4,11-diamino-2-[3-(2-methoxyethoxy)propyl]-1H-naphth[2,3-f]isoindole-1,3,5,10(2H)-tetrone

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

not specified

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

The test organism used for the study was Scenedesmus subspicatus CHODAT, Strain No. 8681 SAG, supplied by the Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universitat, D-3400 Göttingen, F.R.G. The algae were grown in the laboratories of RCC under standardized conditions according to the OECD Guideline No. 201.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

Not available

Test temperature:

21-23 °C

pH:

Not available

Dissolved oxygen:

Not available

Salinity:

Not available

Nominal and measured concentrations:

The nominal concentrations tested were 0.25, 0.50, 1.0, 2.0 and 4.0 mg test substance/L and a control without any additions and a solvent control group.

Details on test conditions:

Not available

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

96 h

Dose descriptor:

EC0

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

96 h

Dose descriptor:

EC100

Effect conc.:

> 4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

EC0

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

None

Results with reference substance (positive control):

Not available

Reported statistics and error estimates:

None

None

Validity criteria fulfilled:

yes

Conclusions:

EµC50 (0 - 96 h) - > 4.0 mg test substance/l

Executive summary:

The present study was performed to determine the influence of FAT 36152/D on the growth of the green alga Scenedesmus subspicatus Chodat in a 96-hour static test according to the OECD Guideline No. 201. The nominal concentrations tested were 0.25, 0.50, 1.0, 2.0 and 4.0 mg/l and a control without any additions and a solvent control group. FAT 36152/D has obviously an inhibition effect on the growth of Scenedesmus subspicatus. The inhibition was calculated for both parameters, the algae biomass (determined as the area under the growth curve) and the growth rate µ:

 

Biomass:

- EbC50 (0 - 96 h) = 2.0 mg test substance/l

- EbC0 (0 - 96 h) <0.25 mg test substance/l

- EbC100 (0 - 96 h) >4.0 mg test substance/l

 

Growth rate µ:

EµC50 (0 - 96 h) >4.0 mg test substance/l

EµC0 (0 - 96 h) <0.25 mg test substance/l

 

The measured test substance concentrations in the analysed test media of the nominal concentrations 1.0 and 4.0 mg/l amounted from below the lowest calibration point of the analytical method to 23.0 % of the nominal values. Due to the fact that the test substance is not soluble in water, homogeneous samples for the analytical measurements could obviously not be taken. Samples with a nominal concentration lower than 1.0 mg/l were not detectable. Since the measured concentration in the stock solution amounted to 93.0 % of the nominal value, the reported results are related to the nominal concentrations of the test substance.

Description of key information

The EµC50 (0 - 96 h)  for FAT 36152/D was considered to be >4.0 mg test substance/l.

Additional information

The influence of FAT 36152/D on the growth of the green alga Scenedesmus subspicatus Chodat was investigated in a 96-hour static test according to the OECD Guideline No. 201.The nominal concentrations tested were 0.25,0.50,1.0, 2.0 and 4.0 mg/l and a control without any additions and a solvent control group. FAT 36152/D has obviously an inhibition effect on the growth of Scenedesmus subspicatus. Based on the findings of the study, EµC50 (0 - 96 h) was considered to be >4.0 mg test substance/l.