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EC number: 202-377-9 | CAS number: 94-96-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: A standard protocol under GLP was used and results were found to be valid by peer review prior to publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-ethylhexane-1,3-diol
- EC Number:
- 202-377-9
- EC Name:
- 2-ethylhexane-1,3-diol
- Cas Number:
- 94-96-2
- Molecular formula:
- C8H18O2
- IUPAC Name:
- 2-ethylhexane-1,3-diol
- Test material form:
- liquid: viscous
- Details on test material:
- - Name of test material (as cited in study report): 2-ethyl-1,3-hexanediol (EHD)
- Molecular weight (if other than submission substance): 146.2
- Substance type: viscous liquid
- Physical state: liquid
- Analytical purity: 99.18%
- Impurities (identity and concentrations): 0.36% monbutyrate ester isomers, and < 0.10% each of other unidentified impurities.
- Stability under test conditions: stable
- Source: Union Carbide Co., Danbury, CT
Constituent 1
Method
- Target gene:
- histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium, other: TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 rat liver fraction from male Arochlor 1254-treated CD rats, obtained from Microbiological Associates (Bethesda, MD, USA).
- Test concentrations with justification for top dose:
- 0.3, 0.9, 2.8, 9.4 and 28 µl/plate, for both conditions of metabolic activation
- Vehicle / solvent:
- 100% ethanol
Controls
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene and 4-nitro-o-phenylenediamine
- Remarks:
- with and without S9 activation, respectively. Concentration was 10 µg/plate for both substances.
- Details on test system and experimental conditions:
- The plate incorporation method of Ames BN, McCann J and Yamasaki E, Mutation Research 31: 347, 1975, was used, with modifications according to Maron DM and Ames BN, Mutation Research, 113:173, 1983.
NUMBER OF REPLICATIONS:
NUMBER OF CELLS EVALUATED:
DETERMINATION OF CYTOTOXICITY: not reported. Cytotoxic concentrations were determined in preliminary dose-finding experiments, using triplicate plates/dose. Two of five doses were above 5000 µg/plate. - Evaluation criteria:
- A dose-related increase which was at least two-fold higher than the concurrent solvent control for two consecutive doses was the evaluation criteria for a positive result.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
2-Ethyl-1,3-hexanediol (EHD) was evaluated as negative (nongenotoxic) in the Ames assay. - Executive summary:
A bacterial reverse mutation assay was conducted on 2-ethyl-1,3-hexanediol (EHD), < 99% purity. The procedure used was according to standard protocols originally published by Ames BN, McCann J and Yamasaki E, 1975, and Maron DM and Ames BN, 1983. Five strains of Salmonella typhimurium were tested, TA 1535, TA 1537, TA 1538, TA 98 and TA 100, with and without metabolic activation (S9 rat liver fraction from male Arochlor 1254-treated CD rats, obtained from Microbiological Associates (Bethesda, MD, USA). Positive results were defined as a doubling of the background mutation rate, and positive controls were 2-aminoanthracene (10 µg/plate) without S9 and 4-nitro-o-phenylenediamine (10 µg/plate) with S9 activation. EHD was tested at concentrations up to cytotoxic levels, with and without S9 activation, with no increase in mutation rate. The substance is evaluated as nonmutagenic under conditions of this assay.
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