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EC number: 202-411-2 | CAS number: 95-33-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
- NOAEL = 80 mg/kg bw/day – equiv. OECD 422 – gavage – MHWJ (1997)
- NOAEL = 250 mg/kg bw/day – nonTG 28-day subacute – dietary – Monsanto (1980)
Key value for chemical safety assessment
- Toxic effect type:
- dose-dependent
Repeated dose toxicity: via oral route - systemic effects
Link to relevant study records
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 407 (Repeated Dose 28-Day Oral Toxicity Study in Rodents)
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for 28-day repeated dose toxicity testing of chemicals (Japan)
- Deviations:
- no
- Principles of method if other than guideline:
- N-cyclohexyl-2-benzothiazolesulfenamide was studied for oral toxicity in rats in a 28-day repeat dose toxicity test at doses of 0, 25, 80, 250 or 800 mg/kg bw/day.
- GLP compliance:
- yes
- Limit test:
- no
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Duration of treatment / exposure:
- 28 days
- Frequency of treatment:
- daily
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Remarks:
- Vehicle control
- Dose / conc.:
- 25 mg/kg bw/day (nominal)
- Dose / conc.:
- 80 mg/kg bw/day (nominal)
- Dose / conc.:
- 250 mg/kg bw/day (nominal)
- Dose / conc.:
- 800 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- Six per dose and sex.
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Exposure period: 28 days
Post-exposure period: with and without 14 days recovery period - Observations and examinations performed and frequency:
- Signs of general condition,food consumption, body weight gain, Hematoloty, urinalysis
- Sacrifice and pathology:
- Organs weights, histopathological examination.
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Signs of loss of general condition like piloerection and soiled fur were observed in females of the 800 mg/kg bw group.
- Mortality:
- no mortality observed
- Description (incidence):
- All animals survived until the end of the study.
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- Suppression of food consumption and body weight gain was noted in females of the 250 mg/kg group and males and females of the 800 mg/kg bw group.
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Description (incidence and severity):
- Suppression of food consumption and body weight gain was noted in females of the 250 mg/kg group and males and females of the 800 mg/kg bw group.
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not examined
- Haematological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Shortening of prothrombin time in males of the 250 and 800 mg/kg bw groups.
Decreased hematocrit, reticulocyte count and platelet count in females of the 800 mg/kg bw group. - Clinical biochemistry findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Total protein content was decreased in males of the 250 and 800 mg/kg bw groups.
Chloride levels were reduced in males and females of the 800 mg/kg-group. Calcium level was elevated and sodiumlevel reduced in females of the 800 mg/kg bw group. - Urinalysis findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Increase of ketone bodies in males of 250 and 800 mg/kg bw groups.
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Distended cecum in females of the 800 mg/kg bw group. Increased relative kidney weights in males and females of the 800 mg/kg bw group.
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Increased deposition of hyaline droplets in the renal proximal tubular epithelium in males of the 250 and 800 mg/kg w groups. After the 14 days recovery the histopathological changes in the kidneys tended to recover and the other substance-related changes had disappeared.
- Histopathological findings: neoplastic:
- not examined
- Key result
- Dose descriptor:
- NOAEL
- Effect level:
- 80 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: based on signs of coagulopathy and kidney adversity in male rats, suppression of food consumption and body weight gain in female rats
- Key result
- Dose descriptor:
- LOAEL
- Effect level:
- 250 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- haematology
- histopathology: non-neoplastic
- Key result
- Dose descriptor:
- LOAEL
- Effect level:
- 250 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- body weight and weight gain
- Conclusions:
- No relevant systemic toxic effects were observed in male and female rats after repeated oral administration of 80 mg/kg bw and day CBS. A NOAEL of 80 mg/kg bw/day was assigned for both sexes based on signs of a coagulopathy and adverse effects in the kidney of male rats, suppression of food consumption and body weight gain in female rats (MHWJ 1997).
- Executive summary:
Study design
The test substance CBS was evaluated in a subacute gavage study with Crj: CD (SD) rats, which was performed mostly in accordance to regulation requirements (mostly according to OECD TG 407) (MHWJ 1997). Male and female rats (6 per dose and sex) were administered with 0, 25, 80, 250 and 800 mg/kg bw/day for 28 days and were scarified on day 29. Another control and 800 mg/kg bw/day group were treated for 28 days and then kept for a recovery period of 14 days before sacrifice on day 43. In this study data on haematology, clinical biochemistry, organ weights and incidences of histopathological findings were provided.
