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EC number: 920-338-0 | CAS number: 109236-76-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline- and GLP-Study with minor or no deficiencies.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- docosyl 4-hydroxybenzoate
- EC Number:
- 920-338-0
- Cas Number:
- 109236-76-2
- Molecular formula:
- C29 H50 O3
- IUPAC Name:
- docosyl 4-hydroxybenzoate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The stock solution (100 mg/L test item were weighed out) was prepared with dilution water one day prior to application.
The stock solution was shaken with 20 rpm for 24 hours (rotating shaker 3040, GFL). Undissolved particles were removed by membran filtration (0.45 pm, RC, Machery-Nagel).
Limit concentration: Saturated solution*
The tested saturated solution prepared with 100 mg test item/L was visually clear throughout exposure. Due to the low solubility of the test item the measured concentrations of 4-Hydroxybenzoic acid behenylester in the fresh (0 hours) and old media (48 hours) of the saturated* solution were
below the limit of quantification (LOQ = 4 µg/L).
*Saturated solution: The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium, acc. to OECD Series, No. 23 (2000)6.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism: Daphnia magna STRAUS (Clone 5)
Reason for the selection of the test organism: Daphnia magna belongs, in accordance with the study guideline, to the suitable Daphnia types and is
bred at the test facility.
Origin: Institut fur Wasser-, Boden- und Lufthygiene (WaBoLu)
Breeder: DR.U.NOACK-LABORATORIEN, Käthe-Paulus-Str. 1, D-31157 Sarstedt, Germany
Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µE-m^-2s^-1.
Culture medium: Elendt M4, according to Elendt (1990), modified to a total hardness of 160 to 180 mg CaC03/L.
Feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata, Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106ceils/mL
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Details on test conditions:
- Test duration: 48 hours
Test vessels : Glass beakers (4 cm ID x 7 cm H), 50 mL capacity
Test volume: 20 mL
Dilution water: According to Directive 92/69/EEC Method C.2, Annex 1
Component Concentration [mg/L]
KCL 5.76
NaHC03 64.8
CaCl2 x 2 H20 294
MgS04x7 H20 123
pH-value: 7.8 ± 0.2
Number of study organisms: 20 animals, divided into 4 parallel samples, each with 5 animals per limit concentration and control
Age of test organisms: 2 to 24 hours old daphnids were used for the definitive study. They were obtained by removing the mother
animals twice within 22 hours.
Acclimatization: At least 2 h in dilution water
Test temperature: 18-22 °C, ± 1 °C constant
Illumination Photoperiod: Diffuse light, illumination strength max. 20 uE-m"2 - s"1, 16/8 h light/dark cycle
Feeding: The daphnids were not fed during the study.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 4 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
Any other information on results incl. tables
The acute immobilization of the test item 4 -Hydroxybenzoic acid behenylester to Daphnia magna (STRAUS) was determined according to OECD 202 (2004). The study was conducted as a limit test with the saturated solution of the test item as limit concentration under static conditions over a period of 48 h. 20 test organisms were exposed to the limit concentration and control.
The saturated solution and the control were analysed via LC-MS/MS after 0 hours (new media) and 48 hours (ofd media).
Due to the low solubility of the test item the measured concentrations of 4-Hydroxybenzoic acid behenylester in the fresh (0 hours) and old media (48 hours) of the saturated solution were below the limit of quantification (LOQ = 4 µg/L). All effect values are given based on the saturated solution prepared with 100 mg 4 -Hydroxybenzoic acid behenylester/L. The water quality parameters (pH-value and dissolved oxygen concentration), measured at 0 and 48 hours, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution prepared with 100 mg 4-Hydroxybenzoic acid behenylester/L. The effective concentration of the substance in the solution was below the LOQ of 4ppb (4 µg/l). No effect on Daphnia magna was observed at this concentration. Therefore the substance is not classified.
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