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EC number: 914-172-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
See read-across justification report under Section 13 ‘Assessment Reports’.
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
In accordance with REACH Annex XI, Section 1.5, of Regulation (EC) No. 1907/2006 (REACH) the standard testing regime may be adapted in cases where a grouping or read-across approach has been applied.
The similarities may be based on:
(1) a common functional group
(2) the common precursors and/or the likelihood of common breakdown products via physical or biological processes, which result in structurally similar chemicals; or
(3) a constant pattern in the changing of the potency of the properties across the category
(1) The target substance is a reaction mass of calcium hydrogenorthophosphate and calcium bis(dihydrogenorthophosphate). The source substance of calcium hydrogenorthophosphate is therefore expected to give similar results.
(2) All members of the group will ultimately dissociate into the common breakdown products of the Ca2+ cation and the PO43-anion.
(3) Calcium orthophosphates are expected to dissociate to the ionic forms in water and therefore the toxicity results can be read across on the basis that cations are abundant in natural waters and are unlikely to contribute to toxicity.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See read-across justification report under Section 13 ‘Assessment Reports’.
3. ANALOGUE APPROACH JUSTIFICATION
See read-across justification report under Section 13 ‘Assessment Reports’.
4. DATA MATRIX
See read-across justification report under Section 13 ‘Assessment Reports’. - Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L. Samples were taken after 1, 24, 48, 72, and 96 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Tap water was passed through a membrane filter (1 μm) to remove particulate matter and then a high-grade activated carbon filter to remove any organic contaminants. Preliminary solubility trials indicated that this test substance did not produce test solution of 100 mg/L using organic solvents such as acetone, dimethyl sulfoxide (DMSO), ethanol, and N,N-dimethylformamide (DMF). Therefore, the test solution was prepared by direct addition of the test substance to dilution water. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese rice fish
Source: Korea Institute of Toxicology (KIT), KRICT, Deajeon, Korea
- Age at study initiation (mean and range, SD): 3-4 month
- Length at study initiation (length definition, mean, range and SD): 2.0±1.0 cm
- Weight at study initiation (mean and range, SD): 0.19 g (S.D. = 0.03 g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: same as in test
- Feeding frequency: not fed 24 hours prior to test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 10-250 mg/L CaCO3
- Test temperature:
- 23.3 ± 0.1 °C
- pH:
- 7.18-7.97
- Dissolved oxygen:
- 5.2-8.5 mg/L
- Salinity:
- <0.01 mg/L (residual chlorine)
- Nominal and measured concentrations:
- Nominal test substance concentration: 100 mg/L
The concentrations of the test substance were measured to be 13.0-14.2 mg/L at a nominal concentration of 100 mg/L (13.0-14.2% of nominal concentration) at 0, 48, and 96 hours. - Details on test conditions:
- TEST SYSTEM
- Aeration:none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered tap water (1 µm, membrane filter), residual chlorine or organic substances were removed by filtering through an activated carbon filter.
- Chlorine: < 0.01 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 990-1060 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and abnormality after 1, 24, 48, 72, and 96 hours
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality observed at 100 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 13.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 13.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There were no mortalities and adverse effects observed in any of the fish exposed to control and 13.5 mg/L treatment effect group during the test. All results were expressed in terms of the mean measured concentration.
- Reported statistics and error estimates:
- No statistics performed
- Sublethal observations / clinical signs:
Table 1. Cumulative mortality of Oryzias latipes
Nominal concentration (mg/L)
Mean measured concentration (mg/L)
Number of organisms tested
Cumulative number of dead fish
24-hour
48-hour
72-hour
96-hour
Control
ND
7
0
0
0
0
100
13.5
7
0
0
0
0
* ND : Not detected
Table 2. Symptoms of intoxication of the test substance to Oryzias latipes
Nominal concentration (mg/L)
Mean measured concentration (mg/L)
Symptoms of Intoxication
24-hour
48-hour
72-hour
96-hour
Control
ND
NOR(7)
NOR(7)
NOR(7)
NOR(7)
100
13.5
NOR(7)
NOR(7)
NOR(7)
NOR(7)
* ND : Not detected
* ( ) : Number of fish
* NOR : Normal
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the mean measured concentration a LC50(96 h) > 13.5 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.
- Executive summary:
The acute toxicity of Calcium hydrogenorthophosphate to fish was investigated in a study following OECD guideline 203 (Kim et al. 2013). The test was set up as a limit test under static conditions. Fish of the species Oryzias latipes were exposed to a nominal test substance concentration of 100 mg/L. The analytical recovery rate of the test substance was 13.0-14.2% of the nominal concentration. During the test period of 96 hours mortality and abnormal behavior of the test organisms were examined after 1, 24, 48, 72, and 96 hours. Mortality or adverse effects of the test substance were not observed. Based on the mean measured concentration a LC50(96 h) > 13.5 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.
Reference
Description of key information
Data on the acute toxicity of the substance itself are not available. The assessment was therefore based on studies conducted with the analogue substance calcium hydrogenorthophosphate (7757-93-9) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5.
The acute toxicity of Calcium hydrogenorthophosphate to fish was investigated in a study following OECD guideline 203 (Kim et al. 2013). The test was set up as a limit test under static conditions. Fish of the species Oryzias latipes were exposed to a nominal test substance concentration of 100 mg/L. The analytical recovery rate of the test substance was 13.0-14.2% of the nominal concentration. During the test period of 96 hours mortality and abnormal behavior of the test organisms were examined after 1, 24, 48, 72, and 96 hours. Mortality or adverse effects of the test substance were not observed. Based on the mean measured concentration a LC50(96 h) > 13.5 mg/L (nominal: LC50(96 h) >100 mg/L) was determined.
Comparable results were determined in studies on the toxicity of sodium and potassium phosphates to fish.
Based on the available data the assessed substance calcium bis(dihydrogenorthophosphate) is considered not acutely toxic to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
nominal LC50 >1,000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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