Results
No mortality occurred during the study period. After administration of 800 mg/kg bw/day signs associated with loss of general condition like piloerection and solid fur were observed in female rats. Suppression of food consumption and body weight gain was observed in females given ≥250 mg/kg bw/day (significant p=0.01 at 800 mg/kg bw/day) and in males at 800 mg/kg bw/day.
Hematology revealed statistically significant shortening of the prothrombin time in males given = 250 mg/kg bw/day, and statistically significant decrease in hematocrit value, reticulocyte count and platelet count for the 800 mg/kg bw/day females.
Clinical biochemistry revealed statistically significant decreases in alanine aminotransferase (ALAT) for males of all CBS treated groups and for females given ≥ 80 mg/kg bw/day, in total protein for males treated at ≥ 250 mg/kg bw and day, in chloride levels in males and females at 800 mg/kg bw and day and in sodium levels in females at 800 mg/kg bw/day. In addition, calcium concentration was elevated in females at the 800 mg/kg bw/day dose group.
Urinalysis revealed an increase of ketone bodies in males ≥ 250 mg/kg bw/day.
The statistically significant increase in relative kidney weights in the 800 mg/kg bw/day in males was considered to be associated with an increase in deposition of hyaline droplets in the proximal tubular epithelium of the kidneys in male rats evident at 250 and 800 mg/kg bw/day. This effect in male kidney showed a clear tendency towards reversibility. All other changes were completely reversible after the 14 day recovery period.
Conclusion
In summary, CBS-related effects were present in male and females Crj: CD (SD) rats at = 250 mg/kg bw/day. There were signs of coagulopathy of the blood in both sexes and effects in the kidney of male rats. No relevant systemic toxic effects were observed in male and female rats after repeated oral administration of 80 mg/kg bw and day CBS. Based on these findings, the author considered a NOAEL of 80 mg/kg bw/day appropriate for both sexes (MHWJ 1997).
Reference
All animals survived until the end of the study.
Males:
25, 80 mg/kg bw: no adverse effects (decrease of GPT* at 25, and 80 mg/kg bw)
250 mg/kg bw: increase of ketone bodies, shortening of the prothrombin time, on blood chemical examination: total protein was decreased,
Histopathology: an increase in deposition of hyaline droplets in the renal proximal tubular epithelium.
800 mg/kg bw: Suppression of food consumption and body weight gain, urin analysis revealted an increase of ketone bodies,shortening of the prothrombin time,on blood chemical examination: total protein was decreased, reduced chloride levels,
Histopathology: an increase in deposition of hyaline droplets in the renal proximal tubular epithelium, increase of the relative kidney weights
No abnormalities were detected in: pancreas, stomach, small intestine, large intestine, thyroid, parathyroid, spleen, lymph node, bone marrow or brain, 1/6 males of the 800 mg/kg bw group + 14 d recovery showed a moderate effect on the testis (atrophy, seminiferous tubule, diffuse).
Females
25,80 mg/kg bw: no adverse effects (decrease of GPT* at 80 mg/kg bw)
250 mg/kg bw: Suppresion of food consumption and body weight gain
800 mg/kg bw: Clinical signs:signs of loss of general conditions like piloerection and soiled fur;
Supression of food consumption and body weight gain; hematology findings: decrease in the hematocrit value, reticulocyte count and platelet count; reduced chlorid levels, calcium elevated and sodium reduced.
Necropsy: cecum was distended;increase in relative kidney weights
No abnormalities were detected: heart, pancreas, stomach, small intestine, large intestine, urinary bladder, ovary, pituitary, thyroid, parathyroid, spleen, lymph node, bone marrow or brain
Remarks: Histopathological changes in the kidney tended to recover and the other changes related to the test substance disappeared after 14 -day recovery period.
The authors consider: NOAEL: 80 mg/kg bw/day for both sexes
*GPT: serum glutamic pyruvic transaminase (synonym: alanine aminotransferase ALAT).
