Sodium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxybenzene-1-sulphonamidato(2-)]chromate(1-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5 screening test

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

August 5, 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: Ciba Geigy internal method A-15

Deviations:

not specified

Principles of method if other than guideline:

The biological oxygen demand BOD is the amount of oxygen needed to degrade organic substances present in waste water with the help of bacteria (and other microorganisms present in sludge). The expression BOD5 represents the oxygen consumption within 5 days of incubation at 20 °C. Various dilutions of a substance solution in oxygen saturated water will be measured for its oxygen content before and after such an incubation period.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

not specified

Key result

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

ca. 0 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 0 mg O2/g test mat.

From each sample three BOD-vessels are prepared in parallel. Two vessels are incubated for 5 days in the incubator, the third one is used to measure the start-value. This vessel is immediately added with 0.5 ml MnCl2 (60 % solution) and 0.5 ml NaOH (30 % solution) and closed with a stopper without inclusion of bubbles.

Subsequently the solution is mixed gently. After sedimentation of flocculants (about 1 hour) the clear supernatant is removed and the sediment is added with:

- 0.2 ml of a 3 % NaN3 solution

- 0.5 ml of a 50 % KJ solution

- 2.0 ml phosphoric acid (density orthophosphoric acid 85 % at 20 °C: δ = 1.71)

After 5 minutes the sediment has resolved again and the produced iodine is titrated with 0.01 N Na2S2O3. At the end of the titration curve starch-solution is added as an indicator solution and the titration is continued until the solution has cleared its colour. All other samples are handled equivalently.

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

BOD5= 0 mg/g O2

Executive summary:

The substance has been tested for screening test of BOD5 according to the internal method Ciba Geigy n° A-15. The substance shows a BOD5= 0 mg/g O2

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD screening test

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

August 5, 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Ciba Geigy internal method A-16

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: DEV H4 method

Deviations:

not specified

Principles of method if other than guideline:

Within this procedure chemical oxygen demand (COD) is defined as the equivalent amount of oxygen in terms of amount of potassium dichromate that is consumed by reacting with the oxidizable components of 1 g of sample material dissolved in 1 liter water under the given reaction conditions.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 22 mg/L

Based on:

other: TOC

Parameter followed for biodegradation estimation:

O2 consumption

Key result

Remarks on result:

not measured/tested

Parameter:

COD

Value:

ca. 675 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 0 mg O2/g test mat.

Calculation of the chemical oxygen demand:

[(a-b) x N x 8.0 x 1000]/v= mg/g O2

a = oxygen consumption [mL] 0.12 n (NH4)2Fe(SO4)2 x 6 H2O for blank sample(s)

b = oxygen consumption [mL] 0.12 n (NH4)2Fe(SO4)2 x 6 H2O for actual sample(s)

N = normality of the (NH4)2Fe(SO4)2 x 6 H2O solution

V = sample volume in [mL] calculated based on the number of dilution steps

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

COD= 675 mg/g O2

Executive summary:

The substance has been tested for screening test of COD according to the internal method Ciba Geigy AG n° A-16 and DEV H4. The substance shows a COD= 675 mg/g O2

Description of key information

The substance is not ready biodegradable and non readily biodegradable     
    
    
    
    
    
    
    
    
    
    

It was confirmed that the substance is not inherent biodegradable and is not ready biodegradable.