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EC number: 238-238-4 | CAS number: 14302-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented study, which was conducted according to the Guideline for Screening Mutagenicity Testing of Chemicals (Japan).
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Principles of method if other than guideline:
- A positive control (benzo(a)pyrene) was used, however, the results of the positive control were not listed.
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32 copper
- EC Number:
- 205-685-1
- EC Name:
- 29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32 copper
- Cas Number:
- 147-14-8
- Molecular formula:
- C32H16CuN8
- IUPAC Name:
- [29H,31H-phthalocyaninato(2-)-kappa~2~N~29~,N~31~]copper
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study report): Phtalocyanine Blue, Pigment Blue-15
- Analytical purity: technical grade
Constituent 1
Method
- Target gene:
- The purpose of the in vitro chromosomal aberration test is to identify agents that cause structural chromosomal aberrations in cultured mammalian cells. Structural aberrations may be of two types, chromosome or chromatid.
Species / strain
- Species / strain / cell type:
- other: Chinese Hamster CHL cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction from the liver of rats, treated with phenobarbital and 5,6-benzoflavone.
- Test concentrations with justification for top dose:
- 0, 0.75, 1.5, 3 mg/ml with and without metabolic activation.
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Details on test system and experimental conditions:
- A Chromosomal Aberration Assay was conducted according to the Guideline for Screening Mutagenicity Testing of Chemicals (Japan).
4 different experimental protocols were conducted, as recommended in the Japanese Guideline:
- Continuous treatment for 24 h without S9-mix (24/24 -S9).
- Continuous treatment for 48 h without S9-mix (24/24 -S9).
- Pulse treatment for 6 h without S9-mix followed by harvesting at 24 h (6/24 -S9).
- Pulse treatment for 6 h with S9-mix followed by harvesting at 24 h (6/24 +S9).
All chromosome aberrations observed (chromatid and chromosome gap, chromatid break, chromatid exchange, chromosome break, exchange) were recorded as number of the respective effect per 100 cells (equates to %).
Positive control: Benzo(a)pyren
Plates per dose: 1
Results and discussion
Test results
- Species / strain:
- mammalian cell line, other: Chinese Hamster CHL cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: with metabolic activation: > 3 mg/ml, without metabolic activation: > 3 mg/ml
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- not specified
- Additional information on results:
- None of dose groups induced any chromosomal aberrations or polyploidy in CHL cells with or without S9-mix.
Any other information on results incl. tables
Table 1: Results of Chromosomal Aberration test (continuous treatment for 24 h and 48 h without S9-mix):
|
Treatment time (h) |
Concentration (mg/ml) |
Number of cells |
Polyploid |
No. of chromosomal aberrations |
No. of cells with Chr. Ab. Incl. Gaps |
|||||
|
|
|
|
|
Chromatid type |
Chromosome type |
others |
|
|||
|
|
|
|
|
g |
ctb |
cte |
csb |
cse |
|
|
DMSO |
24 |
0 |
100 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
|
48 |
0 |
100 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
Test substance |
24 |
0.75 |
100 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
|
24 |
1.5 |
100 |
3 |
0 |
1 |
0 |
0 |
0 |
0 |
1 |
|
24 |
3.0 |
100 |
2 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
|
48 |
0.75 |
100 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
48 |
1.5 |
100 |
1 |
1 |
1 |
0 |
0 |
0 |
0 |
2 |
|
48 |
3.0 |
100 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Table 2: Results of Chromosomal Aberration test (pulse treatment for 6 h with and without S9-mix followed by harvesting at 24 h):
|
S9-mix |
Concentration (mg/ml) |
Number of cells |
Polyploid |
Chromosomal aberration (%) |
Number of cells with Chr. Ab. Incl. Gaps |
|||||
|
|
|
|
|
Chromatid type |
Chromosome type |
others |
|
|||
|
|
|
|
|
g |
ctb |
cte |
csb |
cse |
|
|
DMSO |
- |
0 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
+ |
0 |
100 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
Test substance |
- |
0.75 |
100 |
1 |
3 |
0 |
0 |
0 |
0 |
0 |
3 |
|
- |
1.5 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
- |
3.0 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
+ |
0.75 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
+ |
1.5 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
+ |
3.0 |
100 |
2 |
0 |
0 |
0 |
1 |
0 |
0 |
1 |
Abbreviations in table 1 and table 2:
g = chromatid and chromosome gap
ctb = chromatid break
cte = chromatid exchange
csb = chromosome break
cse = exchange
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.