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- NOAEL
- 80 mg/kg bw/day
- Study duration:
- subacute
- Species:
- rat
- Quality of whole database:
- The endpoint is concluded based on a subacute toxicity study on CBS conducted to GLP and test guideline (equiv. OECD 422). It has been evaluated to be of good quality (Klimisch score = 2). Further supporting evidence is available from a supporting study of good quality (Klimisch score 2) conducted to sound scientific principle.
A recent scientific evaluation of the Sulfenamides category data set was undertaken to identify testing requirements to ensure suitable coverage of required endpoints for all member substances. This category member has been identified as having a data gap for sub-chronic repeated dose toxicity testing (OECD 408), required in accordance with Annex IX of Regulation (EC) No 1907/2006. A testing proposal has been submitted for an OECD 408 on CBS via the oral route (January 2023). The results of this study will be used to complete the dossier for CBS, as well as form part of the overall category data set as a source study for read across.
Repeated dose toxicity: dermal - systemic effects
Link to relevant study records
- Endpoint:
- short-term repeated dose toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, comparable to guideline study.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 410 (Repeated Dose Dermal Toxicity: 21/28-Day Study)
- Deviations:
- no
- Principles of method if other than guideline:
- In a 21-day dermal toxicity study 5 male and 5 female rabbits were treated with 0, 125, 500 or 2000 mg Santocur/kg/day (5 days/weeks) on intact and abraded skin. The animals were observed for mortality, clinical signs, body weight gain and dermal irritation. A gross pathology and microscopic examination was performed at the end of the study.
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- Route of Administration: dermal
- Duration of treatment / exposure:
- 21 d
- Frequency of treatment:
- Daily (5 days per week).
- Remarks:
- Doses / Concentrations:
125, 500 or 2000 mg/kg bw/d
Basis: - No. of animals per sex per dose:
- Ten per dose and sex (5 per dose and sex intact skin, 5 per dose and sex abraded skin).
- Control animals:
- yes
- Details on study design:
- Post-exposure period: no data
- Positive control:
- None.
- Observations and examinations performed and frequency:
- Mortality, general observations, body weight gain, dermal irritation .
- Sacrifice and pathology:
- Gross pathology and microscopic examination.
- Clinical signs:
- no effects observed
- Description (incidence and severity):
- No test article-related signs were noted for any animal in the test groups; a few rabbits in the 125 and 500 mg/kg bw test groups exhibited some signs of occular irritation such as injected iris, corneal opacity and conjunctivial redness and swelling. None of the animals in the 2000 mg/kg group were noted for ocular irritation.
- Dermal irritation:
- no effects observed
- Description (incidence and severity):
- The majority of the rabbits in both control and test groups (abraded and intact skin) exhibited no dermal irritation during the study period; however during the study period a few rabbits in each CBS dose groups exhibited very slight to slight erythema on the application area of the skin, which was reversible within day 21 of the study; the incidence of very slight to slight desquamation was increased in test compound treated groups; but a dose-response relationship was not noticed.
- Mortality:
- no mortality observed
- Description (incidence):
- No test substance related mortality.
- Body weight and weight changes:
- no effects observed
- Description (incidence and severity):
- No statistically significant differences were seen.
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- effects observed, treatment-related
- Haematological findings:
- no effects observed
- Description (incidence and severity):
- No changes considered to be related to CBS were seen in the hematologic studies and biochemical studies.
- Clinical biochemistry findings:
- no effects observed
- Description (incidence and severity):
- No changes considered to be related to CBS were seen in the hematologic studies and biochemical studies.
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Description (incidence and severity):
- The body weight means of the different test groups and most of the mean organ weights were simular to the control groups.
- Gross pathological findings:
- no effects observed
- Description (incidence and severity):
- No findings related to the test substance were observed.
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- no effects observed
- Description (incidence and severity):
- The main microscopic findings in the skin were slight hyperkeratosis abd acanthosis in the epidermis and infiltration of inflammatory infiltrate in the dermis. These changes were present in control animals and CBS treated animals and were considered as unrelated to the application of CBS.
- Histopathological findings: neoplastic:
- not examined
- Other effects:
- no effects observed
- Key result
- Dose descriptor:
- NOAEL
- Effect level:
- 2 000 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: No major signs of local or systemic toxicity noted in rabbits treated with CBS at dosage of 125, 500, or 2000 mg/kg bw/day
- Key result
- Critical effects observed:
- no
- Conclusions:
- No evidence of toxicity related to test material administration.
