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Diss Factsheets
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EC number: 258-132-1 | CAS number: 52722-86-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Remarks:
- Shin Nippon Biomedical Laboratories, LTD. (Japan)
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2,2,6,6-tetramethylpiperidin-4-ol
- EC Number:
- 219-291-2
- EC Name:
- 2,2,6,6-tetramethylpiperidin-4-ol
- Cas Number:
- 2403-88-5
- IUPAC Name:
- 2,2,6,6-tetramethylpiperidin-4-ol
- Details on test material:
- - Name of test material (as cited in study report): 2,2,6,6-tetramethylpiperidin-4-ol
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Japan, Inc
- Age at study initiation: 7 weeks old
- Weight at study initiation: 273-299g
- Acclimation period: 6 days
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: water
- Duration of treatment / exposure:
- 2 days
- Frequency of treatment:
- 2
- Post exposure period:
- 24 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 250, 500, 1000, 1500 mg/kg
Basis:
actual ingested
- No. of animals per sex per dose:
- 6
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
- Route of administration: oral: gavage
- Doses / concentrations: 20 mg/kg
Examinations
- Tissues and cell types examined:
- immature erythrocytes (IE)
micronucleated immature erythrocytes (MNIE)
erythrocytes - Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION: The bone marrow cells suspension was smeared onto a clean slide. The slides were stained with acridine orange solution.
METHOD OF ANALYSIS: The coded smear slides were observed under a microscope. - Statistics:
- The IE ratio, an indicator of suppression of bone marrow cell growth, was analyzed by Student's t-test. For the incidence of MNIE's, tables of Kastenbaum and Bowman were applied.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- 1 of 6 animals died at 1500mg/kg
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
The micronucleated immature erythrocytes (MNIE) % values of this substance treatment groups were within 0.08 to 0.12% and no significant difference was observed in comparison with the negative control group (0.07%).
The immature erythrocytes (IE) % values were 47.30% at 250 mg/kg, 45.47% at 500 mg/kg, 43.90% at 1000 mg/kg and 39.96% at 1500 mg/kg, respectively, and these displayed significant differences in comparison with the negative control group (50.87%).
MNIE % and IE % were 3.50% and 40.20%, respectively in the positive control group, and showed significant differences in comparison with the negative control group. The negative control incidences of MNIE among tests was within the range of the laboratory background data and positive control ones showed remarkable increase.
This chemical dose not induce micronuclei under the test conditions employed.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
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