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EC number: 243-039-0 | CAS number: 19406-86-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Toxicity test to microorganisms performed within OECD 301F (Ready Biodegradability Manometric Respirometry Test)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-amino-p-toluamide
- EC Number:
- 243-039-0
- EC Name:
- 3-amino-p-toluamide
- Cas Number:
- 19406-86-1
- Molecular formula:
- C8H10N2O
- IUPAC Name:
- 3-amino-p-toluamide
Constituent 1
Test organisms
- Test organisms (species):
- other: activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the municipal sewage treatment plant, 31137 Hildesheim, Germany
- Preparation of inoculum for exposure: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspeded in min-eral salts medium and was maintained in an aerobic condition by aera-tion for 2 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and main-tained in an aerobic condition by aeration with CO2-free air for 6 days. 10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration: 4.2 x 10e9 CFU/L
- Water filtered: no
Study design
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
Test conditions
- pH:
- 7.75 (start) - 7.96 (end)
- pH adjusted: no - Nominal and measured concentrations:
- Toxicity Control
28mg/L Test Item + 45 mg/L Reference Item (nominal) - Details on test conditions:
- Toxicity test done within OECD301F:
TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301F
- Additional substrate: No
- Test temperature: Nominal: 20 - 24, ± 1 °C; Actual: 21.4 – 21.7 °C
- pH:
pH-Values at Test Start and Test End
pH-Value
Start End
Inoculum Control 7.77 1) 7.73
2) 7.75
Functional Control 7.77 7.99
Test Item 7.74 1) 7.73
2) 7.74
Toxicity Control 7.75 7.96
- pH adjusted: no
- Dispersion treatment: Continuous stirring
- Continuous darkness: Dark, in an incubator
TEST SYSTEM
- Culturing apparatus: Incubator
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Closed bottles with a rubber sleeve with soda lime
- Measuring equipment: The bottles were closed with OxiTOP measuring heads.
The oxygen consumption was determined in the incubation vessels by the OxiTop® measuring system at 360 measuring points (every 112 minutes) during the 28 d incubation period.
SAMPLING
- Sampling frequency: 360 measuring points (every 112 minutes) during the 28 d incubation period.
- Sampling method: Pressure measurements directly from the test vessels
CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and reference item
- Abiotic sterile control: No
- Toxicity control: Test item and reference item in test concentrations
STATISTICAL METHODS:
Calculation of the theoretical oxygen demand (ThOD) of the test item and functional control.
According to OECD-guideline 301, the index of the atoms of the item CcHhClclNnNanaOoPpSs was used for the calculation of the ThOD.
The oxygen depletion of the test and reference item was calculated out of the difference of the test and inoculum control replicates.
The biodegradation of the test and reference item was calculated out of the BOD in relation to the ThOD. - Reference substance (positive control):
- yes
- Remarks:
- benzoic acid, sodium salt
Results and discussion
Any other information on results incl. tables
In the toxicity control the biodegradation achieved 53 % after 14 days. After 28 days the biodegradation came to 56 %.
Replicate | Biodegradation [%] |
|||
Study Day [d ] |
||||
7 | 14 | 21 | 28 | |
Toxicity Control: 28mg/L Test Item + 45 mg/L Reference Item | 48 | 53 | 54 | 56 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The percentage degradation of the toxicity control reached the pass level of 25 % within 2 days (validity criterion: 25 % after 14 days).
- Conclusions:
- In the toxicity control containing both test and reference item 53 % degradation occurred within 14 days. After 28 days the
biodegradation came to 56 %. The degradation of the reference item was not inhibited by the test item.
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