- Executive summary:
In a dermal 21-day toxicity study comparable to OECD TG 100 CBS was applied daily to the intact and abraded skin in doses of 125, 500 and 2000 mg/kg bw and day to each of the male and female adult New New Zealand white rabbits for a period of 3 weeks. Saline was used as negative control. Ground CBS was applied onto the skin moistened and covered with occlusive dressing. No data were available indicating the administered concentrations or surface area doses of CBS. The exposure time was 6 hours per day for a 5-day per week basis, for a period of 21 consecutive days. Two rabbits died during the course of study. One male control rabbit was found dead on day 12 and one male treated with 2000 mg/kg bw and day on day 13, respectively. Neither death was attributed to treatment with CBS. No dose dependent effects were noted for general appearance, behaviour, body weight, haematology, blood biochemistry, gross pathology, absolute and relative organ weights and histopathology. During the study period a few rabbits in each of the CBS dose groups exhibited very slight to slight erythema on the application area of the skin, which was reversible within day 21 of the study. The incidence of very slight to slight desquamation seen as slight scaling was increased in animals of the CBS-dose groups when compared to controls. But a dose-response relationship was not noticed. At microscopy of the skin slight hyperkeratosis and acanthosis in the epidermis and infiltration of inflammatory infiltrate in the dermis were observed. These findings were present in both CBS-treated animals and those of controls. Their incidence and severity did not distinguish CBS-treated rabbits from controls. Therefore, the NOAEL for systemic effects and local effects in rabbits after repeated dermal exposure was 2000 mg/kg bw and day (Monsanto Co.1981).
Reference
No test article-related signs were noted for any animal in the test groups; a few rabbits in the 125 and 500 mg/kg bw test groups exhibited some signs of occular irritation such as injected iris, corneal opacity and conjunctivial redness and swelling. None of the animals in the 2000 mg/kg group were noted for ocular irritation.
mortality: control 1/10 males (on day 12); 1/10 males 2000 mg/kg (on day 13)
mortality not test substance related
Dermal Irritation:
the majority of the rabbits in both control and test groups (abraded and intact skin) exhibited no dermal irritation during the study period; however during the study period a few rabbits in each CBS dose groups exhibited very slight to slight erythema on the application area of the skin, which was reversible within day 21 of the study; the incidence of very slight to slight desquamation was increased in test compound treated groups; but a dose-response relationship was not noticed.
Body weight: no statistically significant differences were seen.
Clinical laboratory tests:
No changes considered to be related to CBS were seen in the hematologic studies and biochemical studies.
Gross pathology: No findings related to the test substance were observed.
Organ weights: the body weight means of the different test groups and most of the mean organ weights were simular to the control groups.
Histopathology: the main microscopic findings in the skin were slight hyperkeratosis abd acanthosis in the epidermis and infiltration of inflammatory infiltrate in the dermis. These changes were present in control animals and CBS treated animals and were considered as unrelated to the application of CBS.
Findings on the internal organs were consistent with the usual spontaneous lesions found in this strain of rabbits
No evidence of toxicity related to test material administration.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- NOAEL
- 2 000 mg/kg bw/day
- Study duration:
- subacute
- Species:
- rabbit
- Quality of whole database:
- The available study is GLP and assigned high quality (Klimisch 1)
Repeated dose toxicity: dermal - local effects
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Additional information
Repeated dose toxicity: Oral
The repeated dose toxicity of CBS was evaluated in both feed and gavage studies. These are summarised below.
1. MHWJ 1997: Gavage Subacute toxicity study (supp, K2)
In a subacute toxicity study equivalent to OECD 422 male and female rats (6 per dose and sex) were administered with 0, 25, 80, 250 and 800 mg/kg bw/day in sesame oil vehicle for 28 days and were sacrificed on day 29 (MHJW, 1997). Additional control and 800 mg/kg bw/day groups were treated for 28 days and then kept for a recovery period of 14 days before sacrifice on day 43. Data on hematology, clinical biochemistry, organ weights and incidences of histopathological findings were provided.
