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EC number: 216-913-4 | CAS number: 1696-20-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In the study BASF 2003, Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 as well as E. coli WPA were exposed to the test item in the presence and absence of a mammalian metabolic activation system (S9-mix) according to the Ames test (1975). Apart from a very slight decrease of the number of revertants that was occasionally observed, results were unanimously indicating the validity of the assay. In this assay, the test item was not mutagenic.
In a mammalian cell micronucleus assay V79 cell cultures were exposed to Acetylmorpholin at concentrations of 0, 40.6, 81.3, 162.5, 325, 650 or 1300 µg/mL (with and without metabolic activation, exp. I), with concentrations of 0, 81.3, 162.5, 325, 650 or 1300 µg/mL (without metabolic activation, exp. II) and 250.0, 500.0, 1000.0 or 1300.0 µg/mL (with metabolic activation, exp. II) and 0, 162.5, 325, 650 or 1300 µg/mL (without metabolic activation, exp. III). Acetylmorpholin was tested up to the limit concentration of 1300 µg/mL (approx. 10 mM). There was no evidence of an induction of micronuclei over background. This study is classified as acceptable. This study satisfies the requirement for Test Guideline In vitro Mammalian Cell Micronucelus Test, OECD 487.
In a mammalian cell gene mutation assay (HPRT), CHO cells cultured in vitro were exposed to Acetylmorpholin at concentrations of 0; 162.5, 325.0, 650.0, 1300.0 µg/mL (1st experiment) in the presence and absence of phenobarbital and β-naphthoflavone-induced rat liver S9 mix and in a 2nd experiment at concentrations of 0; 162.5, 325.0, 650.0, 1300.0 µg/mL (without metabolic activation) and 0; 400.0, 800.0, 1000.0, 1300.0 µg/mL (with metabolic activation). Acetylmorpholin was tested up to limit concentrations of 1300 µg/mL (approx. 10m M). The positive controls did induce the appropriate response. There was no evidence or a concentration related positive response of induced mutant colonies over background. This study is classified as acceptable. This study satisfies the requirement for Test Guideline OPPTS 870.5300, OECD 476 for in vitro mutagenicity (mammalian forward gene mutation) data.
Justification for selection of genetic toxicity endpoint
No stduy was selected because all tests were negative.
Short description of key information:
The test item was not mutagenic in vitro in the Ames test with and without metabolic activation.
The test item was not clastogenic/aneugenic in the in vitro Micronucleus test in V79 cells with and without metabolic activation.
The test item was not mutagenic in vitro in the HPRT test in CHO cells with and without metabolic activation.
Results from a Mouse Lymphoma Test on the substance Ethylmorpholin (CAS Nr.: 100-74-3), used here as a read across study, were negative with and without metabolic activation.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Considering the negative results observed in all genotoxicity tests, Acetylmorpholine is not subject to classification for mutagenicity according to Directive 67/548/EEC and Regulation 1272/2008/EC.
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