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EC number: 283-044-5 | CAS number: 84539-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-11-30 to 1996-04-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Since the test substance was soluble in water, a stock solution of 15.4 mg FeEDDHMANa per mL was prepared by adding quantitatively 7.71g of test substance to 500 mL of milli-RO water. This stock solution was used for the preparation of the test concentration of 160 and 1600 mg/kg dry mass. For preparation of the lower concentrations the stock solution was diluted 10 times. An amount of the formulation and deionised water were added to artificial soil (containing 5% water).
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm, Eisenia foetida foetida
- Source: Notox B.V. facilities
- Age at test initiation (mean and range, SD): two months
- Weight at test initiation (mean and range, SD): 300-570 mg
- Feeding: No feeding from 24 hours prior to the test and during the total test period
ACCLIMATION
- Acclimation period: 1 day prior to the test - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 18 to 22 °C
- pH:
- 5.7 - 6.3
- Moisture:
- 37.5%
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): approximately 1 litre, all glass, covered with a plastic lid
- Amount of soil or substrate:
- No. of organisms per concentration: 40 earthworms in the control and highest concentration, 10 earthworms per concentration in between
TEST MEDIUM
Artificial soil as described in the guideline:
- 10% sphagnum peat (Jongkind B.V., Aalsmeer, The Netherlands);
- 20% kaolin clay (Silex Pottenbakkermat., 's-Hertogenbosch, The Netherlands);
- approx. 69% industrial sand M32 (Gamma, 's-Hertogenbosch, the Netherlands).
pH was adjusted to 6.0 +/- 0.5 by addition of calcium carbonate
OTHER TEST CONDITIONS
- Light intensity: 500-550 lux - Nominal and measured concentrations:
- nominal: 0.16, 1.6, 16, 160 and 1600 mg/kg dry mass of artificial soil
- Reference substance (positive control):
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 1 600 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: No test substance related mortality or other effects to the earthworms were recorded at any of the concentrations tested throughout the whole 14-day period of exposure. One worm was missing (presumably dead) in vessel D of the highest concentration at the end of the test, but up to 10% spontaneous mortality is acceptable.
- Total mass of adults at beginning of test: In the control the reduction in mean earthworm mass was 14%. There was a reduction in mean earthwormmass during the test at all concentrations, which can be explained by the absence of food during the exposure period. The reduction was not test substance related. - Reported statistics and error estimates:
- Not performed
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study the test substance did not induce significant acute mortality of Eisenia foetida foetida at or below 1600 mg/kg dry soil after 14 days of exposure.
- Executive summary:
FeNa-EDDHMA was investigated according to the OECD Guideline 207 on Earthworm Acute Toxicity Test.
Earthworms of the species Eisenia foetida foetida were exposed to 5 concentrations of the test item in medium for 14 days. Milli-RO water (tap water purified by reverse osmosis) was used to dissolve the test substance, before mixing it with the artificial soil. 40 Earthworms were exposed to the blank and the highest concentration, i.e. 10 per vessel, and 10 earthworms were exposed to the concentrations in between. The concentrations of the test substance in the final study were nominally 0.16, 1.60, 16, 160 and 1600 mg/kg dry mass of artificial soil (further referred to as dry soil).
No test substance related mortality or other effects to the earthworms were recorded at any of the concentrations tested throughout the whole 14-day period of exposure. There was a reduction in mean earthworm mass during the test at all concentrations, which can be explained by the absence of food during the exposure period. The reduction was not test substance related. In the control, no earthworms died or showed other effects during the total exposure period of 14 days. The average loss of biomass of the earthworms in the controls did not exceed 20%.
Finally, under the conditions of the present study the test substance did not induce significant acute mortality of Eisenia foetida foetida at or below 1600 mg/kg dry soil after 14 days of exposure (NOEC).
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Remarks:
- A short-term toxicity test was performed and is considered to be sufficient to cover the endpoint, since the substance will not persist in soil due to its physico-chemical properties (high water solubility, low log Kow and log Koc)..
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Read across justification is attached in chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 1 600 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Referenceopen allclose all
Description of key information
LC50(14d) > 1600 mg/kg soil dw (nominal) for mortality of Eisenia foetida (OECD 207, read across)
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity of Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts (CAS No. 84539-55-9) to soil macroorganisms are available, the assessment was based on a study conducted with source substance N,N'-Bis(2-hydroxy-4-methylphenyl)ethylenediamine diacetic acid, ferric-sodium complex (CAS No. 149057-66-9) in a read across approach. This is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification for suitability of read across between target and source substance is given in the read across justification document attached to chapter 13. Short-term toxicity tests are considered sufficient to cover the endpoint, since the sustance is a) highly soluble in water, b) the logPow and adsorption potential is low and c) the appropriate assessment factor was used for the environmental risk assessment.
The key study was performed according to OECD guideline 207 and GLP using Eisenia foetida as test organism (Van Erp 1995). Concentrations between 0.16 and 1600 mg/kg dw soil were tested in artificial soil over an exposure time of 14 days. The test substance did not induce mortality up to the highest concentration tested. Thuis, the 14d-LC50 is determined to be > 1600 mg/kg dry soil. Based on the suitability of the read across approach, this conclusion is also considered to be true for Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts (CAS No. 84539-55-9).
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