No mortality occurred during the study period. After administration of 800 mg/kg bw/day signs associated with loss of general condition like piloerection and solid fur were observed in female rats. Suppression of food consumption and body weight gain was observed in females given ≥250 mg/kg bw/day (significant p=0.01 at 800 mg/kg bw/day) and in males at 800 mg/kg bw/day.
Hematology revealed statistically significant shortening of the prothrombin time in males given = 250 mg/kg bw/day, and statistically significant decrease in hematocrit value, reticulocyte count and platelet count for the 800 mg/kg bw/day females.
Clinical biochemistry revealed statistically significant decreases in alanine aminotransferase (ALAT) for males of all CBS treated groups and for females given ≥ 80 mg/kg bw/day, in total protein for males treated at ≥ 250 mg/kg bw and day, in chloride levels in males and females at 800 mg/kg bw and day and in sodium levels in females at 800 mg/kg bw/day. In addition, calcium concentration was elevated in females at the 800 mg/kg bw/day dose group. Urinalysis revealed an increase of ketone bodies in males ≥ 250 mg/kg bw/day.
The statistically significant increase in relative kidney weights in the 800 mg/kg bw/day in males was considered to be associated with an increase in deposition of hyaline droplets in the proximal tubular epithelium of the kidneys in male rats evident at 250 and 800 mg/kg bw/day. This effect in male kidney showed a clear tendency towards reversibility. All other changes were completely reversible after the 14-day recovery period.
In summary, CBS-related effects were present in male and females Crj: CD (SD) rats ≥ 250 mg/kg bw/day. There were signs of coagulopathy of the blood in both sexes and effects in the kidney of male rats.
Based on these findings, a NOAEL of 80 mg/kg bw/day was assigned for both sexes. Suppression of food consumption and body weight gain were observed in doses of and above 250 mg/kg bw/d (LOAEL). Increase in relative kidney weight associated with an increase in deposition of hyaline droplets in the proximal tubular epithelium of the kidneys in male rats evident at and above 250 mg/kg bw/day. Notably, other than an increase in deposition of hyaline droplets (often associated with adult male rat specific α2u-globulin accumulation (Hamamura et. al., 2017)) there were no other particularly strong indications of adversity in the kidneys of male animals. This is supported by the tendency toward reversibility in satellite group animals.
2. Monsanto 1980: Dietary Subacute toxicity study (supp, K2)
In a dose range finding subacute feeding study, 5 male and 5 female Sprague Dawley rats received CBS in doses of 100, 250, 500, 1000 or 3000 mg/kg bw/day (corresponding to mean compound consumption of 0, 101, 249, 500, 961 and 3208 mg/kg bw/day in males; and 0, 92.5, 229, 459, 910 and 2769 mg/kg bw/day in females).
All males survived, whereas three of five females died at 3000 mg/kg bw/day (two deaths occurred during study week 2, 1 death occurred during study week 4). At 3000 mg/kg bw hair loss, labored breathing, yellowish material on the anogenital region and distention of the abdomen were noted. Significantly lower body weights were recorded in a dose-dependent manner at 500, 1000 and 3000 mg/kg bw/day (m: 15.5% - 72.8%, f: 20.7% - 57.1%). A slight body weight decrease were noted in males of dose group 100, 250 mg/kg bw (5.5 % and 4.8 % vs. control, respectively). Mean food consumption were significantly lower (18 - 62%) at 500, 1000 and 3000 mg/kg bw/day. A slight decrease in food consumption was also seen at 100 (m:5.8 %, f:4.9 %) and 250 mg/kg bw (m: 8 %, f: 7.6 %) compared to control. No compound-related gross necropsy lesions were seen in any of the treated rats that were either sacrificed at termination or died during the course of study. A NOAEL of 250 mg/kg was found, based on significant reduced body weight gain and food consumption at higher doses (LOAEL 500 mg/kg bw/day). The lack of blood biochemistry, haematology and histopathology data diminishes the validity of this NOAEL through food consumption and body data are generally as sensitive indicators of systemic toxicity.
