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EC number: 200-898-6 | CAS number: 75-75-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Methanesulphonic acid (MSA) is corrosive to the skin (pH value <2), public available information states LD50 oral between 300 and 2000 mg/kg bw and LD 50 dermal between 200 and 2000 mg/kg bw.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with GLP.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- other: EPA 40 CFR Part 792
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Inc., Portage, Michigan
- Weight at study initiation: 205.3 g mean (males), 204.7 g mean (females)
- Diet (e.g. ad libitum): PMI certified Rodent Chow #5002
- Water (e.g. ad libitum): tap water
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 38-61
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 0.38, 0.75, 1.13 ml/kg bw
MAXIMUM DOSE VOLUME APPLIED: 1.13 ml/kg bw - Doses:
- 500, 1000, 1500 mg/kg body weight
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations: no data; weighing: every 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, mortality, necropsy - Statistics:
- The LD50 was determined separately for both sexes using the method of Litchfield and Wilcoxon.
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 157.5 mg/kg bw
- 95% CL:
- 748 - 1 791.1
- Remarks on result:
- other: 70 % methane sulfonic acid
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 860.1 mg/kg bw
- 95% CL:
- 540.1 - 1 369.7
- Remarks on result:
- other: 70 % methane sulfonic acid
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 407.6 mg/kg bw
- 95% CL:
- 944.2 - 6 139.2
- Remarks on result:
- other: 70 % methane sulfonic acid
- Mortality:
- Males: 1/5, 3/5, and 4/5 deaths at 500, 1000, and 1500 mg/kg bw, respectively.
Females: 1/5, 0/5, and 3/5 deaths at 500, 1000, and 1500 mg/kg bw, respectively. - Clinical signs:
- other: The most notable clinical abnormalities observed during the study included salivation, breathing abnormalities, wobbly gait, decreased activity, decreased defecation, rough haircoat, urine/fecal stain and dark material around the facial area.
- Gross pathology:
- The most notable gross internal findings were observed in the animals that died and included abnormal content and reddened/thickened mucosa and discolouration in the digestive tract, dark red foci on the liver and blackish-purple spleens. No significant gross findings were observed at necropsy on day 14.
- Other findings:
- - Potential target organs: Digestive tract
Reference
Table: Mortalities during 14 days of observation period
Dose group (mg/kg bw) |
No. of animals |
Study day |
Mortality |
|||||||||||||
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13-14 |
|||
500 |
5 M |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1/5 |
1000 |
5 M |
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3/5 |
1500 |
5 M |
1 |
1 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4/5 |
500 |
5 F |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1/5 |
1000 |
5 F |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0/5 |
1500 |
5 F |
1 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3/5 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 157.5 mg/kg bw
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to OECD guideline with GLP, acceptable with restrictions.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- Limit dose: 1000 mg/kg
- Qualifier:
- according to guideline
- Guideline:
- other: 49 CFR 173.132
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Millbrook Breeding Labs, MA
- Weight at study initiation: males 2.5-3.1 Kg, females 2.8-3.0 Kg
- Housing: 1 per cage
- Diet (e.g. ad libitum): Fresh PMI rabbit chow
- Water (e.g. ad libitum): drinking water
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- % coverage: 10
- Type of wrap if used: no data
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Yes, distilled water
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5 ml/kg bw
- Concentration (if solution): 200 mg/ml
- Constant volume or concentration used: yes - Duration of exposure:
- 24 hours
- Doses:
- 1000 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical observations 1 and 2 hours postdose and once daily thereafter; dermal responses 24 hours postdose and on days 7 and 14; weighing pretest and at termination
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 1 000 mg/kg bw
- Mortality:
- No mortality occured, all animals survived the application of 1000 mg/kg bw Methane Sulfonic Acid.
- Clinical signs:
- other: Instances of few faces were the only abnormal physical signs noted during the observation period. Dermal responses were slight to well defined on day 1, absent to severe on day 7 and absent to slight on day 14.
- Gross pathology:
- Necropsy results were normal.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
Additional information
The substance is classified as corrosive to the skin. Therefore, in accordance with the REACH information requirements (column 2 of the REACH Annexes VII and VII) testing for acute toxicity does not need to be conducted.
Nevertheless, some reliable and relevant information on the acute toxicity is publically available (ElfAtochem1997/Elfatochem1998) stating LD50 values (oral-gavage) between 300 and 2000 mg/kg (even if corrected to active ingredient) bw in male and female rats applicating 70% MSA or anhydrous MSA. These acute oral effects were not observed when MSA was neutralised with NaOH (see 7.8.1, Chevron 2005).
Additionally information from a acute dermal toxicity study with rabbits according to OECD 402 shows no deaths up to and including 1000 mg/kg bw (MBRL 2002) but in a less reliable supporting dermal toxicity studies 2/3 animals where found death 24h after dermal application of 2000 mg/kg bw (Pennwalt 1978). Therefore a acute dermal toxicity category 4 ghs, eu is assumed.
Further Saturation concentration (1.88mg/m3) of MSA does not lead to acute toxic (systemic) effects in mice (Pennwalt 1976).
All these effects may also be attributed to the corrosive nature of the test substance therefore no further testing is suggested.Justification for classification or non-classification
Acute effects are due to the corrosive properties of MSA. These effects observed lead to some deaths in higher dosegroups. Thereforee MSA has to be labeled as follows:
EU classification according to Annex I of Directive 67/548/EEC: Xn; R21/22 Harmful in contact with skin and if swallowed.
GHS classification (GHS UN rev.3, 2009): CAT 4 (oral), H302: Harmful if swallowed and CAT 4 (dermal), H312: Harmful in contact with skin..
Furthermore due to the corrosive character which causes the toxic effect an irritation of respiratory system can not be excluded when inhaled. Therefore MSA is further classified as STOT SE 3, H335: May cause respiratory irritation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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