2-nitro-4-(trifluoromethyl)benzonitrile

Surface tension

Administrative data

Endpoint:

surface tension

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 - 07 Jan 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2022

Report date:

2022

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 115 (Surface Tension of Aqueous Solutions)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP certifying authority: Hessisches Ministerium für Umwelt, Klimaschulz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany

Type of method:

OECD harmonised ring method

Test material

Test material information

Constituent 1

Reference substance name:

2-nitro-4-(trifluoromethyl)benzonitrile

EC Number:

212-298-1

EC Name:

2-nitro-4-(trifluoromethyl)benzonitrile

Cas Number:

778-94-9

Molecular formula:

C8H3F3N2O2

IUPAC Name:

2-nitro-4-(trifluoromethyl)benzonitrile

Results and discussion

Surface tension

Surface tension:

60.8 mN/m

Temp.:

20 °C

Conc.:

618 mg/L

Remarks on result:

other: 90 % of the saturation concentration. The concentrations of the two solutions were determined by HPLC analyses (results: 609 mg/L for Test Solution 1 and 627 mg/L for Test Solution 2).

Any other information on results incl. tables

Individual Results of the Determination of the Concentration by HPLC
For the determination of the concentration of the test solutions, 1 mL of each test solution was diluted to a volume of 50 mL with acetonitrile/water 50/50 (v/v). The HPLC data of the two solutions (Test solution 1 and Test solution 2) of the test item (HPLC dilution factor 50) are given in Table below.

Table HPLC data: determination of the test item concentration

Name	Amount Reference mg/L	Area mAU*min	Dilution Factor	Slope (C1)	Slope Used (C1)	Content mg/L	Mean mg/L
Reference-1 Dil.1	17.12	4.77151022		0.27870971
Reference-1 Dil.1	17.12	4.75541135		0.27776935	0.27823953
Testsolution 1 Dil 1		3.38748643	50		0.27823953	608.7356
Testsolution 1 Dil 1		3.39354051	50		0.27823953	609.8236	609.2796
Testsolution 2 Dil 1		3.49277380	50		0.27823953	627.6559
Testsolution 2 Dil 1		3.48503983	50		0.27823953	626.2661	626.9610
Water blank		not detected	50
Water blank		not detected	50

Results (rounded): 609 mg/L (Test solution 1)

627 mg/L (Test solution 2)

 

Slope = Peak Area (Reference)/ Amount Reference (mg/L)

Content (mg/L) = (Peak Area (Sample) x Dil. factor)/ Slope (Mean Group)

Slope (Mean Group) = Mean value of slope factors (Group)

 

Individual Results of the Measurement of the Surface Tension
The surface tension values of the two aqueous solutions of the test item were determined.  The measurement was carried out at 20 °C ± 0.5 °C.
A correction factor (Harkins & Jordan) is included. 
The age of the test solutions at the beginning of the measurement was 25.0 hours for the Test solution 1 and 25.5 hours for the Test solution 2. The age of the test solution is defined as the period of time from the preparation of the solution to the recording of the surface tension value. The measured data are given in Table below.

Table: Surface tension of test item, technical substance, measured data

	Experiment 1		Experiment 2
	Time [s]	σM [mN/m]	Time [s]	σM [mN/m]
1. measurement	188.4	60.67	188.7	59.46
2. measurement	235.6	60.44	235.0	59.46
3. measurement	281.6	60.50	282.7	59.41
4. measurement	326.9	60.51	330.4	59.41
5. measurement	373.5	60.51	377.8	59.43
6. measurement	419.8	60.53	425.6	59.43
7. measurement	466.5	60.52	473.3	59.40
8. measurement	513.3	60.50	519.9	59.42
9. measurement	560.2	60.51	567.2	59.40
10. measurement	607.2	60.52	614.1	59.39
Mean σM [mN/m]		60.52		59.42

 

Mean (experiments 1 and 2): σmean = 59.97 mN/m

Standard deviation: 0.8 mN/m

Relative standard deviation: 1.3 %

The mean value, multiplied by the calibration factor (F=1.0134) results in:
σ = σmean · F = 60.8 mN/m

 

Final Result

The surface tension of two aqueous solutions of the test item, technical substance, was determined using the OECD harmonized ring method according to the OECD Guideline 115 (1995) and European Commission Council Regulation (EC) No 440/2008, Annex, Part A, method A.5. (2008).
The solubility of the test item in water is below 1 g/L (0.731 g/L in distilled water at 20 °C). According to the OECD Guideline and the EC Regulation saturated solutions were prepared and then diluted to 90 % of the saturation concentration. The concentrations of the two solutions were determined by HPLC analyses
(results: 609 mg/L for Test Solution 1 and 627 mg/L for Test Solution 2). The surface tension of the aqueous solution of the test item, technical substance, was: σ = 60.8 mN/m at 20 °C.