by carbon monoxide. These testing animals were also anatomized and examined for macroscopic findings. - Statistics:
- The LD50, the 95% confidence level as well as the equation of the probit straight line were calculated for the femal testing animals by the ratio of lethality via the probit analysis. (Methode according FIELLER and SIDAK, softwar developement: Pharma Forschung and
Entwicklung Informatik der Hoechst AG)
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 157 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 122 - <= 1 736
- Mortality:
- male test animals: 3/5 (2000 mg/kg bw dose group)
female test animals: 1/5 (800 mg/kg bw dose group); 3/5 (1250 mg/kg bw dose group); 4/5 (1600 mg/kg bw dose group); 4/5 (2000 mg/kg bw dose group) - Clinical signs:
- other: 1157 mg/kg bwObserved by female and male test animals after the application of the test item: 1) reduced spontaneous activity 2) daze feeling 3) crouch 4) long-legged walk 5) uncoordinated/tumbled walk 6) face-down position 7) narrowed palpebral fissure
- Gross pathology:
- The macroscopic examination of the male/female test animals died during the testing showed ablation of the mucous membrane of the gastrointestinal tract. Furthermore, the small intestine of the female test animals died during the testing was partial reddened and at one female test animal of the 2000 mg/kg bw dose group it was filled with a red, mucous material (blood).
Those animals killed after the observation periode showed no visible macroscopic finding.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- LD50 (oral, female rat): 1157 mg/kg body weight
- Executive summary:
In order to assess the acute oral toxicity of the test item, an OECD 401 study in rats was performed. Mortality occurred in the male group in 3/5 animals. In the female group mortality occurred in 1/5, 3/5, 4/5 and 4/5 animals of the 800, 1250, 1600 and 2000 mg/kg body weight dose groups, respectively. At higher doses unspecific findings like irregular respiration, irregular movements, narrow palpebral fissure, mydriasis and prone position were observed. All clinical signs were reversible within 2 days after treatment. Body weight development during the course of the study was not affected by the treatment. Necropsy of deceased animals: Partial delamination of gastric lining, reddening of small intestine. All surviving animals which were sacrificed at the end of the 14-day observation period were free of any macroscopic findings.
The median lethal dose of the test substance after single oral administration to male and female rats, observed over a period of 14 days is determined to be: LD50(oral, female rat): 1157 mg/kg body weight .
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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