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Diss Factsheets
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EC number: 251-646-7 | CAS number: 33703-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 967
- Report date:
- 1967
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Draize test
Guinea pigs are challenged with 10 injecitons given intracutaneously. The the test material is applied every other day or three times a week at a random area of the back clipped free of hair.
After 14 days of the last application, a challenge injection is given. Readings of the area, height, and color (redness) of the reaction is made after 24h of the injections.
Interpretation of the sensitizing potential is dependent on the observed effects of all animals. - GLP compliance:
- not specified
- Type of study:
- intracutaneous test
- Justification for non-LLNA method:
- The study was perfomed before 24 April 2002, the year the OECD guideline 429 was adopted.
Therefore, the local lymph node assay was not mandatory and has not been performed.
Test material
- Reference substance name:
- Bis(2-ethylhexyl) adipate
- EC Number:
- 203-090-1
- EC Name:
- Bis(2-ethylhexyl) adipate
- Cas Number:
- 103-23-1
- Molecular formula:
- C22H42O4
- IUPAC Name:
- bis(2-ethylhexyl) adipate
- Test material form:
- liquid
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male
- Details on test animals and environmental conditions:
- no data
Study design: in vivo (non-LLNA)
Induction
- Route:
- intradermal
- Vehicle:
- olive oil
- Concentration / amount:
- 0.1% / 0.05 mL first injection
0.1% / 0.1 mL (9 following injecitons) - Day(s)/duration:
- 10
Challenge
- No.:
- #1
- Route:
- intradermal
- Vehicle:
- olive oil
- Concentration / amount:
- 0.1% / 0.05 mL after 14 days
- Day(s)/duration:
- 1
- No. of animals per dose:
- 10
- Details on study design:
- DEHA was further tested for sensitisation in a Draize test with 10 male guinea pigs.
On the first day, 0.05 ml of a 0.1% DEHA solution was dosed intracutaneously in shaved back or side skin.
Furthermore, 0.1 ml of a 0.1% DEHA solution was dosed three times a week for a period of 3 weeks.
Olive oil was used as a carrier.
The intracutaneous challenge treatment after 14 days with 0.05 ml of the 0.1% DEHA solution did not indicate any sensitizing potential from DEHA - Challenge controls:
- no data
- Positive control substance(s):
- not specified
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.1%
- Total no. in group:
- 10
- Remarks on result:
- no indication of skin sensitisation
Any other information on results incl. tables
Ten male guinea pigs were treated with intracutaneous injections into the dorsal skin. On the first day, 0.05 ml of a solution containing 0.1% DEHA in olive oil. For the next 3 weeks there were injected 3 times a week with 0.1 ml of the same solution. After 14 days the intradermal challenge with 0.05 ml of the 0.1 % DEHA containing solution gave no indication for skin sensitizing properties.
The area and height of the reaction was measured 24 hours after the challenge dose. The average area and height of the reaction at challenge were smaller than during induction.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
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