Formaldehyde, oligomeric reaction products with aniline

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: A GLP study carried out in accordance with an OECD Guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-methylenedianiline

EC Number:

202-974-4

EC Name:

4,4'-methylenedianiline

Cas Number:

101-77-9

Molecular formula:

C13H14N2

IUPAC Name:

4,4'-methylenedianiline

Details on test material:

4,4'-methylenedianiline (4,4'-MDA)
Uniformly [14C] ring labeled
Lot no CFQ8060, Amersham Life Science, Piscataway, New Jersey, USA.
Specific activity: 2.42 +/- 0.00 mCi/mmole (mean +/-1 std. Dev., n=3)
Radiochemical purity: 96.0 +/- 0.7% (mean +/- 1std. Dev., n=3)
Approx. chemical purity: 94.9% based on peak area percentage from HPLC-UV analysis.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Municipal activated sludge from the wastewater treatment plant Mannheim/Baden Württemberg (Germany) was collected on 06 July 2007 from the aeration tank of the plant and aerated in the laboratory until further use. A suitable aliquot of the activated sludge suspension was sieved using a finely woven mesh with a mesh size of about 1 mm. After settling, the supernatant was discarded and the sludge suspension was filled up with tap water. This washing procedure was repeated three times. The suspended solids concentration of the resulting sludge suspension will be determined, and a suitable portion was used to inoculate the mineral medium at a final suspended solids concentration of 30 mg/L. The inoculum was pre-adapted to the test conditions by stirring and aeration with CO2-free air at the test temperature for about five days. After this adaptation period, the inoculated mineral medium (1.5 L) was dispensed into the various test vessels.

Reason for the choice of the biological test system: The test guideline recommends the usage of activated sludge from a wastewater treatment plant, treating municipal sewage.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

ca. 500 µg/L

Based on:

other: corresponding to a total radioactivity of about 11 microCi in the test assay.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

radiochem. meas.

Details on study design:

Test temperature 22+/-2 degree C

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

46

St. dev.:

3.96

Sampling time:

28 d

Details on results:

46% biodegradation after exposure of 28 days
53% (standard deviation +/-3.54%) after exposure of 63 days.

BOD5 / COD results

Results with reference substance:

66% degradation after 14 days (%CO2/ThCO2)

Any other information on results incl. tables

The test substance was partly or moderately biodegradable. The study was based on OECD Guideline 301B and measured the evolution of CO2 to determine the degree of biodegradation. Therefore, no BOD was determined.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: moderately biodegradable