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EC number: 451-060-3 | CAS number: 122886-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 April 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the OECD 209 Guideline and following GLP principles. All validity criteria of the Guideline 209 (1984) were accepted.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- The test solutions at 31.6, 100, 316 and 1000 mg/L were prepared by direct addition of the test item in the test vessels and vigorous agitation of these solutions for a few minutes before the beginning of the test (i.e. just before addition of the inoculum).
The test solution at 10 mg/L was prepared by means of a suspension at 20 mg/L in dechlorinated water because the test item quantity necessary for direct addition to the test flask was too low to be accurately weighed(< 10 mg). The suspension was agitated for 1 hour and 40 minutes in order to solubilize as much of the test item as possible and obtain an homogeneous suspension. The test solution was prepared immediately by further dilution of this suspension, maintained under agitation, with dechlorinated water.
A reference stock solution was prepared by dissolving 0.5 g of the reference item, 3,5-dichlorophenol, in 10 mL of NaOH at 1 N. The obtained preparation was then diluted to 30 mL with purified water. Aliquots of H2S04 at 1 N were added while stirring until the point of incipient precipitation was obtained (approximately 8 mL of H2S04 at 1 N were required). The solution was made up to 1000 mL using purified water. The reference stock solution was agitated for 18 hours. Reference solutions were prepared by further dilution of the stock solution with dechlorinated water. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Origin: the water treatment plant of Evreux (France)
- Preparation of inoculum for exposure: the inoculum was left to settle and the supernatant rejected. It was then filtered (to remove the biggest particles) and washed with dechlorinated water. Four samples of 50 mL were taken to determine the content in suspended matter (dry weight). The concentration of sludge (2.6 g/L) was then adjusted by allowing it to form floccules in the stock flask (by stopping aeration) and then rapidly decanting off a portion of the supernatant until a predetermined volume was reached. The quantity of suspended matter was 4g/L± 10%.
- Pretreatment: The inoculum was maintained under agitation for 1 day before the test. As the sludge was not used immediately, 50 mL of sewage feed were added, per liter, just before agitation. Synthetic sewage feed was prepared using dechlorinated water and analytical grade reagents following OECD recommendations.
- Initial biomass concentration: 1.6 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 +/- 20 mg/L as CaCO3
- Test temperature:
- 21 -22 ºC
- pH:
- The pH of the test solution at 1000 mg/L was 7.7.
The pH of the stock solution at 20 mg/L was 7.9 after agitation.
The pH of the reference stock solution was 7.7 after agitation. - Dissolved oxygen:
- Dissolved oxygen concentrations are not reported, only respiratory uptake in mg O2/L h.
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations of 10, 31.6, 100, 316 and 1000 mg/L
All concentrations of KY-UN in this test were higher than the water solubility of this item and should therefore be considered as loading rates (solutions including the dissolved and undissolved fractions of the item). - Details on test conditions:
- TEST SYSTEM
- Test vessel: opaque BOD flask
- Aeration: air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurements
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1.6 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechloriniated water
OTHER TEST CONDITIONS
- Adjustment of pH: The pH of inoculum was adjusted, if necessary, to 6.0- 8.0 using NaHC03 or HCI.
- Photoperiod: not stated
- Light intensity: no special precautions were taken
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Measurement of oxygen concentration of the above solutions was determined using an oxygen probe placed into an opaque BOD (Biological Oxygen Demand) flask filled with the test or reference solution which was agitated with a magnetic stirrer. The oxygen probe was connected to a meter equipped with a chart recorder.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- See attached Table 1.
- Results with reference substance (positive control):
- The 3h-EC50 was determined to be 11 mg/L (95% CI 9.24-13 mg/L)
- Validity criteria fulfilled:
- yes
- Remarks:
- see "Overall remarks'.
- Conclusions:
- The substance is not toxic to STP microorganisms up to 1000 mg/L.
- Executive summary:
The effect of the test substance on the respiration rate of microorganisms was assessed according to the OECD 209 and following GLP principles. Under the experimental conditions, the 3-hour EC50 of the test item KY -UN is > 1000 mg/L (solution including the dissolved and undissolved fractions of the item) for activated sludge. The test item should be considered as non-toxic for the micro-organisms of a water treatment plant.
Reference
Description of key information
Based on the results of an activated sludge respiration inhibition study, the substance is not toxic to STP microorganisms up to 1000 mg/L.
Key value for chemical safety assessment
Additional information
The effect of the test substance on the respiration rate of microorganisms was assessed according to the OECD 209 guideline and following GLP principles. Under the experimental conditions, the 3-hour EC50 of the test item KY -UN is > 1000 mg/L (solution including the dissolved and undissolved fractions of the item) for activated sludge. The test item should be considered as non-toxic for the micro-organisms of a water treatment plant (as at 1000 mg/L only 9% inhibition was observed).
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