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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed study, but without GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetoacetamide
EC Number:
227-774-4
EC Name:
Acetoacetamide
Cas Number:
5977-14-0
Molecular formula:
C4H7NO2
IUPAC Name:
acetoacetamide

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Origin: Pseudomonas putida, DSM 50026 by Deutsche Sammlung Mikroorganismen, Mascheroder Weg, 1d, D-38124 Braunschweig
Pre culture and test batches: according to DIN standard
State of acclimatization: not acclimatized

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
16.5 h
Post exposure observation period:
none

Test conditions

Test temperature:
21 - 22 °C
Details on test conditions:
To keep the bacteria in suspension, the test batches were conducted in an icubation rotary shaker with a diameter of the shaking circle of 5 cm. The shaking frequency was set to about b220 rpm. The tested batches were incubated for a period of 16.5 hours. The temperature was controlled at 21 - 22 °C according to the DIN standard method cited. The composition of reaction medium corresponds to the DIN standard cited. The concentration of the growth subtrate glucose was 2000 mg/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
16.5 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16.5 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The results of testing the toxicity of acetoactamid (approx. 30% solution) on the growth of bacteria Pseudomonas putida cell multiplication inhibition test revealed that the EC 10 and EC50 values of acetoacetamid (approx. 30% solution in water are > 1000 mg/L. The corresponding EC10 and EC50 values of the pure substance acetoacetamide are > 297 mg/L