Glycerol, ethoxylated

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study which meets generally accepted scientific standards. No specification of test substance concentration. Limited documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The substance preparations were injected into the peritoneal cavity of mice. The mice were observed for 14 days and the LD50 was calculated.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Glycerol, ethoxylated

EC Number:

500-075-4

EC Name:

Glycerol, ethoxylated

Cas Number:

31694-55-0

Molecular formula:

(C2 H4 O)n (C2 H4 O)n (C2 H4 O)n C3 H8 O3 3n =>1-<6.5 mol EO

IUPAC Name:

Alkoxylation reaction product of glycerin as starter and ethylene oxyde (EO) as monomer

Details on test material:

- Name of test material (as cited in study report): Schmiermittel LK 2200
- pH 6.5 - 7.5
- Purity: 'technical' purity

Test animals

Species:

mouse

Strain:

other: NMRI Wiga

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male 28g, females 24g (mean weight)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Details on exposure:

The doses were administered as a 68.1% (w/v) preparation of the test substance in
aqua dest. as well as undiluted.

Doses:

10000 mg/kg bw
6810 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Several times on the application day, thereafter once each working day.
- Frequency of weighing: Body weight was determined before the start of the study only, as it was needed for determination of dose.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Mortality:

One male and one female mouse died on day 3 after injection (dose group 10000 mg/kg)

Clinical signs:

After application of the test substance: slight reduction of weight and unspecific symptoms (some animals: convulsion)

Body weight:

No further data

Gross pathology:

nothing abnormal detected

Other findings:

None

Any other information on results incl. tables

			# animals which died within
dose (mg / kg bw)	concentration	# animals	1h	24h	48h	7d	14d
10000	100%	5 male  5 female	0/5 0/5	0/5 0/5	0/5 0/5	1/5 1/5	1/5 1/5
6810	68,10%	5 male  5 female	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5	0/5 0/5