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Diss Factsheets
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EC number: 219-291-2 | CAS number: 2403-88-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Measured by gas chromatography 2 - 3 times a week (before and after the replacement of the test water.)
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Supplied by NIES (National Institute of Environmental Studies) of Japan.
- Age at study initiation: Juveniles within 24 h old.
- Control group: Yes. - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
- Hardness:
- 228 - 236 mg/L as CaCO3
- Test temperature:
- 19.2 - 20.7 °C
- pH:
- pH = 7.1-9.5
- Dissolved oxygen:
- DO = 8.0 - 8.6 mg/L
- Nominal and measured concentrations:
- 0, 3.7, 6.7, 12.0, 21.6, 38.9, 70.0 mg/L
- Details on test conditions:
- Test conditions
- Stock solutions preparation and stability: No solvent used. Test chemical was diluted to 0.3 wt.% with dilution water (Elendt M4) and it was diluted with the dilution water for testing.
- Exposure vessel type: A 80 ml test solution in a 100 ml glass beaker; 10 beakers per treatment.
- Dilution water source: Dechlorinated tap water
- Lighting: < 1,200 lux, 16 h : 8 h light-dark cycle
- Water chemistry in test: ;
- Feeding: Chlorella vulgaris, 0.1 - 0.2 mgC/day/individual
- Element (unit) basis: Mean cumulative numbers of juveniles produced per adult (reproduction)
- Number of replicate, individuals per replicate: Ten replicates, One daphnia per replicate concentrations: 0, 3.7, 6.7, 12.0, 21.6, 38.9 and 70.0 mg/L, because 48 h-EiC50 for parent Daphnia (Acute immobilisation test) was 100.0 mg/L.
- Method of calculating mean measured concentrations: Geometric mean.
- Exposure period: 21 day
- Analytical monitoring: By GC analysis. A 88.9 - 105.4 % of the nominal concentration at preparation; 84.7 - 118.9 % just before the renewal of the test water (after 2 days exposure). - Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 and EC50 values after 21 days exposure were more than 70 mg/L and 46.2 mg/L (38.6 - 57.5 mg/L) based on the nominal concentration. The NOEC and LOEC for reproduction rate were evaluated to be 3.7 mg/L and 6.7 mg/L, respectively. Experimental designs and results were well documented and prescribed conditions in the guideline were well satisfied.
Reference
Results of analytical monitoring by GC analysis: A 88.9 - 105.4 % of the nominal concentration at preparation; 84.7 - 118.9 % just before the renewal of the test water (after 2 days exposure).
- LC50 for parental Daphnia (21 d) : > 70.0 mg/L
- EC50 (21 d, reproduction) : 46.2 mg/L
Description of key information
The NOEC (21 d) regarding the reproduction is 3.7 mg/L. The No Observed Effect Concentration is based on nominal concentration as the measured concentration was within 80-120% of the analytical measured concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 3.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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