Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 258-004-5 | CAS number: 52556-42-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15.-24.5.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: sludge taken from activation basin of the STP D-67435 NW-Lachen-Speyerdorf
- Method of cultivation:
- Preparation of inoculum for exposure:
- Pretreatment: filtration, washing with tap water followed by resuspension and aeration until usage in the test
- Initial biomass concentration: sludge: 3.18 g/L dry weight, test: 1.59 g/L dry weight - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 1.08 mmol/L
- Test temperature:
- 21.3-22.5 °C
- pH:
- 8.18
- Nominal and measured concentrations:
- nominal: 3820, 382, 38.2, 3.82 mg/L, respectively 1452, 145, 14.5, 1.45 mg a.i./L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 452 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 452 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 452 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- EC50 (3h) = 8.2 mg/L (95 c.i. > 12 mg/L)
The value lies within the recommendated range for 3,5-dichlorophenol. - Reported statistics and error estimates:
- t-test
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC under test cocentration is 1452 mg a.i./L.
- Executive summary:
In the conducted test, the test item was tested using five concentrations (ranging from 3820 to 3.82 mg/L test item resp. 1452 to 1.45 mg act. ingr./L HAPS). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 3.18 g suspended solids/L, giving a concentration of 1.59 g suspended solids/L in the test.
3,5 -Dichlorophenol was used as positive control. Five concentrations were tested; a 3h-EC50 of 8.2 mg/L (95% c.i.: 3h-EC50 < 12 mg/L) was determined, which lies within the demanded range of 2 -25 mg/L.
As inhibition was below 10 % in the highest treatment (3820 mg/L, using five replicates) and no statistically significant difference to the control was observed, this concentration was stated as the NOEC and no second experiment was performed.
Reference
Table 1:content of test vessels
Number |
Content |
Nominal concentration (mg/L) |
Nutrient Solution (mL) |
H2O (mL) |
Stock solution added (mL) |
Concentration of stock solution (mg/L) |
Inoculum (mL) |
1 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
2 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
3 |
positive control |
5 |
16 |
229 |
5 |
500 |
250 |
4 |
positive control |
10 |
16 |
224 |
10 |
500 |
250 |
5 |
positive control |
20 |
16 |
214 |
20 |
500 |
250 |
6 |
positive control |
40 |
16 |
194 |
40 |
500 |
250 |
7 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
8 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
9 |
test item |
3820 |
16 |
134 |
100 |
19100 |
250 |
10 |
test item |
3820 |
16 |
134 |
100 |
19100 |
250 |
11 |
test item |
3820 |
16 |
134 |
100 |
19100 |
250 |
12 |
test item |
3820 |
16 |
134 |
100 |
19100 |
250 |
13 |
test item |
3820 |
16 |
134 |
100 |
19100 |
250 |
14 |
test item |
382 |
16 |
224 |
10 |
19100 |
250 |
15 |
test item |
38.2 |
16 |
233 |
1 |
19100 |
250 |
16 |
test item |
3.82 |
16 |
234 |
0.1 |
19100 |
250 |
17 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
18 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
19-22 |
* |
* |
* |
* |
* |
* |
* |
23 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
24 |
control |
0 |
16 |
234 |
0 |
-- |
250 |
* other test items were measured in vessels 19-22
Table 2:O2-consumption, inhibition pre-test
Vessel |
Content |
Nominal concentration (mg/L) |
O2consumption (mg/(L x min) |
O2consumption (mg/(L x h) |
Inhibition (%) |
pH |
1 |
control |
0 |
0.5922 |
35.529 |
0 |
8.0 |
2 |
control |
0 |
0.5886 |
35.315 |
0 |
8.0 |
3 |
positive control |
5 |
0.4213 |
25.276 |
32.5 |
8.1 |
4 |
positive control |
10 |
0.2649 |
15.895 |
57.5 |
8.1 |
5 |
positive control |
20 |
0.1285 |
7.711 |
79.4 |
8.2 |
6 |
positive control |
40 |
0.0815 |
4.892 |
86.9 |
8.2 |
7 |
control |
0 |
0.6029 |
36.175 |
0 |
8.0 |
8 |
control |
0 |
0.6167 |
37.001 |
0 |
8.0 |
9 |
test item |
3820 |
0.5953 |
35.721 |
4.6 |
8.3 |
10 |
test item |
3820 |
0.5864 |
35.182 |
6.0 |
8.3 |
11 |
test item |
3820 |
0.6011 |
36.063 |
3.7 |
8.3 |
12 |
test item |
3820 |
0.6098 |
36.588 |
2.3 |
8.2 |
13 |
test item |
3820 |
0.6121 |
36.723 |
1.9 |
8.2 |
14 |
test item |
382 |
0.6357 |
38.143 |
-1.9 |
8.0 |
15 |
test item |
38.2 |
0.6196 |
37.175 |
0.7 |
8.0 |
16 |
test item |
3.82 |
0.6713 |
40.279 |
-7.6 |
7.8 |
17 |
control |
0 |
0.6513 |
39.078 |
0 |
7.9 |
18 |
control |
0 |
0.6566 |
39.397 |
0 |
7.8 |
19-22 |
* |
* |
* |
* |
* |
* |
23 |
control |
0 |
0.6298 |
37.787 |
0 |
8.0 |
24 |
control |
0 |
0.6541 |
39.244 |
0 |
7.9 |
* other test items were measured in vessels 19-22
Description of key information
No significant inhibtion was observed at highest test concentration. Thus, NOEC is stated on the concentration of the active ingredient HAPS.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 452 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.