3. NCI, 1968: Subchronic toxicity (supp, K3)
In a non-test guideline carcinogenicity study in B6C3F1 and B6AKF1 mice (18 males and 18 females), mice received a continuous daily oral application via gavage from 7th day of age until the mice were at weanling age (28 days) following which the compound was mixed with the ground feed for 18 months in total (ca.: 90 mg/kg bw/day, corresponding to a time-weighted average of 95.3 mg/kg bw/day). No adverse effects reported (NCI, 1968).
4. Testing proposal: OECD 408
A recent scientific evaluation of the Sulfenamides category data set was undertaken to identify testing requirements to ensure suitable coverage of required endpoints for all member substances. This category member has been identified as having a data gap for sub-chronic repeated dose toxicity testing (OECD 408), required in accordance with Annex IX of Regulation (EC) No 1907/2006. A testing proposal has been submitted for an OECD 408 on CBS via the oral route (January 2023). The results of this study will be used to complete the dossier for CBS, as well as form part of the overall category data set as a source study for read across.
Repeated dose toxicity: inhalation
In a 28-day inhalation toxicity study groups of Sprague-Dawley CD rats (10/sex/group) were whole body exposed to atmospheric concentrations of CBS of 0.0043, 0.0144 and 0.048 mg/l (analytic values which represent ca. 9 % of the nominal values) for 6 hours per day and 5 days per week for a period of four consecutive weeks (Monsanto 1981). The equivalent aerodynamic diameter of the CBS dust generated was 7.6 µm with a geometric standard deviation of 2.7 µm. This distribution of CBS particle sizes was above the recommendations in current inhalation guideline tests. Two further groups of rats (10 per sex) acting as control groups were exposed to compressed filter air alone. There were no treatment-related premature deaths. The rats exhibited occasional nasal irritation which appeared to be concentration related in term of severity and number of animals exhibiting the sign (no more data). This finding was observed immediately after the 6-hour exposure period, and disappeared by the next morning. Since the animals recovered from this lesion within 24 hours and these findings could not be correlated to histopathologic effects, the observation of nasal irritation in few CBS-exposed animals was not considered to be toxicologically adverse. No concentration dependent effects were noted for general appearance, behaviour, body weight, food consumption, haematology, urinalysis, gross pathology and absolute and relative organ weights. Clinical biochemistry revealed concentration-related statistically significant increase of serum glutamic oxaloacetic transaminase (SGOT) values in males and females exposed to 0.0144 and 0.048 mg/l compared to control groups. However, these findings were not confirmed by microscopic changes in the liver and should therefore not be regarded as an adverse effect. In addition, there were no changes in organ weights and macroscopic examination that were considered to be an effect of exposure to CBS. A slight increased incidence of brown pigment within sinusoidal macrophages in lymph nodes was present in 2/10 males and 4/10 females exposed to 0.048 mg/l compared to controls (3/20 females). Very slight to slight increase of hemosiderin storage in the spleen was observed in 5/10 females of the 0.048 mg/l concentration group. This finding was not observed in any of the 20 males and females of the internal controls. The author suggested that these lesions may represent a compound effect; however similar lesions were commonly observed in control rats at this facility and therefore the possibility that they were spontaneous in origin cannot be excluded. Microscopic finding was present in the eyes of a few animals exposed to the highest concentration of 0.048 mg/l. There was a slight increased incidence and severity of conjunctivitis in each of 2/10 males and females when compared to control (1/20 males). Because a limited number of animals were affected this finding was considered to be incidental and spontaneous nature. Microscopy of nasal turbinates, olfactory bulb and the lungs showed a number of histopathologically findings. Their type, incidence and severity did not distinguish CBS-exposed rats from controls. Based on the fact that the signs of nasal irritation observed at clinical examination were only short in duration and could not be correlated to histopathological effects, no toxicological significance was attached to this finding.
In summary, the study is limited concerning the recommended particle-size distribution given in current guidelines. To allow for exposure of all relevant regions of the respiratory tract, aerosols with mass median aerodynamic diameters (MMAD) ranging from 1 to 3 µm are recommended. The MMAD used was above the recommendations given (7.6 µm) and therefore, a limited systemic exposure seems reasonable. Thus, the findings of this study are questionable, especially for systemic effects. Whereas the local effects on the respiratory tract noted should be used as supporting evidence.
