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EC number: 419-640-0 | CAS number: 68784-14-5 ALLYL SUCROSE
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) was investigated using a method compatible with OECD 203 and EU Method C.1. The 96-Hour LL50 was found to be greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF.
Key value for chemical safety assessment
Additional information
Introduction
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.
Methods
Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a water accommodated fraction (WAF).
In accordance with the recommendations of REACh, the test was conducted according to the threshold approach recommended by ECHA. Using this approach the lowest EL50 value from either the Algal Growth Inhibition Study or Acute Toxicity to Daphnia magna study is set as the threshold loading rate and a "Limit test" is conducted at this threshold loading rate. If no mortalities are observed this indicates that fish are notthe most sensitive species and that the LL50 is greater than the threshold loading rate. Therefore as the EL50 value obtained for each of the studies indicated were greater than 100 mg/L loading rate WAF, the test was conducted at a single loading rate of 100 mg/L loading rate WAF to ensure toxicity was not observed atthis loading rate.
Seven fish were exposed to a WAF of the test item, at a single nominal concentration of 100 mg/L loading rate WAF for a period of 96 hours at a temperature of approximately 15 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Results
Chemical analysis of the 100 mg/L loading rate WAF test preparations at 0 and 72 hours (fresh) showed measured concentrations of 30 and 21 mg/L were obtained respectively and at 24 and 96 hours (aged) showed measured concentrations of 32 and 20 mg/L respectively. Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
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