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EC number: 215-236-1 | CAS number: 1314-56-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-01-07
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Principles of method if other than guideline:
- In contrast to the guideline, it was decided to use copper (II) suphate pentahydrate instead of the prescribed 3,5-dichlorophenol. The usage of dichlorophenol is unsuitable for routine lab use.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diphosphorus pentaoxide
- EC Number:
- 215-236-1
- EC Name:
- Diphosphorus pentaoxide
- Cas Number:
- 1314-56-3
- Molecular formula:
- O10P4
- IUPAC Name:
- tricyclo[3.3.1.1³,⁷]tetraphosphoxane-1,3,5,7-tetrone
- Details on test material:
- - Name of test material (as cited in study report): Phosphorpentoxid
- Molecular formula: P4O10
- Molecular weight: 283.9 g/mol
- Physical state: Solid, white powder
- Analytical purity: 99.7 % (m/m)
- Purity test date: 2009-09-23
- Lot/batch No.: DEH2 018965
- Expiration date of the lot/batch: 2011-09-08
- Storage condition of test material: Stored in a desiccator at room temperature, protected from light.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Agitation
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading:100 - 180 - 320 - 580 - 1000 mg/L without adjustment of pH
+ 320 - 580 - 1000 mg/L adjusted to pH 7
- Controls: 2 control samples without test item were included, one at the beginning and one at the end of the test.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at SARSTEDT (Municipal sewage treatment plant of 31157 SARSTEDT, GERMANY)
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 4.0 g/L +/- 10 %
- Dry sludge concentration: 4.12 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 19.9 °C
- pH:
- 6.74
- Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Composition of test medium: 200 mL inoculum + 16 mL synthetic waste water (acc. to the guideline) + 284 mL autoclaved tap water + test substance concentration (directly weighing out in each test vessel)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Determination of respiration rates subsequently to an incubation period of 3 h
TEST CONCENTRATIONS
- Range finding study
Inhibitions in the Preliminary Tests
Nominal Test Item
Concentration [mg/L] Inhibition
[%]
10 (pH: 7.27) -5
100 (pH: 5.35 7.00) -2
1000 (pH: 2.16 7.08) 42
10 -2
100 5
1000 98 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 other: mg/L (with adjustment of pH); 215 (209-220) mg/L without adjustment
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 101 mg/L - Reported statistics and error estimates:
- The EC-values of the test item (without adjustment of pH) were calculated by sigmoidal dose-response regression using software GraphPad Prism4, GRAPHPAD SOFTWARE, INC. Calculation of the confidence intervals for the EC50-value was carried out using standard procedures.
The EC-values of the test item (with adjustment to pH 7) and the EC50-value of the reference item were calculated by probit analysis using software SIGMAPLOT (Windows), SPSS CORPORATION. Probit values were taken from WEBER (1986).
Any other information on results incl. tables
From the oxygen depletion the respiration rates were calculated in mgO2/(L×h).
The inhibition of the respiration rates from the test and reference item concentrations in % was calculated in comparison with the respiration rates from the control.
Respiration Rates and Inhibition of Respiration of Test and Reference Item Concentrations
Concentration |
Respiration rate |
Inhibition |
|||||
[mg/L] |
[mgO2/(L×h)] |
[%] |
|||||
Test item |
1000 |
0.0 |
100 |
||||
580 |
0.0 |
100 |
|||||
320 |
2.8 |
91 |
|||||
180 |
21.2 |
31 |
|||||
100 |
26.0 |
15 |
|||||
1000 # |
17.2 |
44 |
|||||
580 # |
24.4 |
20 |
|||||
320 # |
27.6 |
10 |
|||||
Control 1 |
– |
29.2 |
– |
||||
Control 2 |
– |
32.0 |
– |
||||
Reference item |
180 |
8.4 |
73 |
||||
100 |
15.2 |
50 |
|||||
58 |
22.4 |
27 |
The EC20-, EC50- and EC80-values were calculated by sigmoidal dose-response regression for the test item concentrations without adjustment of pH and by probit analysis for the test item concentrations adjusted to pH 7.
Table 6: EC-Values with Confidence Interval of Phosphorpentoxid
Values without adjustment of pH |
Values with adjustment of pH |
|||
EC-values |
Confidence interval |
EC-values |
Confidence interval |
|
[mg/L] |
P = 95 [%] [mg/L] |
[mg/L] |
P = 95 [%] [mg/L] |
|
EC20 |
147 |
- |
524 |
- |
EC50 |
215 |
209 - 220 |
>1000 |
>1000 |
EC80 |
275 |
- |
>1000 |
- |
Raw Data and Respiration Rates of Test Item, Control and Reference Item
Concentration |
Oxygen-concentration* |
Measured distance** |
Respiration rate |
|
[mg/L] |
[mgO2/L] |
[mm/3 min] |
[mgO2/(L×h)] |
|
Test item |
1000 |
8.68 |
0 |
0.0 |
580 |
8.73 |
0 |
0.0 |
|
320 |
8.47 |
7 |
2.8 |
|
180 |
7.29 |
53 |
21.2 |
|
100 |
6.57 |
65 |
26.0 |
|
1000 # |
7.12 |
43 |
17.2 |
|
580 # |
7.54 |
61 |
24.4 |
|
320 # |
6.32 |
69 |
27.6 |
|
Control 1 |
– |
7.03 |
73 |
29.2 |
Control 2 |
– |
7.52 |
80 |
32.0 |
Reference item |
180 |
8.13 |
21 |
8.4 |
100 |
7.85 |
38 |
15.2 |
|
58 |
7.58 |
56 |
22.4 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
A Respiration Inhibition Test according to OECD Guideline No. 209 was performed with the test item Phosphorpentoxid (batch-no. DEH2 018965) on 2010-01-07 at Dr.U.Noack-Laboratorien, D-31157 Sarstedt. Test system was activated sludge of the municipal treatment plant of D-31157 Sarstedt. The test was performed under static conditions with the nominal concentrations 100 - 180 - 320 - 580 - 1000 mg/L without adjustment of pH and 320 - 580 - 1000 mg/L adjusted to pH 7 to differentiate between pH effects and test item effects. The respiration rates of control, reference and test item concentrations were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The inhibition of the test item ranged from 15 % to 100 %. The neutralised test item concentrations showed inhibitions from 10 % to 44 %. The EC20-, EC50- and EC80-values were calculated by sigmoidal dose-response regression for the test item concentrations without adjustment of pH and by probit analysis for the test item concentrations adjusted to pH 7.
Table 1: EC-Values with Confidence Interval of Phosphorpentoxid
Values without adjustment of pH
Values with adjustment of pH
EC-values
Confidence interval
EC-values
Confidence interval
[mg/L]
P = 95 [%]
[mg/L]
[mg/L]
P = 95 [%]
[mg/L]
EC20
147
-
524
-
EC50
215
209 - 220
>1000
>1000
EC80
275
-
>1000
-
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 101 mg/L.
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