Repeated dose toxicity: dermal
In a dermal 21-day toxicity study comparable to OECD TG 410 CBS was applied daily to the intact and abraded skin in doses of 125, 500 and 2000 mg/kg bw and day to each of the male and female adult New Zealand white rabbits for a period of 3 weeks. Saline was used as negative control. Ground CBS was applied onto the skin moistened and covered with occlusive dressing. No data were available indicating the administered concentrations or surface area doses of CBS. The exposure time was 6 hours per day for a 5-day per week basis, for a period of 21 consecutive days. Two rabbits died during the course of study. One male control rabbit was found dead on day 12 and one male treated with 2000 mg/kg bw and day on day 13, respectively. Neither death was attributed to treatment with CBS. No dose dependent effects were noted for general appearance, behaviour, body weight, haematology, blood biochemistry, gross pathology, absolute and relative organ weights and histopathology. During the study period a few rabbits in each of the CBS dose groups exhibited very slight to slight erythema on the application area of the skin, which was reversible within day 21 of the study. The incidence of very slight to slight desquamation seen as slight scaling was increased in animals of the CBS-dose groups when compared to controls. But a dose-response relationship was not noticed. At microscopy of the skin slight hyperkeratosis and acanthosis in the epidermis and infiltration of inflammatory infiltrate in the dermis were observed. These findings were present in both CBS-treated animals and those of controls. Their incidence and severity did not distinguish CBS-treated rabbits from controls. Therefore, the NOAEL for systemic effects and local effects in rabbits after repeated dermal exposure was 2000 mg/kg bw and day (Monsanto Co.1981).
In conclusion:
Oral
The 28-day gavage study with Crj: CD (SD) rats (MHWJ 1997) was considered to be the most relevant oral toxicity study for NOAEL determination. In this subacute toxicity study signs of coagulopathy of the blood were observed in both male and female rats. In males shortening of the prothrombin time (statistically significant) was noted after oral administration of = 250 mg/kg bw and day CBS, and in females statistically significant decreased platelet count was noted at 800 mg/kg bw and day. CBS-related effects were present in male and female Crj: CD (SD) rats at = 250 mg/kg bw and day. There were signs of a coagulopathy of the blood in both sexes and effects in the kidney of male rats. No relevant systemic effects were observed in male and female rats after repeated oral administration of 80 mg/kg bw and day CBS. Thus, the NOAEL is considered to be 80 mg/kg bw and day.
Inhalation
The inhalation toxicity of CBS was examined in a subacute inhalation study (Monsanto Co. 1981). The study is limited concerning the recommended particle-size distribution given in current guidelines and thus an exposure of all relevant regions of the respiratory tract might not have been given. The biologically relevance of sporadic findings reported, especially systemic effects are questionable. In light of these limitations the data from the oral subacute toxicity study (NOAEL: 80 mg/kg bw and day, MHWJ 1997) will be used for calculation of the systemic DNEL; whereas local effects noted in the inhalation study will be used as supporting evidence. Based on the fact that signs of nasal irritation observed at clinical examination were reversible, only short in duration and could not be correlated to histopathological effects, no toxicological significance was attached to this finding, and thus, no relevant local effects were observed up to 0.048 mg/l.
Dermal
In a subacute dermal toxicity study using doses of 125, 500 and 2000 mg/kg bw and day no systemic or local effects were noted in New Zealand White rabbits after repeated dermal exposure for three weeks with 2000 mg/kg bw and day (Monsanto 1981). Thus, the NOAEL is considered to be 2000 mg/kg bw and day.
References:
Hamamura M, Oshikata T, Katoku K, Tsuchitani M, Yamaguchi R. Two types of deposits, hyaline droplets and eosinophilic bodies, associated with α2u-globulin accumulation in the rat kidney. J Toxicol Pathol. 2017 Oct;30(4):275-282. doi: 10.1293/tox.2017-0023. Epub 2017 Jul 8. PMID: 29097837; PMCID: PMC5660949.
Justification for classification or non-classification
Harmonised classification:
The substance has no harmonised classification according to the Regulation (EC) No. 1272/2008 (CLP).
Self-classification:
Based on the current dataset, no classification is required according to the classification criteria of Regulation (EC) No. 1272/2008 (CLP). None of the category members is classified for repeated dose toxicity. An OECD 408 study is planned on CBS. The dossier will be updated as soon as the new study becomes available.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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