Methyl carbamate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From April 14, 1986 to April 16, 1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to method given by U. S. Environmental Protection Agency (1975) and American Public Health Association (1980), in compliance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: U. S. Environmental Protection Agency (1975). Methods of acute toxicity tests with fish, macroinvertebrates and amphibians

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: American Public Health Association (1980). Standard methods for examination of water and wastewater

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl carbamate

EC Number:

209-939-2

EC Name:

Methyl carbamate

Cas Number:

598-55-0

Molecular formula:

C2H5NO2

IUPAC Name:

methyl carbamate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna
- Source: ABC facilities
- Food type: The adult Daphnia were fed algae (Selenastrum capricornutum) at least every 3 d prior to testing and supplemented with a suspension of fish food.

ACCLIMATIZATION
- Acclimatization period: 3 d

Study design

Test type:

static

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

225-275 ppm

Test temperature:

20°C

pH:

8.4

Dissolved oxygen:

7.0 - 9.2 mg/L

Nominal and measured concentrations:

0, 100, 180, 320, 560 and 1,000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250-mL glass beakers containing 200 mL of ABC aged well water.
- No. of organisms per vessel: 10 per beaker
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 325-375 ppm
- Conductivity: 700 μmhos/cm
- Aluminum: <20 ppb
- Arsenic: <0.2 ppb
- Cadmium: <2 ppb
- Chromium: <3 ppb
- Cobalt: <4 ppb
- Copper: <3 ppb
- Iron: 12 ppb
- Lead: <5 ppb
-Mercury: <0.5 ppb
- Nickel: <15 ppb
- Silver: <5 ppb
- Zinc: 11 ppb
- Measured organophosphorus pesticides: Less than minimum detectable limit (<0.10 ppb)
- Measured organochlorine pesticides plus PCB's: Less than minimum detectable limit (<0.50 ppb)
- Intervals of water quality measurement: 0 and 48 h

OTHER TEST CONDITIONS
- Photoperiod: 16 h daylight photoperiod, with 30 min simulated dawn and dusk periods
- Light intensity: 50-70 foot candles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mortality and behavioral observations at 24 h and 48 h.

TEST CONCENTRATIONS
- Range finding study: 10 Daphnia each in exposure concentrations of 1.0, 10 and 100 mg/L. From this information, concentration were selected for the definitive bioassay
- Test concentrations for definitive study: 0, 100, 180, 320, 560 and 1,000 mg/L
- Spacing factor for concentration: Geometric series in which the ratio between consecutive concentrations is between 1.5 and 2.0.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Lack of mortality and abnormal effects

Details on results:

No abnormal effects such as mortality, surfacing or Daphnids lying on the bottom of test vessels were observed in any of the test concentrations during the test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24 h and 48 h LC50 for the test substance was determined to be >1,000 mg/L and NOEC was determined to be 1,000 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna under static conditions, according to method given by U. S. Environmental Protection Agency (1975) and American Public Health Association (1980), in compliance with GLP. Based on the findings of a range finding test, ten Daphnia (less than 24 h old) per beaker were exposed to test substance concentration of 0, 100, 180, 320, 560 and 1,000 mg/L in duplicate for 48 h. Water quality parameters of temperature, dissolved oxygen and pH were measured at 0 and 48 h interval and they were found to be within acceptable limits. No abnormal effects such as mortality, surfacing or Daphnids lying on the bottom of test vessels were observed in any of the test concentrations during the test. Hence based on the findings of the study, the 24 h and 48 h LC₅₀ for the test substance was determined to be >1,000 mg/L and NOEC was determined to be 1,000 mg/L (Forbis AD, 1986).