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EC number: 269-505-3 | CAS number: 68259-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 84/449(1984), Part. C9
- Deviations:
- no
- Principles of method if other than guideline:
- A predetermined amount of the test substance dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Details on inoculum:
- None
- Duration of test (contact time):
- 2 h
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Details on study design:
- Sample weight: 5 mg of the test substance
Temperature: 148 ± 3 °C
Duration: 2 h
Estimation Technique: Titration (Potentiometric), METROHM Titroprozessor 670 (SAV : 00 111) - Reference substance:
- other: Potassium hydrogen phthalate solution
- Preliminary study:
- No data
- Test performance:
- No data
- Remarks on result:
- not measured/tested
- Details on results:
- No data
- Key result
- Parameter:
- COD
- Value:
- 507 mg O2/g test mat.
- Key result
- Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Results with reference substance:
- The COD of the reference substance is 204 mg O2/L (Criteria: 200±8 mg O2/L)
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The test substance showed no biodegradation potential.
- Executive summary:
The chemical oxygen demand (COD) of the test substance was evaluated in this study.
A predetermined amount of the test substance (of 100 % purity) dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate (according to DEV H 41-1, DEV 1980).
The substance was tested at 5 mg/L.
Under the study conditions, the COD of the test substance was 507 mg O2/g. Further, the BOD5 value from another experiment was 0 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.
- Endpoint:
- biodegradation in water: ready biodegradability
- Remarks:
- BOD5
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 27 January, 1993 to 19 February, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
- Deviations:
- yes
- Remarks:
- (aerate overnight after addition of the inoculum)
- Principles of method if other than guideline:
- No data
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Seeding water of an urban waste water
- Details on inoculum:
- - Source of inoculum/activated sludge: Seeding water of an urban waste water
- Duration of test (contact time):
- 5 d
- Initial conc.:
- 6.4 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12.8 mg/L
- Based on:
- test mat.
- Initial conc.:
- 25.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 51.4 mg/L
- Based on:
- test mat.
- Initial conc.:
- 102.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 205.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 411 mg/L
- Based on:
- test mat.
- Initial conc.:
- 821.9 mg/L
- Based on:
- test mat.
- Details on study design:
- Temperature: 20 ± 1 °C
Duration: 5 d
Estimation Technique: O2 determination, measured with an oxygen sensitive electrode system: SYLAND (SAV: 00 107)
Suppression of nitrification: without suppression of nitrification - Reference substance:
- other: D(+)-Glucose /L-Glutamic acid
- Preliminary study:
- No data
- Test performance:
- No data
- Remarks on result:
- not measured/tested
- Details on results:
- The BOD5 of the test substance corrected by the blank control is 0 mg O2/g. Further, the COD value was taken from an another experiment and was found to be 507 mg O2/L. The % biodegradation was calculated using the below formula: BOD/COD*100.
Hence, the biodegradation of the test substance is 0 %. - Key result
- Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Key result
- Parameter:
- COD
- Value:
- 507 mg O2/g test mat.
- Remarks on result:
- other: from a different study
- Results with reference substance:
- The BOD5 of glucose/glutamic acid solution is 197 mg O2/L.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance showed no biodegradation potential.
- Executive summary:
The biochemical oxygen demand of the test substance (of ca. 100 % purity) was determined by measuring the dissolved oxygen concentration before and after 5 d incubation at 20 °C with different test concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test was carried out according to ISO 5815 in compliance with GLP.
The substance was tested at concentrations from about 6 to 800 mg/L.
Under the study conditions, the BOD5 of the test substance corrected by the blank control was 0 mg O2/g. Further, the COD value from another experiment was found to be 507 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 13 January, 1993 to 25 March, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Method 8192 B
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Sludge of a biological sewage treatment plant (ARA-Basel lndustrie)
- Amount of sludge: 910 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 150 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Water/nutrient: According to the Guideline
Temperature: 22±3 °C (room temperature)
Test duration: 28 d
Lighting: Indirect daylight
Estimation technique: TOC/DOC analyzer / SHIMADZU TOC-500 - Reference substance:
- diethylene glycol
- Preliminary study:
- The mean value of a preliminary test was 154.5 mg/L.
- Test performance:
- No data
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 3.1
- Sampling time:
- 28 d
- Details on results:
- The bioelimination of the test substance measured as DOC after 28 d corrected by the blank control was 0 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 0 %.
- Results with reference substance:
- The bioelimination, corrected by the blank control and measured as DOC (mg/L) was 99.1 % on the 23rd day.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The test substance is not inherently biodegradable.
