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EC number: 207-787-1 | CAS number: 494-19-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- April 28, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented and reported study, conducted equivalent to internationally accepted technical guideline in recognized research organization.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- , 1984
- Deviations:
- yes
- Remarks:
- : instead of a centrifuged sludge a settled sludge was used. Due to the poor solubility of the test substance at test concnetrations, no stock solution was prepared. The test substance was given directly into the medium. Therefore, a constant factor betw
- GLP compliance:
- yes
Test material
- Reference substance name:
- 10,11-dihydro-5H-dibenz[b,f]azepine
- EC Number:
- 207-787-1
- EC Name:
- 10,11-dihydro-5H-dibenz[b,f]azepine
- Cas Number:
- 494-19-9
- Molecular formula:
- C14H13N
- IUPAC Name:
- 10,11-dihydro-5H-dibenzo[b,f]azepine
- Details on test material:
- Identification Code (as cited in study report) : PBS 2354.0
Generic/Trade name : Iminodibenzyl dest.
Batch No.: P. 4/92
Appearance: yellow solid
Purity : 97.1 %
Solubility (in water): < 2 mg/l (20°C)
Received : 08/04/93
Storage : room temperature
Stability : 12/93
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 20.9, 8.4, 4.0, 1.3, 0.6 and 0.4 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with dechlorinated drinking water.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 27/04/93. The pH after collection was 7.2.
- Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 8.0.
- Initial biomass concentration: no data
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 ± 2 °C
- pH:
- 8.4 - 8.6
- Dissolved oxygen:
- no data
- Salinity:
- not applicable (freshwater)
- Nominal and measured concentrations:
- nominal 104.5, 42.0, 20.0, 6.5, 3.0, 2.0 and 0 (blank) mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml BOD flasks with gas inlet
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1.57 g/l (dry weight
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking-water
- Intervals of water quality measurement: The pH was measured after 3h aeration.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration inhibition after 3-hour aeration
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 - 3.1. Due to the poor solubility of the test substance at test concentrations, no stock solution was prepared. The test substance was given directly into the medium. Therefore, a constant factor between the test concentrations could not been achieved. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol; 32.0, 10.0 and 3.2 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Oxygen consumption rates were 47.8 and 49.6 mg/l/h for the controls and 46.1-49.6 mg/l/h for the test substance.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3h) = 18.4 mg/l (accepted range: 5-30 mg/L) - Reported statistics and error estimates:
- The Results ( ECxx Values ) were calculated with linear regression.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Criteria of OECD Guideline 209: The difference between the controls was within 15% of each other. The EC50 of 3,5-dichlorophenol was in the accepted range of 5-30 mg/l.
- Executive summary:
In a 3-h toxicity study activated sludge from a sewage treatment plant was exposed to PBS 2354.0 in accordance with OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test). The study was performed in compliance with GLP. Reliability grade 1 was assigned. Tested concentrations were 0 (controls), 2.0, 3.0, 6.5, 20.0, 42.0, and 104.5 mg/l. PBS 2354.0 was not toxic to waste water (activated sludge) bacteria up to and including a nominal concentration of 104.5 mg/l. Thus, the 3h-EC20, -EC50 and -EC90 was above 100 mg/L.
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