3-hydroxy-2'-methoxy-2-naphthanilide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: According to OECD 209, but limited documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-2'-methoxy-2-naphthanilide

EC Number:

205-206-6

EC Name:

3-hydroxy-2'-methoxy-2-naphthanilide

Cas Number:

135-62-6

Molecular formula:

C18H15NO3

IUPAC Name:

3-hydroxy-N-(2-methoxyphenyl)naphthalene-2-carboxamide

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Due to the low solubility no stock solution was used. The test item was weighed directly into the test vessels.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Origin: sewage treatment plant at Frankfurt am Main, Sindlingen (domestic activated sludge)
- Pretreatment: corresponding to the cited test guideline

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Test temperature:

20 ± 2°C

pH:

7.2 ± 0.2

Nominal and measured concentrations:

Nominal concentrations: 500 and 1 000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glasbottles with a diameter of 60 mm and a grounded neck (NS 29), height 170 mm, total volume appr. 480 ml. The bottles contained magnetic stirrer bars and were incubated in a water bath on magnetic stirrers.
- Test volume: 200 ml each batch
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item is not toxic at a concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant.

Executive summary:

A Respiration Inhibition Test according to OECD Guideline No. 209 was performed with the test item at Hoechst AG, Abteilung Umweltschutz, Frankfurt. The respiration rate of test item was measured after a contact time of three hours. The EC50 is > 1000 mg/L to activated sludge.