Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-240-8 | CAS number: 17199-29-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 26-05-2002 to 27-05-2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- L-2-hydroxy-2-phenylacetic acid
- EC Number:
- 210-276-6
- EC Name:
- L-2-hydroxy-2-phenylacetic acid
- Cas Number:
- 611-71-2
- Molecular formula:
- C8H8O3
- IUPAC Name:
- hydroxy(phenyl)acetic acid
- Details on test material:
- ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %; impurities: 0.1 % mandelic acid amide)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - preparation of inoculum: washed, conc. of dry matter: 5 g/l, aerated during the night, 50 ml were added to a total volume of 250 ml
- initial concentration during test: 1 g/L dry weight
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +- 2 °C
- pH:
- see table 1
- Dissolved oxygen:
- - oxygen concentration during aeration: > 2.5 mg/l
- oxygen concentration immediatley before measurement: > 6.5 mg/l
- duration of measurement of oxygen consumption: 8-10 min - Nominal and measured concentrations:
- nominal: 1000 mg/L
- Details on test conditions:
- - test vessel: Erlenmeyer flask (250 ml)
- test volume: 250 ml
- test assays: blank control (3x), reference substance (3x) and test substance (1000 mg/l)
- synthetic medium: 8 ml (100-fold concentrated OECD medium)
- inoculum: 50 ml (final conc. of dry matter: 1 g/l)
- dilution water: deionised water - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Results with reference substance (positive control):
- - reference substance: 3,5-dichlorophenol
effect values for reference substance (30 min, mg/l):
EC20 ca. 3.3
EC50 ca. 15
EC80 ca. 85
Any other information on results incl. tables
Table 1: Oxygen consumption and pH values
Parameter | BC average | TS | RS1 | RS2 | RS3 | |||||
| start | 6 min | start | 6 min | start | 6 min | start | 6 min | start | 6 min |
Oxygen concentration (mg/L) | 6.8-7.8 | 5.0-6.5 | 6.9 | 5.0 | 6.9 | 5.0 | 8.1 | 7.1 | 8.6 | 8.3 |
oxygen consumption rate (mg O2/L/h) | 18 | 19 | 19 | 10 | 3 | |||||
specific oxygen consumption rate | 18 | 19 | 19 | 10 | 3 | |||||
change in oxygen consumption rate compared to BC (%) | - | -6 | -6 | 44 | 83 | |||||
pH | 7.1 | 7.6 | 7.2 | 7.3 | 7.0 | |||||
pH after test end (30 min) | - | 7.8 | - | - | - |
BC = blank control
RS = reference substance (reference substance conc.: RS1: 1 mg/l; RS2: 10 mg/l; RS3: 100 mg/l)
TS = test substance
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
- Executive summary:
An experimental study according to OECD guideline 209 and GLP regulations was conducted to assess the toxicity to microorganism. An inoculum of domestic activated sludge with an initial concentration of 1 g/L dry weight was exposed to 1000 mg/L test item for 30 min in a static system. In addition, a reference substance (3,5-dichlorophenol) and a blank control was tested. The respiration inhibition was determined based on the oxygen consumption. After 30 min no inhibition of the oxygen consumption was observed. The effect values were determined to be > 1000 mg/L. Therefore, an inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.