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EC number: 200-589-6 | CAS number: 64-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Not a standard stuyd, no LC50 is calculated, the exposure duration is too short (4 h max) and effects are observed 1 to 3 weeks after exposure. This species is filamentous.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of Alkylating Agents on Cladophora crispata (Roth.) Kuetz.
- Author:
- Aruna M , Vidyavati
- Year:
- 2 008
- Bibliographic source:
- vegetos 21(1): 105-119
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Exposure for 1 to 4 hours. Observations 1 to 3 weeks after exposure. Determination of survival, dry weight, chlorophyll content.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Diethyl sulphate
- EC Number:
- 200-589-6
- EC Name:
- Diethyl sulphate
- Cas Number:
- 64-67-5
- Molecular formula:
- C4H10O4S
- IUPAC Name:
- diethyl sulfate
- Details on test material:
- no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Cladophora crispata
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 4 h
Results and discussion
Effect concentrations
- Remarks on result:
- other: no calculation performed
Any other information on results incl. tables
The percentage of healthy cells was lower compared to control in all the DES treated cultures which progressively increased by second week in 0.0001%. But, 0.01% concentration treated cultures showed decrease in their survival. In samples treated with higher concentration (0.1%) and longer duration (4 hours) the filaments of the alga could not survive up to third week and the cells were unhealthy and showed brown chloroplast. The percentage of damaged cells increased with increasing concentrations and durations of the diethyl sulphate treatment. The dry weight values increased gradually from first to third week in the control samples. There was a dose-dependent decrease of the dry weight and chlorophyll content in DES exposed cells. 0.01%, and 0.1% concentrations treated for 4 hours duration, the cultures showed completely decreased values in the chlorophyll content.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Effects of DES were observed on this alga but no LC50 or NOEC is available.
- Executive summary:
The algal material selected for the test was Cladophora crispata (Roth) Kuetz. Diethyl sulphate was tested in various percentage concentrations (0.0001, 0.001, 0.01 and 0.1) for duration of 1, 2 and 4 hours for a period of three weeks. Observations were recorded with reference to survival percentage, dry weight, chlorophyll content, morphological and cytological variations.
Effects of DES were observed on this alga but no LC50 or NOEC is available.
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