Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides

Endpoint summary

Additional information

Biodegradation in water: screening tests

The predicted study for the test compoundFormaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides(CAS No. 83968-28-9) and its read across substance were reviewed for the biodegradation end point which is summarized as below:

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compoundFormaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides(CAS No. 83968-28-9). The screening test inherent to the biodegradability of the substance was calculated using the software BIOWIN v4.10. The results indicate thatFormaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorideis not expected to be readily biodegradable.

5-days Closed bottle test to determine biological oxygen demand and thereby % degradation during the test period was conducted for the read across substanceN, N- diethylaniline(CAS No.91-66-7)[UERLStudy Report, Sustainability Support Services (Europe) AB (Report no. UERL/BOD-005/91 -66 -7/2014),2014].The test system included control, test substance and reference substance. The concentration of test and reference substance (sodium acetate) chosen for the study were 2 mg/l & 5 mg/l respectively while that of inoculum (diluted soil suspension) was 0.5 ml/l. Further, ThOD (Theoretical oxygen demand) of test substance was determined by calculation/experimentally. % Degradation was calculated using the values of BOD and ThOD for test substance and reference substance.The BOD₅value of N,N- diethylaniline(CAS No.91-66-7) was observed to be 0.375 mgO₂/mg. ThOD was determined by calculation as 2.78 mgO₂/mg. Accordingly, the % degradation of the test substance after 5 days of incubation at 20°C according to closed bottle test was found to be 13.48%. Based on the results, the test item, under the test conditions, was found to be inherently biodegradable at 20°C over a period of 5 days.

 

 

In 2 weeks study (J-CHECK database, 1977) of biodegradation of N,N-diethylaniline, Concentration of inoculum i.e, sludge is 30 mg/l and initial test substance conc. used in the study is 100 mg/l. The percentage degradation of test substance was found to be 0% by BOD, and 1% by HPLC. Thus it is concluded that substance N,N-diethylaniline is found to be not readily biodegradable under test conditions.

Biodegradation screening test (J-CHECK, 2016) was conducted for 14 days (2 weeks) for evaluating the percentage biodegradability of the test substance. Concentration of inoculum i.e, sludge is 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 1.9% by BOD, 12.3% by TOC and 0% by GC. Thus, the substance N,N-Dimethylaniline was considered to be not readily biodegradable in water.

On the basis of theoverall reported resultsoftarget and its read across substance,it can be concluded thatthe substancewas found to be not-readily biodegradable in water.

Biodegradation in water and sediment

Half life period of Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides in water is observed to be 37.5 days (900 hrs.) while in sediment it is 337.5 days (8100 hrs) (EPI Suite, 2016). Based on these half life values of Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides, it is concluded that the chemical is not persistent in water and persistent in sediment.

Biodegradation in soil

Based on EPI prediction Level III Fugacity Model (Full-Output), the estimated half life of biodegradation of Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides in soil was obtained to be 75 days. Based on this half life value of

Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides, it is concluded that the chemical is not persistent in the soil environment.