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EC number: 216-378-7 | CAS number: 1569-69-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional physico-chemical information
Administrative data
- Endpoint:
- other: Analytical validation method
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2012-10-10 to 2012-11-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in accordance with an acceptable scientific method and in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Sanco 3029
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclohexanethiol
- EC Number:
- 216-378-7
- EC Name:
- Cyclohexanethiol
- Cas Number:
- 1569-69-3
- Molecular formula:
- C6H12S
- IUPAC Name:
- cyclohexanethiol
- Reference substance name:
- Cyclohexylmercaptan
- IUPAC Name:
- Cyclohexylmercaptan
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): Cyclohexylmercaptan
- Substance type: monoconstituent substance
- Physical state: clear colourless liquid
- Analytical purity: 99.97%
- Purity test date: 2012-05-31
- Lot/batch No.: 2012-05-1765
- Expiration date of the lot/batch: 2014-06-07
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark under nitrogen
Constituent 1
Constituent 2
Results and discussion
- Results:
- The analytical method is succesfully validated
Any other information on results incl. tables
Specificity
Chromatograms of a test substance solution and the blank accuracy sample are shown in Figure 1and Figure 2 (attached), respectively.
The chromatogram of the blank accuracy sample showed no peak at the retention time of the test substance. Since no interferences were detected, the specificity requirements were met and the analytical method was found to be specific for the test substance.
Linearity
The calibration line was constructed using all data points. Figure 3 (attached) illustrates the calibration curve and Table 1 shows the statistical parameters.There was a linear relationship between response and test substance concentration in the range of 3.98 – 598 mg/l (in end solution). Since the coefficient of correlation (r) was > 0.99 and the back calculated accuracies of the data points were in the range 85-115% the calibration line was accepted.
Table 1 Statistical parameters of the calibration curve
|
|
Slope |
1.61´103 |
Intercept |
2.75´102 |
Weighting factor |
1/concentration2 |
r |
0.9998 |
|
|
Accuracy and repeatability
The results of the accuracy samples are given in Table 2. Since the mean accuracy at each concentration level fell in the criterion 70-110% and the coefficient of variation was ≤ 20% the analytical method was accepted for the analysis of the test substance in water in the target concentration range of 10 - 1000 mg/l.
Table 2 Accuracy samples
Target concentration |
Nominal concentration |
Analysed concentration |
Accuracy |
Mean accuracy |
Coefficient of variation |
|
|
|
|
|
|
10 |
9.97 |
9.48 |
95 |
98 |
7.3 |
|
9.97 |
9.90 |
99 |
|
|
|
9.97 |
9.29 |
93 |
|
|
|
9.97 |
11.0 |
110 |
|
|
|
9.97 |
9.32 |
94 |
|
|
|
|
|
|
|
|
1000 |
997 |
987 |
99 |
100 |
0.55 |
|
997 |
1002 |
100 |
|
|
|
997 |
992 |
100 |
|
|
|
997 |
996 |
100 |
|
|
|
997 |
992 |
100 |
|
|
|
|
|
|
|
|
Limit of quantification
The limit of quantification (LOQ) was assessed as 10 mg/l in water.
Stability of analytical system and end solutions
The results of the stability of the analytical system and end solutions are given in Table 3. Since the coefficient of variation at both concentration levels was ≤ 20% the analytical system and end solutions were stable over at least a 3.65 hour time interval.
Table 3 Stability of the analytical system and end solutions
Nominal concentration |
Elapsed time |
Coefficient of variation |
|
|
|
3.98 |
4.50 |
2.5 (n=3) |
|
|
|
15.0 |
4.36 |
2.0 (n=3) |
|
|
|
598 |
3.65 |
1.3 (n=3) |
|
|
|
Stability of stock solutions
The coefficient of variation on the response factors of the calibration solutions prepared with fresh and stored stock solutions was 0.31%. Since the value was ≤ 10% the stock solutions were stable when stored at room temperature for at least 1 day.
Conclusion
The analytical method was validated for the following parameters:
Specificity |
specific |
Linearity |
r = 0.9998 |
Accuracy |
98 and 100% |
Repeatability |
7.3 and 0.55% |
Limit of quantification |
10 mg/l |
Stability analytical system and end solutions |
Stable (3.65 hours) |
Stability stock solutions |
Stable (1 day) |
Applicant's summary and conclusion
- Conclusions:
- The validation of the analytical method was succesfully performed.
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