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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72-h ErL50 and ErL10 values are both >1000 mg/L (loading rate), respectively in freshwater algae (P. subcapitata).

Key value for chemical safety assessment

Additional information

The toxicity to aquatic algae was determined in a test according to OECD guideline No 201 and in compliance with GLP criteria (Institute of Industrial Organic Chemistry Branch Pszczyna, 2015).

In this study freshwater algae (P. subcapitata) were exposed to test substance loading rates of 0 (control), 12, 37, 111, 333 and 1000 mg/L for 72 hours under static conditions. The filtrate of all loading rates were analytically verified based on total organic carbon (TOC) content at exposure initiation, after 24 and 48 hours expsoure (in the highest test item concentration and the control) and at exposure termination. This analytical approach did not account for any degradation or metabolisation effects which may have occurred during exposure. The analytical concentrations for the filtrate were below the LoQ for TOC of of 1.0 mg C/L in all test item concentrations and the control. Morphology observations of the algae were performed at exposure termination. The average specific growth rates and yield were calculated based on the numbers of cells recalculated from absorbance values measured at 24, 48 and 72 h for the cell number based on the standard curve. Absorbance values were recalculated for algae cell density, the average section-by-section specific growth rates and yield calculated for the whole exposure.

Based on observations of these parameters, the 72 -h EL50 and 72 -h EL10 values for growth rate were both determined at >1000 mg/L. The 72 -h EL50 and 72 -h EL10 values for yield were determined at >1000 mg/L and 224.9 mg/L, respectively. All effect values are determined as loading rates of the nominal test item concentrations.