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EC number: 272-805-7 | CAS number: 68912-13-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 May 2000 - 30 May 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3a,4,5,6,7,7a-hexahydro-4,7-methano-1H-indenyl propionate
- EC Number:
- 272-805-7
- EC Name:
- 3a,4,5,6,7,7a-hexahydro-4,7-methano-1H-indenyl propionate
- Cas Number:
- 68912-13-0
- Molecular formula:
- C13H18O2
- IUPAC Name:
- Reaction mass of 3a,4,5,6,7,7a-hexahydro-1H-4,7-methanoinden-5-yl propionate and 3a,4,5,6,7,7a-hexahydro-1H-4,7-methanoinden-6-yl propionate
- Test material form:
- liquid
1
Method
- Target gene:
- His-gene and Trp-Gene
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97a, TA98, TA100, TA102, TA1535
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- Study 1
Without metabolic activation:
- TA 97a, TA 98, TA 100, TA 1535, TA 102: 0.0016 - 0.005 - 0.016 - 0.05 - 0.16 mg/plate
With metabolic activation:
- TA 100, TA 1535: 0.0016 - 0.005 - 0.016 - 0.05 - 0.16 mg/plate
- TA 97a: 0.005 - 0.016 - 0.05 - 0.16 - 0.5 - 1.6 mg/plate
- TA 98, TA 102: 0.016 - 0.05 - 0.16 - 0.5 - 1.6 mg/plate
Study 2:
Without metabolic activation:
- TA 97a, TA 1535: 0.0016 - 0.005 - 0.016 - 0.05 - 0.16
- TA 98, TA 100, TA 102: 0.005 - 0.016 - 0.05 - 0.16 - 0.5
With metabolic activation:
- TA 97a: 0.0016 - 0.005 - 0.016 - 0.05 - 0.16
- TA 100, TA 1535: 0.005 - 0.016 - 0.05 - 0.16 - 0.5
- TA 98, TA 102: 0.016 - 0.05 - 0.16 - 0.5 - 1.6 - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: dimethylsulfoxide (DMSO)
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- cumene hydroperoxide
- other: ICR 191, 4-Nitro-1,2-phenylenediamine, Nitrofurantoine, 2-Aminoanthraceen, Danthron
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 hours (at 37C)
SELECTION AGENT (mutation assays): agar containing Histidine or Tryptophan
NUMBER OF REPLICATIONS: Three replicates per concentration. Titer control replicates in 2-fold.
DETERMINATION OF CYTOTOXICITY: reduced background lawn was regarded to be a cytotoxic effect. Plates with reduced background lawn were not included into evaluation procedures.
VALIDITY CRITERIA: the following genotypes of the tested strains had to be confirmed:
- Histidine auxotrophy
- Ampicillin resistance
- Tetracycline resistance (only TA 102)
- UV-sensitivity (except TA 102)
- Growth inhibition with crytal violet
Titer of the overnight culture >= 1x10^8 cells/ml.
Spontaneous revertant (negative controls) had to be within the following ranges:
- TA 97a: 60 - 300
- TA 87: 15 - 50
- TA 100: 60 -200
- TA 102: 300 -600
- TA 1535: 5 - 30 - Evaluation criteria:
- Plates with reduced background lawn were not included into evaluation procedures. The induction rate of mean valiues was calculates as revertant colonies of test item/revertant colonies of the corresponding control. The test item is to be interpretated mutagenic if there is a concentration effect relationship and the induction rate is >= 2.
- Statistics:
- Arithmetic mean values and standard deviations were calculated from colonies per plate of three replicates.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 97a
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY: Cytotoxicity was observed in the highest concentrations of all dose levels, and therefore these concentrations were not included in the evaluation process.
Applicant's summary and conclusion
- Conclusions:
- In a bacterial reverse mutation assay (Ames test), cyclaprop did not show mutagenic properties, under the conditions of this test.
- Executive summary:
The mutagenic effects of the test item Cyclaprop were determined in a bacterial reverse mutation assay according to OECD guideline 471 in two independent studies. Test systems were the Salomonella Typhimurium strains TA 97a, TA 98, TA 100, TA 102 and TA 1535 with and without the metabolic actication system S9 (from male Wistar rats) each. Positive and negative controls were included in each study. The duration of each study was 48 hours. The test item was dissolved in DMSO and applied once at test initiation with concentrations ranging from 0.0016 mg/plate - 1.6 mg/plate. The validity criteria of the test were fulfilled.
Cyclaprop did not induce any mutagenicity. Cytotoxicity occured at the highest concentrations tested, therefore these concentrations were not included in the evaluation. Cyclaprop was found to have no mutagenic effects on Salmonella Typhimurium strains with and without S9, under the conditions of this test. According to the criteria outlined in Annex VI of 67/548/EEC (DSD) and Annex I of 1272/2008/EEC (CLP), Cyclaprop does not have to be classified as mutagenic.
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