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EC number: 939-039-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-07-20 to 2017-09-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- No analytical determination of the test item was performed. According to the information of the sponsor, the test item is insoluble in water. Therefore, the water accommodated fraction (WAF) was the method of preparation of the test solutions.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The water-accommodated fraction was prepared for the test. This was done by weighing the nominal load 100 mg/L, (real load 100.0 mg/L) adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm Cellulose ester filters.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species Danio rerio HAMILTON-BUCHANAN
Age sexually immature young fish, length 2 +- 1 cm
The animals were obtained from a commercial supplier. Before being used for the test, the fish were kept for at least 12 days under test conditions. During 7 days, mortality didn’t surpass 5 %.
Supplier: Dehner Garten Center, 67433 Neustadt
Date of arrival: 03. May 2017 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 0h: 23.6 °C
24h: 23.4 °C
48h: 22.9 °C
96h: 23.1 °C - pH:
- Control: 8.1 (0h), 8.1 (24h), 8.1 (48h), 8.1 (72h), 8.0 (96h)
Test item: 8.1 (0h), 8.1 (24h), 8.1 (48h), 8.1 (72h), 8.1 (96h) - Dissolved oxygen:
- Control: 8.8 mg/L (0h), 8.2 (24h), 8.1 (48h), 8.3 (72h), 8.1 (96h)
Test item: 8.6 mg/L (0h), 8.2 (24h), 8.1 (48h), 8.3 (72h), 8.1 (96h) - Nominal and measured concentrations:
- Nominal: 0 (Control), 100 mg/L
- Details on test conditions:
- 24 hours before the start of the test, the test fish were no longer fed.
Date: 31. Jul. – 04. Aug. 2017
Treatments 100 mg/L
Test Design static
Medium renewal none
Duration 96 hours
Loading 1 fish/L
Vessels: glass aquaria, maximal volume 10 L
Aeration: accomplished with glass tubes
Feeding: none
Photo period: 12/12 hours using neon tubes
Temperature: 22.9 – 23.6 °C (deviation within on test +- 1 °C)
pH adjustment: none
Replicate (Treatments): 1 vessel, each containing 7 L test solution and 7 fish
Replicate (Blank Control): 1 vessel, containing 7 L tap water and 7 fish
Observations were made every 24 hours, measuring pH and O2-concentration of the test solution in each vessel and documenting mortalities or abnormal behaviour.
Vessels polyethylene aquaria
Medium chlorine-free tap water
Feeding three times a day with warmwater fish food, totalling about 1-2% of body weight per day
Photo period 12/12 hours, using neon tubes
Temperature 23 +- 2 °C - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No effects up to the limit of solubility
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No effects up to the limit of solubility
- Sublethal observations / clinical signs:
Mortality
The mortalities were recorded daily and can be found in the following table.
Nominal Concentration in mg/L
Total number of fish used
Number of dead fish
24 h
48 h
72 h
96 h
Blank control
7
0
0
0
0
100
7
0
0
0
0
No abnormal behaviour of the surviving fish was observed.
Results
The biological results of the test item are presented in the following table:
Parameter
Value
NOEC
=100 mg/L
LOEC
> 100 mg/L
96h LC50
> 100 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity to Danio rerio was tested using a static test design.
The study was performed as a limit test at the nominal concentration 100 mg/L. Neither in the treatment nor in the control could any mortality be observed.
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
The result of the test is considered valid. - Executive summary:
The toxicity to Danio rerio was tested using a static test design.
The study was performed using 1 concentration containing 100 mg/L nominal concentration. The concentration showed no significant mortality.
The following results for the test item could be determined:96h-NOEC = 100 mg/L
96h-LOEC > 100 mg/L
96h-EC50 > 100 mg/LThe study met the validity criteria specified in the test guideline, as follows:
¨ The mortality in the blank control may not exceed 1 fish at the end of the test.
No mortality occurred in the blank control.¨ The dissolved oxygen concentration must be at least 60% throughout the test.
The concentration of dissolved oxygen was at least 8.1 mg/L or 95 % throughout the test.¨ The pH-value in the test solutions should not vary by more than 1 unit during the test.
The highest variation was 0.1 unit.
Reference
Description of key information
The acute toxicity of the test item to fish was tested in an experiment performed according to the OECD Test Guideline 203 and to GLP requirements.
The 96 LL50 was determined to be above the only concentration tested (100 mg/L) - no effects were observed up to the limit of solubility.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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