Potassium sodium 3,3'-(3(or4)-methyl-1,2-phenylenebis(imino(6-chloro)-1,3,5-triazine-4,2-diylimino(2-acetamido-5-methoxy)-4,1-phenylenazo)dinaphthalene-1,5-disulfonate

Toxicity to reproduction

Administrative data

Endpoint:

one-generation reproductive toxicity

Remarks:

based on test type (migrated information)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Annex V (1-generation reproduction study)

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Potassium sodium 3,3'-(3(or4)-methyl-1,2-phenylenebis(imino(6-chloro)-1,3,5-triazine-4,2-diylimino(2-acetamido-5-methoxy)-4,1-phenylenazo)dinaphthalene-1,5-disulfonate

EC Number:

403-810-6

EC Name:

Potassium sodium 3,3'-(3(or4)-methyl-1,2-phenylenebis(imino(6-chloro)-1,3,5-triazine-4,2-diylimino(2-acetamido-5-methoxy)-4,1-phenylenazo)dinaphthalene-1,5-disulfonate

Cas Number:

140876-13-7

Molecular formula:

C51H42Cl2N16O16S4

IUPAC Name:

3-{2-[4-({4-chloro-6-[(2-{[4-chloro-6-({4-[2-(4,8-disulfonaphthalen-2-yl)diazen-1-yl]-5-acetamido-2-methoxyphenyl}amino)-1,3,5-triazin-2-yl]amino}-5-methylphenyl)amino]-1,3,5-triazin-2-yl}amino)-2-acetamido-5-methoxyphenyl]diazen-1-yl}naphthalene-1,5-disulfonic acid; 3-{2-[4-({4-chloro-6-[(2-{[4-chloro-6-({4-[2-(4,8-disulfonaphthalen-2-yl)diazen-1-yl]-5-acetamido-2-methoxyphenyl}amino)-1,3,5-triazin-2-yl]amino}-6-methylphenyl)amino]-1,3,5-triazin-2-yl}amino)-2-acetamido-5-methoxyphenyl]diazen-1-yl}naphthalene-1,5-disulfonic acid

Test animals

Species:

other: Rat (Alpk: ApfSD)

Administration / exposure

Route of administration:

oral: unspecified

Details on exposure:

Method of administration or exposure: In diet

Frequency of treatment:

Dosing regime (males): 7 days/weekr
Dosing regime (females): 7 days/week

No. of animals per sex per dose:

Male: 26 animals at 0 mg/kg or mg/l
Male: 26 animals at 104 mg/kg or mg/l
Male: 26 animals at 526 mg/kg or mg/l
Male: 26 animals at 2127 mg/kg or mg/l
Female: 26 animals at 0 mg/kg or mg/l
Female: 26 animals at 111 mg/kg or mg/l
Female: 26 animals at 552 mg/kg or mg/l
Female: 26 animals at 2304 mg/kg or mg/l

Results and discussion

Results: P0 (first parental generation)

Details on results (P0)

No adverse treatment-related effects were observed. Orange staining of the fur/skin was noted in most males and all females in the lowest dose group, and all animals in the mid-, and top dose groups. Eleven pairs of animals (including controls) failed to produce a litter following mating. These animals were re-mated during week 18 of the study; overall with the exception of 3 females and 1 male rat, all the Fo parents which failed to oroduce a litter after the first mating were proven to be fertile after re-mating.

Effect levels (P0)

Remarks on result:

not determinable

Results: F1 generation

Details on results (F1)

Effects on F1 generation:
No toxicologically significant treatment-related effects on litter size, survival, growth or development of the offspring were observed. Orange staining of the skin and fur was noted in all pups of both sexes at all dose levels of the test substance. No treatment-related effects on survival and growth occurred throughout lactation.

Effect levels (F1)

Remarks on result:

not determinable

Overall reproductive toxicity

Reproductive effects observed:

not specified

Any other information on results incl. tables

None.