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EC number: 202-728-6 | CAS number: 99-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-nitrotoluene
- EC Number:
- 202-728-6
- EC Name:
- 3-nitrotoluene
- Cas Number:
- 99-08-1
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 3-nitrotoluene
- Details on test material:
- - Name of test material (as cited in study report): m-Nitrotoluene
- Physical state: liquid (light yellow oil)
- Analytical purity: 99.5%
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hochst AG, Kastengrund, Germany, conventional breeding
- Age at study initiation: about 3-5 months
- Weight at study initiation: 2.0-2.7 kg
- Housing: animals were housed in individual cages in full air-conditioned rooms
- Diet (e.g. ad libitum): Altromin 2123 Haltungsdiät-Kaninchen, Altromin GmbH, Lage/Lippe, Germany, ad libitum plus hey (about 15 g daily)
- Water (e.g. ad libitum): deionize, chlorinated water from automatic drinking trough
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 3°C
- Humidity (%):50 ± 20%
- Photoperiod (hrs dark / hrs light): 12 hours per day
IN-LIFE DATES: From: 04.02.1986 To: 07.02.1986
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin areas serve as control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL per plaster - Duration of treatment / exposure:
- 4 hours
- Observation period:
- 30-60 minutes, 24, 48 and 72 hours
- Number of animals:
- 3
- Details on study design:
- Approximately 24 hours prior to the start of the experiment 3 rabbits were depilated in the dorsal area of the body with an electric hair clipper on an area of about 25 cm². Only animals with intact skin were used.
On each animal was fixed an adhesive plaster with 2.5 x 2.5 cm cellulose layer on the prepared skin site. Under this plaster 0.5 mL of the undiluted substance was applied. Subsequently, the area was covered with a semiocclusive bandage.
The exposure time was 4 hours. After the exposure period, the remaining test substance was carefully removed from the skin with tepid tap water.
The assessments were made for 30-60 minutes and then at 24, 48, and 72 hours after the removal of the patch.
The erythema and eschar formation and edema ware evaluated numerically according to DRAIZE score method.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no erythema was reported
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no erythema was reported
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no erythema was reported
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no edema was reported
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no edema was reported
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: no edema was reported
- Irritant / corrosive response data:
- During the complete test time, no irritation occurred.
Any other information on results incl. tables
Time after the patch Removal |
24 h |
48 h |
72 h |
||||||
Animal |
1 |
2 |
3 |
1 |
2 |
3 |
1 |
2 |
3 |
Erythema andescharformation |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Executive summary:
Weigand (1986):
The skin irritation potential of 3-nitrotoluene was tested according to OECD guideline 404.
Approximately 24 hours prior to the start of the experiment 3 rabbits were depilated in the dorsal area of the body with an electric hair clipper on an area of about 25 cm². Only animals with intact skin were used.
On each animal was fixed an adhesive plaster with 2.5 x 2.5 cm cellulose layer on the prepared skin site. Under this plaster 0.5 mL of the undiluted substance was applied. Subsequently, the area was covered with a semiocclusive bandage.
The exposure time was 4 hours. After the exposure period, the remaining test substance was carefully removed from the skin with tepid tap water.
The assessments were made for 30-60 minutes and then at 24, 48, and 72 hours after the removal of the patch.
The erythema and eschar formation and edema ware evaluated numerically according to DRAIZE score method.
During the complete test time, no irritation occurred.
On the basis of these results 3-nitrotoluene was considered not irritating to the skin.
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