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EC number: 428-100-3 | CAS number: 94239-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance ID: F6TF
Lot #: AF0460
Purity: 99.5% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: high performance liquid chromatography method; As undissolved material was observed in the test vessels all samples were analysed both pre and post-centrifugation. At the start of the test, samples were taken of each excess test solution, immediately after filling the test vessels. At the end of the test, each blank solution was sampled and analysed in the same manner, immediately on opening. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances): This study was run with a culture medium control and a solvent control together with nominal test substance concentrations of 2.2,4.4, 8.8, 18, 36 and 72 mg/L. The test substance was volatile and was not readily dissolved in water, therefore stock solutions in dimethylformamide were prepared. Each test solution was prepared, in completely full, glass stoppered volumetric flasks, to minimise volatilisation, by the addition of an aliquot of the relevant stock solution to sterile culture medium. These introductions were made, after equalising additions of dimethylformamide, where applicable, such that the solvent control and all test concentrations contained 0.10 mL/L of dimethylformamide. The control solution, similarly treated, consisted of culture medium only. All additions were made sub-surface and gradually by microlitre syringe, into solutions gently stirred by magnetic follower. The stirring duration was minimised due to the volatile nature of the test substance. After preparation, a visual assessment of the test solutions showed both controls and the nominal 2.2 mg/L test concentration to be clear and colourless. All higher nominal test concentrations contained increasing densities of white particles in homogeneous stirred suspension. Some small, colourless globules were also seen in the 18, 36 and 72 mg/L test solutions. However subsequent analysis showed that the test solution concentrations were within the required range. Using aseptic techniques, 60 mL volumes of the appropriate test solution were dispensed to each test and blank vessel, with the remainder of the test solutions being used for physical and chemical analyses.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: unicellular green alga Selenastrum capricornutum
- Strain: Strain ATCC 22662
- Source (laboratory, culture collection): ATCC
- Age of inoculum (at test initiation): A 3 day old culture of the alga in the exponential growth phase was used as inoculum for the test.
- Method of cultivation: The culture was grown in the medium, and under the environmental conditions, described for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 ± 2°C
- pH:
- Test start: 8.6
Test end: 8.8 - 9.3 - Nominal and measured concentrations:
- Nominal concentrations: 2.2, 4.4, 8.8, 18, 36 and 72 mg/L
Measured concentrations: starting post-centrifugation 2.3, 4.5, 8.6, 17, 34 and 64 mg/L
Starting post-centrifugation measured concentrations ranged from 89 to 105% of nominal.
The study end post-centrifugation measured concentrations in the blanks ranged from 88 to 98% of nominal values. - Details on test conditions:
- - Test vessel: borosilicate glass bottles of 50 mL nominal capacity filled completely (approximately 60 mL of test solution) with airtight, PTFE septa and aluminium caps.
- Temperature: incubated at 24 ± 2ºC with orbital shaking
- No. of vessels per concentration (replicates): 2 test substance, 2 solvent control, 4 culture medium control
- Test concentrations: culture medium control, solvent control, 2.2, 4.4, 8.8, 18, 36 and 72 mg/L test substance
- Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: After preparation, a visual assessment of the test solutions showed both controls and the nominal 2.2 mg/L test concentration to be clear and colourless. All higher nominal test concentrations contained increasing densities of white particles in homogeneous stirred suspension. Some small, colourless globules were also seen in the 18, 36 and 72 mg/L test solutions. However subsequent analysis showed that the test solution concentrations were within the required range. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- > 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Reported statistics and error estimates:
- The area under the growth curve (days 0 to 3) was calculated for each replicate culture, according to the formula given in the OECD Guideline. These areas were examined by one-way analysis of variance. Dunnett's procedure was used to identify significant differences (P=0.05) from the solvent control.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 72-hour EbC50 > 72 mg/L
72-hour ErC50 > 72 mg/L - Executive summary:
The toxicity of the test substance to the green algae, Selenastrum capricornutum, was determined in a 72-hour, static toxicity test. Test vessels were filled to the top and sealed to prevent loss of the test substance. The vessels were incubated at approximately 24°C with orbital shaking. The following endpoints were calculated:
72-hour ErC50 > 72 mg/L
72-hour EbC50 > 72 mg/L
Reference
Description of key information
Based on nominal concentrations because mean measured concentrations were within 80-120% of nominal:
72-hour ErC50 > 72 mg/L
72-hour EbC50 > 72 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 72 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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