- Executive summary:
The inherent biodegradability of the test substance (of ca. 100 % purity) was determined in a 28 d Zahn-Wellens test according to OECD Guideline 302B and ISO Method 8192 B in compliance with GLP.
The substance was tested at a concentration of 150 mg/L DOC.
The bioelimination measured as DOC corrected by the blank control was 0 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 0 %.
Based on these findings, the test substance is not considered to be inherently biodegradable.
Referenceopen allclose all
COD analytical data:
Product |
Sample weight E (mg) |
Titration (mL) |
N= |
mg O2/g |
||
|
|
a |
b |
c |
|
|
Normality |
|
|
|
10.108 10.116 |
0.1187 0.1186 Ǿ : 0.1186 |
|
Blank |
|
9.818 9.804 Ǿ : 9.811 |
|
|
|
|
Test substance |
5 mg 5 mg |
|
7.146 7.130 |
|
|
506 509 Ǿ :507 |
BOD values of the oxygen measurements:
Substance |
Test concentration E = mg/L |
Start mg O2/L T0 |
End mg O2/L T5 |
Blank mg O2/L B1 |
mg O2/g BOD5 |
Test substance |
821.9 |
8.7 8.6 |
7.3 8.0 |
0.7 0.7 |
1 0 |
411.0 |
8.7 8.6 |
8.0 7.9 |
0.7 0.7 |
0 0 |
|
205.5 |
8.7 8.6 |
8.0 8.0 |
0.7 0.7 |
0 0 |
|
102.7 |
8.7 8.6 |
8.2 8.0 |
0.7 0.7 |
0 0 |
|
51.4 |
8.7 8.6 |
7.9 8.1 |
0.7 0.7 |
2 0 |
|
25.7 |
8.7 8.6 |
8.0 8.2 |
0.7 0.7 |
0 0 |
|
12.8 |
8.7 8.6 |
8.1 7.8 |
0.7 0.7 |
0 8 |
|
6.4 |
8.7 8.6 |
8.1 8.1 |
0.7 0.7 |
0 0 |
Table:
Time |
Reference |
Test substance |
||||
Mean value of the reference DOC (mg/L) |
Value of the blank DOC (mg/L) |
Elimination % |
Mean value of the substance DOC (mg/L) |
Value of the blank DOC (mg/L) |
Elimination % |
|
-1 d |
153.5 |
- |
- |
154.5 |
- |
- |
0 |
169.8 |
19.6 |
2.2 |
172.5 |
19.6 |
1.0 |
3 h |
170.1 |
14.3 |
0 |
175.7 |
175.7 |
0 |
2 d |
169.1 |
13.4 |
0 |
179.2 |
13.4 |
0 |
5 d |
175.0 |
21.7 |
0.1 |
194.6 |
21.7 |
0 |
7 d |
165.6 |
20.4 |
5.4 |
191.6 |
20.4 |
0 |
9 d |
153.3 |
18.7 |
12.3 |
187.4 |
18.7 |
0 |
12 d |
153.3 |
18.7 |
12.3 |
187.4 |
18.7 |
0 |
13 d |
147.4 |
19.8 |
16.9 |
190.1 |
19.8 |
0 |
14 d |
67.1 |
14.7 |
65.9 |
180.8 |
14.7 |
0 |
15 d |
19.0 |
12.4 |
95.7 |
178.8 |
12.4 |
0 |
16 d |
17.4 |
11.7 |
96.3 |
- |
- |
- |
19 d |
13.7 |
11.6 |
98.6 |
182.4 |
11.6 |
0 |
21 d |
12.8 |
9.3 |
97.7 |
- |
- |
- |
23 d |
11.9 |
10.5 |
99.1 |
170.8 |
10.5 |
0 |
26 d |
- | - | - | 179.0 |
12.4 |
0 |
28 d |
- |
- |
- |
171.9 |
9.8 |
0 |
Mean values of the double determination
Description of key information
The biodegradability of Reactive Black 039 was determined based on the BOD5/COD quotient calculated. The test to determine the biological oxygen demand was performed on test concentrations from 6 to 800 mg/L following ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method). In this study biodegradation of test substance was equivalent to 0 mg O2/g. The COD was estimated to be 507 mg O2/g in a separately conducted study. As a result the quotient of BOD5/COD is 0 indicating that the substance is not biodegradable. In addition, an inherent biodegradation test following OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) was conducted with the test item. As a result 3.1% DOC removal were found at the end of of the study period (44 days) supporting the initial finding, that the substance is neither readily nor inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.