4-methoxybenzyl alcohol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: estimated by calculation

Justification for type of information:

estimated by calculation

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT).  It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA).  It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured.  Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow).  Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

341.205 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

not specified

Conclusions:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 341.205 mg/L.

Executive summary:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 341.205 mg/l Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material do not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and cannot be classified as per CLP criteria .

Description of key information

Short-term toxicity to aquatic invertebrates:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 114.788 mg/l Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material do not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and cannot be classified as per CLP criteria .

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

114.788 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

The effect of test material on aquatic invertebrate was evaluaed based on the predicted data of target chemical along with data for structurally and functionally similar read across substance.

In the predicted study the Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 114.788 mg/l Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material do not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and cannot be classified as per CLP criteria .

The above study was supported by structurally similar read across substance ,determination of the inhibition of the mobility of Daphnids was carried out with the test substanceaccording to OECD Guideline 202.

The stock solution 150.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prpared by mixing the stock solution of the test sample in reconstituted water.The test substance was tested at the concentrations 0, 30.0, 45.0, 67.5, 100.0 and 150.0 mg/L.The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated using non linear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 100.8 mg/L for immobilisation effects with 95% CI of 94.7 to 107.4 mg/L. Thus, based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the test substance,does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

The above data was supported by another structurally similar read across substance, short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs. The young daphnids were obtained from continuous cultures in 1 liter glass beakers at 21 ± 0.3 degree C, in dechlorinated and conditioned tap water, the medium was renewed in three weeks and daphnids were fed daily with the green alga Selenastrum capricornutum. The pH of test solution was adjusted to 7.45 ± 0.05 with HCL and NaOH. The concentrations of the resultant solutions were checked before and after (48 h) exposure using a UV–visible spectrophotometer. At the start of the experiment neonate were placed in 50 ml of beaker with 40 ml of test solution. All experiment was made in four replicates and performed at 21 ± 0.3 degree C under 16 h light: 8 h dark photoperiod. Immobility was observed after 24 and 48 hrs and EC 50 value was calculated. After the experiment the EC 50 value of test material for Daphnia magna was determined to be 943 mg/l in 48 hrs of exposure. Based on the CLP regulation the test material is not toxic to aquatic invertebrates and can be considered to be not classified.

The above read across was further supported by data for functionally similar read across substance, short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs. The young daphnids were obtained from continuous cultures in 1 liter glass beakers at 21 ± 0.3 degree C, in dechlorinated and conditioned tap water, the medium was renewed in three weeks and daphnids were fed daily with the green alga Selenastrum capricornutum. The pH of test solution was adjusted to 7.45 ± 0.05 with HCL and NaOH. The concentrations of the resultant solutions were checked before and after (48 h) exposure using a UV–visible spectrophotometer.

At the start of the experiment neonate were placed in 50 ml of beaker with 40 ml of test solution. All experiment was made in four replicates and performed at 21 ± 0.3 degree C under 16 h light: 8 h dark photoperiod. Immobility was observed after 24 and 48 hrs and EC 50 value was calculated. The EC 50 value of test material for Daphnia magna was determined to be 908 mg/l in 48 hrs.

Based on the CLP regulation the test material is not toxic to aquatic invertebrates and can be considered to be not classified.